A5046964714- Computational Drug Discovery Methods
- Reproductive Biology and Fertility
- Cell Image Analysis Techniques
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
University of Dundee
2023-2025
Ninewells Hospital
2023-2024
In this article, we detail our latest findings towards developing diversified series of potential non-hormonal contraceptive compounds using a phenotypic screening approach against human sperm. Phenotypic nine compound libraries (88,773 in total) was conducted an in-house automated robotic platform, allowing quantification sperm motility samples pre-treated with the compounds. From these screens, 9 chemical were identified and investigated hit expansion programs, particular focus on...
Male contraceptive options and infertility treatments are limited, almost all innovation has been limited to updates medically assisted reproduction protocols methods. To accelerate the development of drugs that can either improve or inhibit fertility, we established a small molecule library as toolbox for assay screening campaigns using human spermatozoa. We have profiled compounds in Sperm Toolbox several automated high-throughput assays measure stimulation inhibition sperm motility...
Abstract Male contraceptive options and infertility treatments are limited. To help overcome these limitations we established a small molecule library as toolbox for assay development screening campaigns using human spermatozoa. We have profiled all compounds in our automated high-throughput platform provide the dataset, which allows direct side-by-side comparisons of compound effects assays live
ABSTRACT We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of functional state sperm. use this gain insight into mechanism action compounds that modify sperm function as a platform for contraceptive discovery.