A5012907726- Fungal and yeast genetics research
- Cellular transport and secretion
- Silk-based biomaterials and applications
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Enzyme Production and Characterization
- Diabetes Treatment and Management
- Enzyme Catalysis and Immobilization
- Pancreatic function and diabetes
- Fermentation and Sensory Analysis
- Corneal Surgery and Treatments
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Cancer Research and Treatments
- Neuropeptides and Animal Physiology
- Protein Interaction Studies and Fluorescence Analysis
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- Studies on Chitinases and Chitosanases
- Transgenic Plants and Applications
Kurchatov Institute
2019-2024
Genetika
2010-2020
University of Pennsylvania
2002-2005
University of Alberta
1999
Institute of Molecular Genetics
1994
Polarized growth in Saccharomyces cerevisiae is thought to occur by the transport of post-Golgi vesicles along actin cables daughter cell, and subsequent fusion with plasma membrane. Previously, we have shown that Msb3p Msb4p genetically interact Cdc42p display a GTPase-activating protein (GAP) activity toward number Rab GTPases vitro. We show here regulate exocytosis functioning as GAPs for Sec4p vivo. Cells lacking GAP displayed secretory defects, including accumulation 80–100 nm diameter....
The budding yeast Saccharomyces cerevisiae initiates polarized growth or once per cell cycle at a specific time of the and location on surface. Little is known about molecular nature temporal spatial regulatory mechanisms. It also unclear what factors, if any, among numerous proteins required to make bud are involved in determination frequency. Here we describe class cdc42 mutants that produce multiple buds random locations surface within one nuclear cycle. critical mutation responsible for...
Cytokinesis in Saccharomyces cerevisiae involves coordination between actomyosin ring contraction and septum formation and/or targeted membrane deposition. We show that Mlc1p, a light chain for Myo2p (type V myosin) Iqg1p (IQGAP), is the essential Myo1p, only type II myosin S. cerevisiae. However, disruption or reduction of Mlc1p–Myo1p interaction by deleting Mlc1p binding site on Myo1p point mutation MLC1, mlc1-93, did not cause any obvious defect cytokinesis. In contrast, different...
In Saccharomyces cerevisiae, polarized growth depends on interactions between the actin cytoskeleton and secretory machinery. Here we show that Rab GTPase-activating proteins (GAPs) Msb3 Msb4 interact directly with Spa2, a scaffold protein of "polarisome" also interacts formin Bni1. Spa2 is required for localization at bud tip. We bind specifically to Cdc42-GDP Rho1-GDP in vitro Rho GDP dissociation inhibitor act independently but oppositely Cdc42. Finally, are involved Bni1-nucleated...
Integral membrane proteins associated with the nuclear pore complex (NPC) are likely to play an important role in biogenesis of this structure. Here we have examined functional roles domains yeast protein Pom152p establishing its topology and interactions other NPC proteins. The was evaluated by alkaline extraction, protease protection, endoglycosidase H sensitivity assays. results these experiments suggest that contains a single transmembrane segment N terminus (amino acid residues 1–175)...
The essential GAB1 gene, which encodes an endoplasmic reticulum (ER)-membrane protein, was identified in a screen for mutants defective cellular morphogenesis. A temperature-sensitive gab1 mutant accumulates complete glycosylphosphatidylinositol (GPI) precursors, and its temperature sensitivity is suppressed differentially by overexpression of different subunits the GPI transamidase, from strong suppression Gpi8p Gpi17p, to weak Gaa1p, no Gpi16p. In addition, both Gab1p Gpi17p localize ER...
Rho-family GTPases Cdc42p and Rho1p play critical roles in the budding process of yeast Saccharomyces cerevisiae. However, it is not clear how functions these are coordinated temporally spatially during this process. Based on its ability to suppress cdc42-Ts mutants when overexpressed, a novel gene PXL1 was identified. Pxl1p resembles mammalian paxillin, which involved integrating various signaling events at focal adhesion. Both proteins share amino acid sequence homology structural...
This article presents the results of studying impact recombinant spidroin hydrogel on posterior epithelial cells and human corneal keratocytes in an vitro experiment. The World Health Organization its studies has established a high prevalence injuries among population developing countries. In recent years, various technologies have been proposed to restore damaged surface cornea. use biodegradable silk-based materials, such as spidroins is one main parts scientific research regeneration....
It was shown that unlike eukaryotic ubiquitin-specific proteinases (DUBs deubiquitinating enzymes) deubiquitinase ElaD E.coli does not need obligatory presence of GlyGly dipeptide at the processing site and is capable to process sites GlyAla with equal efficiency. At same time similar DUB bacterial enzyme dipeptides AlaGly, AlaAla or GlyPro. Considering functional mission agent E. coli, decrease in its specificity can be considered as valuable evolutional acquisition allowing expand a list...
Mutant variants of mini-intein PRP8 from Penicillium chrysogenum (Int4b) with improved control C-terminal processing were characterized. The presented can serve as a basis for self-removed polypeptide tags capable carrying an affine label and allowing to optimize the process obtaining target proteins peptides in E. coli cells. They allow synthesize molecules composition soluble insoluble hybrid (fusions), provide their afnne purification, autocatalytic mature products. have number features...