A5088304365- Caveolin-1 and cellular processes
- RNA Research and Splicing
- Ion Transport and Channel Regulation
University of Virginia
2023
PI3Kα is a potent oncogene that converts PIP2 to PIP3 at the plasma membrane upon activation by receptor tyrosine kinases and Ras GTPases. In absence of any structures activated PI3Kα, molecular details its remain unknown. Here, we present cryo-EM PI3Kα/KRas complex embedded in lipid nanodiscs, revealing rich ensemble states adopted surface. The sequential addition bilayer, an activating phosphopeptide leads progressive release key inhibitory domains from catalytic core, which directly...
Caveolin-1 (CAV1) is a membrane-sculpting protein that oligomerizes to generate flask-shaped invaginations of the plasma membrane known as caveolae. Mutations in CAV1 have been linked multiple diseases humans. Such mutations often interfere with oligomerization and intracellular trafficking processes required for successful caveolae assembly, but molecular mechanisms underlying these defects not structurally explained. Here, we investigate how disease-associated mutation one most highly...