A5082197627- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Advanced Fluorescence Microscopy Techniques
- Stress Responses and Cortisol
- Advanced Electron Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Genetics and Neurodevelopmental Disorders
- Tryptophan and brain disorders
- Autoimmune Neurological Disorders and Treatments
University of Maryland, Baltimore
2015-2024
Nikon (United States)
2023
MAGUK scaffold proteins play a central role in maintaining and modulating synaptic signaling, providing framework to retain position receptors, signaling molecules, other components. In particular, the MAGUKs SAP102 PSD-95 are essential for function at distinct developmental timepoints perform both overlapping unique roles. While their similar structures allow common binding partners, is expressed earlier synapse development required synaptogenesis, whereas expression peaks later associated...
Abstract NMDA receptor (NMDAR) activation is critical for maintenance and modification of synapse strength. Specifically, NMDAR by spontaneous glutamate release has been shown to mediate some forms synaptic plasticity as well development. Interestingly, there evidence that within individual synapses each mode may be segregated such postsynaptically are distinct pools responsive receptors. To examine potential regulators because in cultured hippocampal neurons, we used GCaMP6f imaging at...
The NMDA receptor (NMDAR) subunit GluN1 is critical for function and plays a pivotal role in synaptic plasticity. Mounting evidence has shown that pathogenic autoantibody targeting of the NMDARs, as anti-NMDAR encephalitis, leads to altered NMDAR trafficking localization. However, underlying signaling pathways affected by antibodies remain be fully delineated. It remains unclear whether patient influence transmission via direct effects on channel function. Here, we show using short-term...
Summary Action potentials trigger neurotransmitter release with minimal delay. Active zones mediate this temporal precision by co-organizing primed vesicles Ca V 2 2+ channels. The presumed model is that scaffolding proteins directly tether to 2s. We find clustering and vesicle priming are executed separate machineries. At hippocampal synapses, nanoclusters positioned at variable distances from those of the protein Munc13. active zone organizer RIM anchors both proteins, but distinct...
Tight coordination of the spatial relationships between protein complexes is required for cellular function. In neuronal synapses, many proteins responsible neurotransmission organize into subsynaptic nanoclusters whose trans-cellular alignment modulates synaptic signal propagation. However, these and NMDA receptors (NMDARs), which are learning memory, remain undefined. Here, we mapped relationship key NMDAR subunits to reference in active zone postsynaptic density using multiplexed...
The MAGUK family of scaffold proteins plays a central role in maintaining and modulating synaptic signaling, providing framework to retain position receptors, signaling molecules, other components. Of these proteins, SAP102 PSD-95 are essential for function at distinct developmental timepoints perform overlapping as well unique roles. While their similar structures allow common binding partners, is expressed earlier synapse development required synaptogenesis, whereas expression peaks later...
Abstract NMDA receptor (NMDAR) activation is critical for maintenance and modification of synapse strength. Specifically, NMDAR by spontaneous glutamate release has been shown to mediate forms synaptic plasticity as well development. Interestingly, there evidence that within individual synapses each mode may be segregated such postsynaptically are distinct pools responsive receptors. In order examine potential regulators due in cultured rat hippocampal neurons, we utilized GCaMP6f imaging at...