A5100847208- Animal Genetics and Reproduction
- Axon Guidance and Neuronal Signaling
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Shoulder Injury and Treatment
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Sports injuries and prevention
- DNA and Nucleic Acid Chemistry
- Angiogenesis and VEGF in Cancer
- Tendon Structure and Treatment
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Developmental Biology and Gene Regulation
- Mesenchymal stem cell research
- Cancer-related Molecular Pathways
- TGF-β signaling in diseases
- Genomic variations and chromosomal abnormalities
- RNA Research and Splicing
- NF-κB Signaling Pathways
- 14-3-3 protein interactions
- Liver physiology and pathology
Spaulding Rehabilitation Hospital
2024-2025
Harvard University
1994-2025
Transposagen Biopharmaceuticals (United States)
2010
Vesta Sciences (United States)
2010
Indiana University – Purdue University Indianapolis
2004
Indiana University School of Medicine
2004
The Wistar Institute
1996-1999
Columbia University
1991
Salk Institute for Biological Studies
1986-1990
University of California, San Diego
1989
Angiogenesis is critical for vascular remodeling during development and contributes to the pathogenesis of diseases such as cancer. Targeted disruption several EphB class receptor tyrosine kinases results in defects embryogenesis. The role EphA receptors remodeling, however, not well-characterized. We recently demonstrated that global inhibition disrupts endothelial migration induced by ephrin, VEGF or tumor-derived signals, though specific target remained undefined. Here, we report EphA2...
Acentric extrachromosomal elements, such as submicroscopic autonomously replicating circular molecules (episomes) and double minute chromosomes, are common early, in some cases initial, intermediates of gene amplification many drug-resistant tumor cell lines. In order to gain a more complete understanding the process, we investigated molecular mechanisms by which elements generated traced fate these over time. The model system consists Chinese hamster line (L46) created transfer initial...
While extracorporeal shockwave therapy (ESWT) may be an efficacious adjunctive treatment option for musculoskeletal injuries, current research is limited by significant heterogeneity within protocols. This study aims to establish international expert consensus recommendations on ESWT terminology, parameters, procedural considerations, contraindications and side effects in the application of sports injuries. A systematic literature search was performed use medicine injuries identify potential...
Ubiquitin-mediated degradation targets cell cycle regulators for proteolysis.Much of the ubiquitin pathway's substrate specificity is conferred by E3 ligases, and cullins are core components some E3s.CUL-4A encodes one six mammalian amplified and/or overexpressed in breast cancer, which suggests a role regulating progression.To examine CUL-4A's physiologic function, we generated CUL-4A deletion mutation mice.No viable ؊/؊ pups no homozygous mutant embryos as early 7.5 days postcoitum (dpc)...
The availability of suitable human livers for transplantation falls short the number potential patients. In addition, primary hepatocytes cell-therapy and drug development applications is significantly limited; less than 700 per year are available such studies. However, majority these organs cannot be utilized due to pathological infections (e.g., HepB, HepC, or HIV) excessive levels steatosis. Thus, cells needed cell therapy far exceeds from donated livers. ability implant progenitor...
Acentric extrachromosomal elements, such as submicroscopic autonomously replicating circular molecules (episomes) and double minute chromosomes, are common early, in some cases initial, intermediates of gene amplification many drug-resistant tumor cell lines. In order to gain a more complete understanding the process, we investigated molecular mechanisms by which elements generated traced fate these over time. The model system consists Chinese hamster line (L46) created transfer initial...
Gene amplification in human tumor cells is frequently mediated by extrachromosomal elements (e.g., double minute chromosomes [DMs]). Recent experiments have shown that DMs can be formed from smaller, submicroscopic circular precursors referred to as episomes (S. M. Carroll, L. DeRose, P. Gaudray, C. Moore, D. R. Needham-Vandevanter, Von Hoff and G. Wahl, Mol. Biol. 8:1525-1533, 1988). To investigate whether are generally involved intermediates gene amplification, we determined they mediate...
We have developed a gene transfer approach to facilitate the identification and isolation of chromosomal regions which are prone high-frequency amplification. Such identified by assaying for transformants show resistance PALA and/or methotrexate amplification vector containing genes encode enzyme targets these antiproliferative agents. 2 47 displayed transfected genes, in this report we describe analysis one them (L46). Molecular integration site transformant L46 revealed that donated were...
A variety of experimental approaches have been devised recently to mutate mammalian genes by homologous recombination. In this report, we describe the disruption Hox1.3 locus using two these approaches, namely, positive-negative selection and activation a promoterless gene. Interestingly, observe similarly high frequencies targeted with both procedures. The frequency vector was unexpected given extremely low level expression in embryonic stem cell line used for studies. These data indicate...
Eucaryotic expression vectors containing the Escherichia coli pyrB gene (pyrB encodes catalytic subunit of aspartate transcarbamylase [ATCase]) and Tn5 phosphotransferase (G418 resistance module) were transfected into a mutant Chinese hamster ovary cell line possessing CAD multifunctional protein lacking ATCase activity. G418-resistant transformants isolated analyzed for activity, ability to complement defect, resist high concentrations inhibitor N-(phosphonacetyl)-L-aspartate (PALA) by...
Reports of injury characteristics high school track and field athletes participating in jumping events the United States are limited. In this descriptive epidemiological study, we report rates patterns these athletes.