A5086185481- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
BioNTech (Germany)
2024
Philipps University of Marburg
2019
The expanding field of extracellular vesicle (EV) research needs reproducible and accurate methods to characterize single EVs. Nanoparticle Tracking Analysis (NTA) is commonly used determine EV concentration diameter. As the lacking easily confirm validate NTA data, questioning reliability measurements remains highly important. In this regard, a comparison addressing measurement quality between different devices such as Malvern's NanoSight NS300 or Particle Metrix' ZetaView has not yet been...
We present the preclinical pharmacology of BNT142, a lipid nanoparticle (LNP)-formulated RNA (RNA-LNP) encoding T cell-engaging bispecific antibody that monovalently binds cell marker CD3 and bivalently claudin 6 (CLDN6), an oncofetal antigen is absent from normal adult tissue but expressed on various solid tumors. Upon BNT142 RNA-LNP delivery in culture, mice, cynomolgus monkeys, translated, followed by self-assembly into secretion functional RiboMab02.1. In vitro, RiboMab02.1 mediated...
The peritoneal fluid (ascites), replete with abundant tumor-promoting factors and extracellular vesicles (EVs) reflecting the tumor secretome, plays an essential role in ovarian high-grade serous carcinoma (HGSC) metastasis immune suppression.A comprehensive picture of mediators impacting HGSC progression is, however, not available.Methods: Proteins ascites from patients were quantified by aptamer-based SOMAscan affinity proteomic platform.SOMAscan data analyzed bioinformatic methods to...