A5028759860- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Renal and related cancers
- HVDC Systems and Fault Protection
- Superconducting Materials and Applications
- Hormonal and reproductive studies
- Physics of Superconductivity and Magnetism
- Food Safety and Hygiene
- Wildlife Ecology and Conservation
- Coconut Research and Applications
Instituto Federal Goiano
2023
Universidade de São Paulo
2003-2013
The University of Queensland
2004
Universidade Federal de Minas Gerais
2004
Tohoku University
2004
Dankook University
2004
Colorado State University
2004
Universidade Brasil
2003
Cryopreservation of mammalian embryos is an important tool for the application reproductive biotechnologies. Subjective evaluation to determine embryo viability often used. The determination best cryopreservation protocol depends on morphological and molecular analysis cellular injuries. main objective this study was compare two methods by assessing alterations frozen using light, fluorescence, transmission electron microscope. Fresh (control), slow frozen, vitrified mouse were composed. To...
The present study examined the in vitro and vivo development of bovine nuclear-transferred embryos. A fetal fibroblast culture was established used as nucleus donor. Slaughterhouse oocytes were matured for 18 h before enucleation. Enucleated fused with fibroblasts an electric stimulus treated cytochalasin D cycloheximide 1 followed by alone 4 h. Reconstructed embryos cultured 7-9 days those which developed to blastocysts transferred recipient cows. Of 191 enucleated oocytes, 83 (43.5%)...
For food to be considered healthy, among other aspects, it must free of contamination that could lead the development foodborne diseases (FBDs). The hygienic and sanitary aspects consumed by population as well its incorrect handling procedures can cause FBDs. Many contaminated foods seem have normal sensory characteristics, without changes in texture, flavor, odor, so often those who consume do not perception understanding appears perfect visibly brings risk internally when consumed, which...
Recently, progress has been achieved in reproduction biotechnology with coconut water as semen extender for domestic animals and bovine oocyte vitro maturation embryo culture. The aim of this study was to evaluate the dehydrated (ACP250) its effect on sperm capacitation swine. Eighteen ejaculates were collected from three crossbred boars Landrace Duroc. Semen samples extended ACP250 solution or Beltsville Thawing Solution (BTS) submitted after extension (EXT) cooling (COO) at 16°C 24 h....
The aim of this study was to compare two ethylene glycol (EG) equilibration procedures for the quick freezing in vitro-produced bovine embryos. Cumulus-oocyte complexes (COCs) were collected from slaughterhouse ovaries. COCs matured TCM199 containing 10% fetal serum, LH, FSH and E2, fertilized. Presumptive zygotes co-cultured with a granulosa cell monolayer, at 39°C humidified atmosphere 5% CO2 air. Grade 1, expanded blastocysts (n=761) selected 7 9 days after insemination randomly...
Adult skin and fetal fibroblasts are the most frequently used donor cell types for bovine cloning by nuclear transfer (NT) but there few reports concerning Nelore cattle. The aim of this study was to evaluate in vitro vivo viability embryos reconstructed with Metaphase II oocytes differentiated somatic cells (adult ear fibroblasts). Oocytes from ovaries collected at slaughterhouse were matured 17h. Enucleation conducted aspiration first polar body (PB) small volume cytoplasm containing...
Butyrolactone I and cycloheximide specifically inhibit MPF activation prevent the resumption of meiosis. The aim this study was to investigate kinetics in vitro maturation butyrolactone I- cycloheximide-treated swine oocytes an attempt produce cytoplasts for nuclear transfer. Oocytes from slaughterhouse ovaries were randomly allotted one 3 treatments; group 1 (n = 102 – control 22 hours TCM199 supplemented with 3.05 mM glucose, 0.91 sodium pyruvate, 10% follicular fluid, 0.57 cysteine, 10...
Sloths are animals that suffer with the destruction and fragmentation of forests. They experience a low population growth rate need to be studied further for preservation species. The objective this study was contribute data relevant reproductive physiology species, selecting semen collection method evaluating seminal characteristics have never before been described in literature. Fifteen Bradypus tridactylus males were captured Manaus, Brazil. Nine them during first half 2004 (Group 1)...