A5065937777- Spaceflight effects on biology
- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
ETH Zurich
2022-2024
Abstract Dysfunctional extracellular matrices (ECM) contribute to aging and disease. Repairing dysfunctional ECM could potentially prevent age-related pathologies. Interventions promoting longevity also impact gene expression. However, the role of composition changes in healthy remains unclear. Here we perform proteomics in-vivo monitoring systematically investigate (matreotype) during C. elegans revealing three distinct collagen dynamics. Longevity interventions slow stiffening prolong...
Abstract Although it is postulated that dysfunctional extracellular matrices (ECM) drive aging and disease, how ECM integrity assures longevity unknown. Here, using proteomics in-vivo monitoring of fluorescently tagged proteins, we systematically examined the composition during Caenorhabditis elegans revealing three distinct collagen dynamics. We show age-dependent stiffening inert was slowed by interventions through prolonged replenishing collagens. In genetic automated lifespan screens for...
Motivation The difficulties in producing cell-free translation systems from different cell types limit the ability to study regulatory mechanisms that depend on biological contexts. Developing tailored diverse would be instrumental investigating cell-type-specific translational control, co- and post-translational modifications, viral manipulation strategies. Our method addresses this gap by providing a scalable adaptable solution for high-quality lysates reflect specific needs of types....