A5016482982- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Advanced Electron Microscopy Techniques and Applications
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- Phagocytosis and Immune Regulation
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- Advanced Fluorescence Microscopy Techniques
- CAR-T cell therapy research
- HER2/EGFR in Cancer Research
- Cell Image Analysis Techniques
- Prosthetics and Rehabilitation Robotics
- Innovations in Concrete and Construction Materials
- Receptor Mechanisms and Signaling
- Mechanical Circulatory Support Devices
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Biomedical and Engineering Education
- Complement system in diseases
University of New Mexico
2017-2024
Novartis (Switzerland)
2008
SARS-CoV-2 infection depends on binding its spike (S) protein to angiotensin-converting enzyme 2 (ACE2). The S expresses an RGD motif, suggesting that integrins may be co-receptors. Here, we UV-inactivated and fluorescently labeled the envelope membrane with octadecyl rhodamine B (R18) explore role of integrin activation in mediating cell entry productive infection. We used flow cytometry confocal microscopy show
Abstract The use of therapeutic monoclonal antibodies is constrained because single antigen targets often do not provide sufficient selectivity to distinguish diseased from healthy tissues. We present HexElect ® , an approach enhance the functional by making their activity dependent on clustering after binding two different antigens expressed same target cell. lmmunoglobulin G (lgG)-mediated membrane receptors naturally occurs cell surfaces trigger complement- or cell-mediated effector...
Filoviruses are highly virulent pathogens capable of causing severe disease. The glycoproteins filoviruses the only virally expressed proteins on virion surface and required for receptor binding. As such, they main candidate vaccine antigen. Despite their virulence, most not comprehensively characterized, relatively few commercially produced reagents available study. Here, we describe two methods production purification filovirus in insect mammalian cell lines. Considerations expression...
Fluorescence single molecule imaging comprises a variety of techniques that involve detecting individual fluorescent molecules. Many these localizing molecules with precisions below the diffraction limit, which limits spatial resolution (visible) light-based microscopes. These methodologies are widely used to image biological structures at nanometer scale by fluorescently tagging interest, elucidating details behavior observed. Two common single-molecule localization microscopy (SMLM),...
Abstract Cellular entry of coronaviruses depends on binding the viral spike (S) protein to a specific cellular receptor, angiotensin-converting enzyme 2 (ACE2). Furthermore, expresses an RGD motif, suggesting that cell surface integrins may be attachment co-receptors. However, using infectious SARS-CoV-2 requires biosafety level 3 laboratory (BSL-3), which limits techniques can used study mechanism entry. Here, we UV-inactivated and fluorescently labeled envelope membrane with octadecyl...
Immunoreceptor tyrosine-based activation motif (ITAM)-containing Fc receptors are critical components of the innate and adaptive immune systems. FcεRI mediates allergic response via crosslinking IgE-bound by multivalent antigens. Yet, underlying molecular mechanisms that govern to specific antigens remain poorly understood. We compared responses induced two with distinct geometries, high valency DNP-BSA trivalent DF3, found unique secretion receptor phosphorylation profiles due differential...