A5068753149- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Supramolecular Chemistry and Complexes
- Molecular Sensors and Ion Detection
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Signaling Pathways in Disease
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Molecular Junctions and Nanostructures
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Neuroendocrine Tumor Research Advances
- Drug Transport and Resistance Mechanisms
- Neuroendocrine regulation and behavior
- Genomics and Chromatin Dynamics
- Toxin Mechanisms and Immunotoxins
- Neuropeptides and Animal Physiology
- Advanced Chemical Physics Studies
- Retinoids in leukemia and cellular processes
University of Wrocław
2011-2024
Johns Hopkins Medicine
2003-2005
Johns Hopkins University
2002-2005
Institute of Cancer Research
2005
Sidney Kimmel Comprehensive Cancer Center
2004
Yonsei University
2004
The Wistar Institute
2002
University of Pennsylvania
2002
Trinity College Dublin
2000
Histone acetyltransferases (HATs) use acetyl CoA to acetylate target lysine residues within histones and other transcription factors, such as the p53 tumor suppressor, promote gene activation. HAT enzymes fall into subfamilies with divergence in sequence substrate preference. Several proteins have been implicated human cancer. We previously reported on preparation of peptide-CoA conjugate inhibitors distinct specificities for p300/CBP [cAMP response element binding protein (CREB)-binding...
Histone acetyltransferase (HAT) activities of proteins such as p300, CBP, and P/CAF play important roles in activation gene expression. We now show that the HAT activity p300 can also be required for down-regulation transcription by a DNA binding repressor protein. Promyelocytic leukemia zinc finger (PLZF), originally identified fusion with retinoic acid receptor alpha rare cases all-trans-retinoic acid-resistant acute promyelocytic leukemia, is transcriptional recruits histone...
This paper describes the first potent and selective p300 histone acetyltransferase (HAT) inhibitor which is effective in live cells. compound 7 a coenzyme A analogue conjugated to cell permeabilizing oligoArg peptide via disulfide linkage. was shown block cellular acetylation transcription using p300-sensitive reporter. It should thus be broadly useful for dissecting role of HAT activity physiologic disease states.
DEK is a mammalian protein that has been implicated in the pathogenesis of autoimmune diseases and cancer, including acute myeloid leukemia, melanoma, glioblastoma, hepatocellular carcinoma, bladder cancer. In addition, appears to participate multiple cellular processes, transcriptional repression, mRNA processing, chromatin remodeling. Sub-nuclear distribution this protein, with attendant functional ramifications, remained controversial topic. Here we report undergoes acetylation vivo at...
Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with cancers in immunocompromised populations. Antiviral drugs targeted against lytic viral replication have limited efficacy these disease settings. EBV infection of peripheral blood mononuclear cells induces growth proliferation the latency virus-encoded nuclear antigen (EBNA)2 transcriptional transactivator (TAT) essential for this response. EBNA2 targets cellular DNA-binding protein CBF1 to mimic activated Notch...
The immunosuppressive cyclic nonapeptide cyclolinopeptide A inhibits calcium‐dependent, but not calcium‐independent, activation of T lymphocytes comparably to the actions cyclosporin and FK506. concentration required for complete inhibition, however, is 10 times higher than that A. In addition, we demonstrate calcineurin, a phosphatase which plays an important role in lymphocyte signalling, inhibited vitro by mechanism dependent on peptidyl‐prolyl cis‐trans isomerase (PPIase) cyclophilin...
Improving the sensitivity of detection and fragmentation peptides to provide reliable sequencing is an important goal mass spectrometric analysis. Peptides derivatized by bicyclic quaternary ammonium ionization tags: 1-azabicyclo[2.2.2]octane (ABCO) or 1,4-diazabicyclo[2.2.2]octane (DABCO), are characterized increased in electrospray spectrometry (ESI-MS) longer retention times on reverse-phase (RP) chromatography columns. The improvement limit was observed even for dissolved 10 mM NaCl....
Abstract Recently, ubiquitin was suggested as a promising anti‐inflammatory protein therapeutic. We found that peptide fragment corresponding to the 50–59 sequence (LEDGRTLSDY) possessed immunosuppressive activity comparable with of ubiquitin. CD and NMR spectroscopies were used determine conformational preferences LEDGRTLSDY in solution. The mixture, obtained by pepsin digestion ubiquitin, even more potent than intact protein. Although exhibited well‐defined conformation methanol, its...
Isotope exchange at the histidine C2 atom of imidazole in D2O solution is well known to occur a significantly slower rate than amide protons. Analysis kinetics this isotope-exchange reaction proposed herein as method detecting phosphorylation. This modification His-containing peptides challenging pinpoint because its instability under acidic conditions during CID-MS analysis. In work, we investigated effect phosphorylation side chain on deuterium-hydrogen (DHX) imidazole. The results...
Abstract Argireline‐containing cosmetics attract public interest due to their confirmed reduction of facial wrinkles. Argireline is a peptide that works by inhibiting the release neurotransmitters in neuromuscular junction, producing botox‐like effect. Therefore, it used as safe needle‐free alternative botox treatment. In this work we investigated presence cosmetic creams and sera application reversed phase liquid chromatography tandem mass spectrometry (RP‐HPLC/MS MS/MS). The analysis...
A replacement of both Cys residues by His in oxytocin (OXT) sequence allows for the formation stable complex with {NH(2), N(Im), N(Im(macrochelate))} binding mode at physiological pH. The detailed potentiometric and spectroscopic studies on Cu(II) complexes [His(1,6)]OXT, together high resolution NMR investigations 3D structures [His(1,6)]OXT [His(1,6)]AVP analogues are presented discussed. Exchange Cys-S-S-Cys bridge His-Cu(II)-His motif is very promising, because resulting retain...
Proteinogenic amino acids were derivatized via coupling reactions with N-hydroxysuccinimide esters of Safirinium dyes. The obtained compounds fully characterized by 1H and 13C NMR, IR, ESI-MS spectral techniques. Elemental analysis, as well the crystal structure analysis exemplary compounds, performed. absorption fluorescent properties derivatives have been systematically investigated. optical very good water solubility allowed for development a simple method determination means micellar...