A5100429834- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Gene Regulatory Network Analysis
- DNA and Nucleic Acid Chemistry
- Forensic and Genetic Research
- Genetic diversity and population structure
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Environmental Sustainability in Business
- Gene expression and cancer classification
- Sustainable Supply Chain Management
- Receptor Mechanisms and Signaling
- Advanced Proteomics Techniques and Applications
- Animal Genetics and Reproduction
- Computational Drug Discovery Methods
- Plant Taxonomy and Phylogenetics
- Yersinia bacterium, plague, ectoparasites research
- Genetic and phenotypic traits in livestock
- Plant and animal studies
- Plant Disease Resistance and Genetics
Shenzhen University
2025
China Jiliang University
2024
Xi'an Jiaotong University
2020-2021
Lanzhou University
2020
National Center for Biotechnology Information
2016-2019
National Institutes of Health
2018
Shanxi Agricultural University
2017
Texas A&M University
2015
Aptamer-drug conjugates (ApDCs) have the potential to improve therapeutic index of traditional chemotherapeutic agents due their ability deliver cytotoxic drugs specifically cancer cells while sparing normal cells. This study reports on conjugation an aptamer previously described by our group, pancreatic RNA P19. To this end, P19 was incorporated with gemcitabine and 5-fluorouracil (5-FU), or conjugated monomethyl auristatin E (MMAE) derivative maytansine 1 (DM1). The ApDCs P19-dFdCMP...
Aptamers, short RNA or DNA molecules that bind distinct targets with high affinity and specificity, can be identified using high-throughput systematic evolution of ligands by exponential enrichment (HT-SELEX), but scalable analytic tools for understanding sequence-function relationships from diverse HT-SELEX data are not available. Here we present AptaTRACE, a computational approach leverages the experimental design protocol, secondary structure, potential presence many motifs to identify...
ABSTRACT As sustainability challenges, such as environmental pollution, become increasingly severe, businesses must balance stakeholder interests while achieving economic objectives. Applying theory, this study finds that active participation in corporate social responsibility (CSR) significantly improves supply chain efficiency (SCE), with an indirect effect through mechanisms green technology innovation and information sharing. Heterogeneity experiments reveal CSR has a greater on SCE...
Gene regulatory networks (GRNs) describe relationships between transcription factors (TFs) and their target genes. Computational methods to infer GRNs typically combine evidence across different conditions context-agnostic networks. We develop a method, Network Reprogramming using EXpression (NetREX), that constructs context-specific GRN given expression data prior network. NetREX remodels the network obtain topology provides best explanation for data. Because utilizes topology, we also...
Premise of the study: Proso millet ( Panicum miliaceum ; Poaceae) is a minor crop with good nutritional qualities and strong tolerance to drought stress soil infertility. However, studies on genetic diversity have been limited due lack efficient markers. Methods: Illumina sequencing technology was used generate short read sequences proso millet, de novo transcriptome assemblies were develop assembly millet. Genic simple sequence repeat (SSR) markers identified detect polymorphism among 56...
Abstract Background Flower longevity plays an important role in pollen dispersal and reproductive success plants. In dichogamous plants, the duration of anthesis as well time allocated to male female functions can vary response intrinsic factors (e.g., flowering resource allocation) pollination context along a growth season. However, fitness consequences phenological dynamics have rarely been examined. This study aims unravel potential causes driving variation flower longevity, sex phases,...
RNA-based therapeutics, i.e. the utilization of synthetic RNA molecules to alter cellular functions, have potential address targets which are currently out scope for traditional drug design pipelines. This however hinges on ability selectively deliver and internalize therapeutic RNAs into cells interest. Cell internalizing aptamers selected against surface receptors discriminatively expressed target hold particular promise as suitable candidates such delivery agents. Specifically, these can...
Ancestry informative markers have extensive uses and advantages in inferring ancestral origins estimating genetic information components of admixed populations. With the characteristics highly cultural exchange structure Kyrgyz group, it is essential to enrich data group. In this study, we used a self-developed ancestry marker-deletion/insertion polymorphic (AIM-DIP) panel explore Chinese group population relationships between reference Results showed that all AIM-DIP loci were conformed...
Neuron-specific enolase (NSE) is currently the most reliable biomarker for small cell lung cancer (SCLC), which important disease monitoring, clinical evaluation and diagnosis. However, traditional methods suffer from various disadvantages, including instability, complexity, time-consuming operations, necessity standards. In this study, we developed a calibration-free concentration analysis (CFCA) method based on surface plasmon resonance (SPR) technology, to accurately quantify active of...
Abstract Aptamers, short synthetic RNA/DNA molecules binding specific targets with high affinity and specificity, are utilized in an increasing spectrum of bio-medical applications. Aptamers identified vitro via the Systematic Evolution Ligands by Exponential Enrichment (SELEX) protocol. SELEX selects binders through iterative process that, starting from a pool random ssDNA/RNA sequences, amplifies target-affine species series selection cycles. HT-SELEX, which combines throughput sequencing,...
Short tandem repeats (STRs) play an essential role in forensic genetics due to their high degree of polymorphisms, wide distributions and easy detection method. In this study, allelic frequencies statistical parameters the 19 autosomal STR loci a Kazak ethnic group were calculated, its genetic relationships with reference populations assessed order understand population structure better enrich data for practice Chinese group. There 226 identified alleles corresponding ranging from 0.0008...
Abstract Understanding gene regulation is a fundamental step towards understanding of how cells function and respond to environmental cues perturbations. An important in this direction the ability infer transcription factor (TF)-gene regulatory network (GRN). However networks are typically constructed disregarding fact that programs conditioned on tissue type, developmental stage, sex, other factors. Due lack biological context specificity, these context-agnostic may not provide insight for...
We propose a computational framework to identify functional modules in multiple networks that can be constructed from different types of "omics" data through incorporating network topology with constitutional similarity between nodes representing biomolecules across networks. With the help mixed integer programming, outputs locally optimized modules, which either conserved or network-specific. apply four yeast protein-protein interaction and compare several state-of-the-art algorithms. The...
Aptamers, short synthetic RNA/DNA molecules binding specific targets with high affinity and specificity, are utilized in an increasing spectrum of bio-medical applications. Aptamers identified vitro via the Systematic Evolution Ligands by Exponential Enrichment (SELEX) protocol. SELEX selects binders through iterative process that, starting from a pool random ssDNA/RNA sequences, amplifies target-affine species series selection cycles. HT-SELEX, which combines throughput sequencing, has...
Abstract Synthetic RNA molecules are increasingly used to alter cellular functions. These successful applications indicate that RNA-based therapeutics might be able target currently undruggable genes. However, achieve this promise, an effective method for delivering therapeutic RNAs into specific cells is required. Recently, aptamers emerged as promising delivery agents due their ability of binding cell receptors. Crucially, these can frequently internalized the expressing receptors on...
The identification of sub-populations cells present in a sample and the comparison such across samples are among most frequently performed analyzes single-cell data. Current tools for these kinds data, however, fall short their ability to adequately perform tasks. We introduce novel method, PopCorn (single cell sub-Populations Comparison), allowing within individual experiments while simultaneously performing mapping experiments. utilizes several algorithmic solutions enabling execution...
Summary: The AptaBlocks Web Interface is focused on providing graphical, intuitive, and platform independent access to AptaBlocks, an experimentally validated algorithmic approach for the in-silico design of oligonucleotide sticky bridges. availability online nucleic acid research community at large makes this software a highly effective tool accelerating development novel based drugs other biotechnologies. Availability: freely available...