A5086761342- Metabolism and Genetic Disorders
- Monoclonal and Polyclonal Antibodies Research
- Folate and B Vitamins Research
- Lysosomal Storage Disorders Research
- Complement system in diseases
- Metabolomics and Mass Spectrometry Studies
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Amino Acid Enzymes and Metabolism
- Trypanosoma species research and implications
- Immunodeficiency and Autoimmune Disorders
- Muscle metabolism and nutrition
Codexis (United States)
2022-2023
The emergence of new therapeutic modalities requires complementary tools for their efficient syntheses. Availability methodologies site-selective modification biomolecules remains a long-standing challenge, given the inherent complexity and presence repeating residues that bear functional groups with similar reactivity profiles. We describe bioconjugation strategy native peptides relying on high site selectivity conveyed by enzymes. engineered penicillin G acylases to distinguish among free...
Fabry disease is caused by a deficiency of α-galactosidase A (GLA) leading to the lysosomal accumulation globotriaosylceramide (Gb3) and other glycosphingolipids. patients experience significant damage heart, kidney, blood vessels that can be fatal. Here we apply directed evolution generate more stable GLA variants as potential next generation treatments for disease. GLAv05 GLAv09 were identified after screening than 12,000 through 8 rounds evolution. Both exhibit increased stability at both...
Classical homocystinuria (HCU) is a rare inborn error of amino acid metabolism characterized by accumulation homocysteine, an intermediate product methionine metabolism, leading to significant systemic toxicities, particularly within the vascular, skeletal, and ocular systems. Most patients require lifelong dietary therapy with severe restriction natural protein minimize intake, many still struggle maintain healthy homocysteine levels. Since eliminating from diet reduces levels, we...
Immunoglobulin-degrading proteases are secreted by pathogenic bacteria to weaken the host immune response, contributing evasion mechanisms during an infection. Proteases specific IgG and IgA immunoglobulin classes have previously been identified characterized, only a single report exists on porcine IgM-degrading enzyme. It is unclear whether human pathogens also produce enzymes that can break down IgM. Here, we four novel from different genera of human-infecting bacterial pathogens. All...
Maple syrup urine disease (MSUD) is an inborn error of branched-chain amino acid metabolism affecting several thousand individuals worldwide. MSUD patients have elevated levels plasma leucine and its metabolic product α-ketoisocaproate (KIC), which can lead to severe neurotoxicity, coma, death. Patients must maintain a strict diet protein restriction medical formula, periods noncompliance or illness acute decompensation cumulative neurological impairment. Given the lack therapeutic options...