Abstract Cancer stem cells (CSCs) play a pivotal role in the pathogenesis of human cancers. Previous studies have highlighted long non-coding RNA (lncRNA) modulating stemness CSCs. In our investigation, we identified an upregulation lncRNA FOXD1-AS1 The enforced expression promotes tumorigenesis and self-renewal pancreatic cancer Conversely, knockdown inhibits Furthermore, findings reveal that enhances CSCs by up-regulating osteopontin/secreted phosphoprotein 1(SPP1) acting as ceRNA to...

The objective of this study was to compare the osteogenic potential human embryonic stem cells (hESCs) within two‐ and three‐dimensional (2D 3D) culture systems. hESCs H1 line (Wicell Inc., Madison, Wisc., USA) were induced form embryoid bodies (EBs) through 5 days suspension non‐adherent dishes. Following enzymatic dissociation, EB‐derived single seeded on either novel 3D porous PLGA scaffolds or 2D dishes with same total cell number. Osteogenic differentiation media supplemented...

Human embryonic stem cells (hESCs) are considered as useful tools for pre-clinical studies in regenerative medicine. Although previous reports have shown direct chondrogenic differentiation of mouse and hESCs, low yield cellular heterogenicity the resulting cell population impairs generation sufficient numbers differentiated further testing applications. Based on our previously established high-density micromass model system to study hESC chondrogenesis, we evaluated effects transforming...

Bone repair and regeneration can be enhanced through implantation of biocompatible biodegradable scaffolds, which serve primarily as osteoconductive moieties. In this study, the mechanical properties microenviroment 3D printed poly-lactic-co-glycolic acid (PLGA) scaffolds are examined. Additionally, proliferation differentiation human fetal osteoblasts evaluated after 3 weeks in vitro culture on scaffolds. The results showed that PLGA examined had similar to trabecular bone, but was still...

Endothelial progenitors derived from human embryonic stem cells (hESCs) hold much promise in clinical therapy. Conventionally, lineage-specific differentiation of hESCs is achieved through supplementation various cytokines and chemical factors within the culture milieu. Nevertheless, this a highly inefficient approach that often limited by poor replicability. An alternative genetic modulation with recombinant DNA. Hence, study investigated whether transduction an adenoviral vector expressing...

Synthesis of a novel unnatural amino acid (2-FMPT) for the solid-phase synthesis peptide-based probes suitable target-specific activity-based profiling protein tyrosine phosphatases from crude proteomes is reported.

Mesenchymal stromal cells (MSCs) possess both immuno-privileged and immuno-inhibitory properties that contribute to their therapeutic effects. Ex vivo expansion is required obtain sufficient for therapy, but might also alter immunological properties. To date there has been no systematic study of MSC immunobiology during extended culture. Here, we demonstrate immuno-privilege immunosuppressive MSCs change with increasing passage. We although exhibit powerful effects through secretion...

Applications of human embryonic stem cells (hESCs) are limited by the use mouse fibroblasts feeder and animal-derived components during culture. In this study, we demonstrated potential extracellular matrix (ECM) derived from autologous feeders to support long-term undifferentiated growth hESCs in xeno-free, serum-free, feeder-free conditions. Autologous H9 ebF (feeder outgrowth embryoid body [EB] predifferentiated hESCs) was EBs through a direct-plating system. The ECM comprising collagen...

In this paper, the buffer-related current collapse (CC) in AlGaN/GaN HEMTs is investigated by using pulsed I-V measurements and 2-D drift-diffusion simulations. The simulation results indicate that negative potential (NP), appearing Fe-doped buffer under gate, found to account for CC. NP will induce threshold voltage shifts, which an inflection point (IP) of found. It could be revealed characteristics are significant depict trapping effect when building a compact large-signal model....

The α, β and γ isoforms of mammalian heterochromatin protein 1 (HP1) selectively bind to methylated lysine 9 histone H3 via their chromodomains. Although the phenotypes HP1-knockout mice are distinct for each isoform, molecular mechanisms underlying HP1 isoform-specific function remain elusive. In present study, we found that in contrast HP1α, HP1γ could not tri-methylated a reconstituted tetra-nucleosomes when nucleosomes were an uncompacted state. hinge region connecting HP1's chromodomain...

Abstract Zinc anode suffers from tough issues such as dendrite, corrosion, and hydrogen evolution, which lead to premature battery failure thus restrict the practical application of aqueous zinc‐metal batteries (ZMABs). Herein, a polydentate‐ligand tactic is introduced reconstruct solvation structure, improve corrosion resistance, trigger selective dendrite‐free deposition via β‐Alanyl‐L‐histidine (AH). With abundant amino, amide, carboxyl, imidazolyl groups, AH shows chelation effect,...

Cell density has profound impacts on the cell culture practices of human pluripotent stem cells.The regulation growth, death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act this stage.We use a chemically defined medium to systemically examine cellular activities impact components in high-density culture.We show that acidosis main factor alters cycle, gene expression metabolism density.The low pH leads inhibition...

A True Random Number Generator is an essential component in data encryption, hardware security, physical unclonable functions, and statistical analyses. Conventional CMOS devices usually exploit the thermal noise or jitter to generate randomness, which suffers from high energy consumption, slow bit generating rate, large area, over-complicated circuit. In this mini review, we introduce novel randomness mechanism based on stochastic switching behavior of magnetic tunnel junctions. As compared...

Abstract Background Osimertinib is the first-line therapy for patients with non-small cell lung cancer harboring epidermal growth factor receptor–activating alterations. Although osimertinib has been shown to elicit profound patient responses, cells frequently develop additional alterations that sustain their proliferation capacity. This acquired resistance represents a substantial hurdle in precision medicine cancer. Methods The biological and cellular properties of G-quadruplex ligand...

Autologous feeder cells have been developed by various methods to minimize the presence of xenogenic entities in human embryonic stem cell (hESC) cultures. However, there was no systematic comparison supportive effects on hESC growth, nor feeder-free culture systems and standard mouse cells. In this study, we aimed compare abilities autologous feeders derived either directly from H9 hESCs (H9 dF) or outgrowth embryoid body predifferentiated suspension ebF). Mouse system matrigel-mTeSR1 were...

Current bone disease therapy with marrow-derived mesenchymal stromal cells (MSC) is hampered by low efficiency. Advanced allogeneic studies on well-established mouse genetic and models are hindered difficulties in isolating murine MSC (mMSC). And mMSC prepared from different laboratories exhibit significant heterogeneity. Hence, this study aimed to identify isolate a sub-population of at an early passage number high osteogenic potential. Enrichment was achieved 1-hr silica incubation...

Collecting sweat and monitoring its rate is important for determining body condition further analyses, as this provides vital information about physiologic status fitness level could become an alternative to invasive blood tests in the future. Presented here a one-dollar, disposable, paper-based microfluidic chip real-time of rate. The chip, pasted on any part skin surface, consists adhesive layer, sweat-proof sweat-sensing scale layer with disk-shape from bottom top. has impressed wax...

Immortalized cell lines and live animal models are commonly used for cytotoxicity screening of biomedical devices materials. However, these assays poorly reflect human physiology have numerous other disadvantages. An alternative may be to utilize differentiated fibroblastic progenies embryonic stem cells (hESC) in vitro toxicology screening. These were generated through random spontaneous differentiation within standard culture media, over several passages. The cytotoxic response the hESC...