A5034088672- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Muscle metabolism and nutrition
- Pancreatic function and diabetes
- Biochemical Acid Research Studies
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Fatty Acid Research and Health
- Thyroid Disorders and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Growth Hormone and Insulin-like Growth Factors
- Amino Acid Enzymes and Metabolism
- Cardiovascular and exercise physiology
- Sirtuins and Resveratrol in Medicine
- Exercise and Physiological Responses
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- Gestational Diabetes Research and Management
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
Technical University of Munich
2023-2024
Children's Medical Research Institute
2023
The University of Sydney
2023
Sydney Children’s Hospitals Network
2023
Klinikum rechts der Isar
2023
Queen Mary University of London
2000-2014
Universidad de Londres
2005
St Bartholomew's Hospital
1997-1998
University College London
1995
University of London
1995
Abstract Although long noncoding RNAs (lncRNAs) dominate the transcriptome, their functions are largely unexplored. The extensive overlap of lncRNAs with coding and regulatory sequences restricts systematic interrogation by DNA-directed perturbation. Here we developed genome-scale lncRNA transcriptome screening using Cas13d/CasRx. We show that RNA targeting overcomes limitations inherent to other methods, thereby considerably expanding explorable space lncRNAome. By evolving system toward...
In using Western blot analysis with antibodies raised against recombinant pyruvate dehydrogenase kinase (PDK) isoforms PDK2 and PDK4, this study demonstrates selective PDK isoform switching in specific skeletal muscle types response to high-fat feeding that is associated altered regulation of activity by pyruvate. The administration a diet high saturated fats led stable (approximately 2-fold) increases activities both typical slow-twitch (soleus [SOL]) fast-twitch (anterior tibialis [AT])...
AMPK activates SIRT1 in liver and skeletal muscle. Impaired circadian function is associated with development of obesity. regulates suppressed white adipose tissue (WAT) obese patients. We examined the potential role regulation components WAT db/db mice fed a high-fat diet (HFD), investigated whether metformin-mediated activation opposed any deleterious changes clock mechanism.db/+ were administered metformin (250 mg/kg/day; 7 days). Separately, HFD for 16-weeks. 3T3-L1 adipocytes incubated...
Rapid inhibition of acetyl-CoA carboxylase (ACC) activity in rat liver response to 6 h starvation and rapid re-activation 2-6 re-feeding chow were shown be due changes the expressed existing enzyme. Decreases increases ACC concentration occurred at later stages transitions, i.e. 6-48 8-24 respectively. The decrease was primarily its phosphorylation state, demonstrated by a significantly decreased Vmax. increased Ka for citrate enzyme purified avidin-Sepharose chromatography from h- or 48...
Solid cancers like pancreatic cancer (PDAC) frequently exploit nerves for rapid dissemination. This neural invasion (NI) is an independent prognostic factor in PDAC, but insufficiently modelled genetically-engineered mouse models (GEMM) of PDAC. Here, we systematically screened human-like NI Europe's largest repository GEMM PDAC comprising 295 different genotypes. phenotype screen uncovered two with NI, which are both characterized by pancreas-specific overexpression...
A comparison was made between the time courses of restoration pyruvate dehydrogenase activities, fructose 2,6-bisphosphate concentrations and lipogenic rates, together with net hepatic glucose flux glycogen synthesis/deposition in livers 48 h-starved rats provided laboratory chow ad libitum for up to 24 h. Increased glycogenesis, lipogenesis uptake were observed after 1 h re-feeding, preceding re-activation dehydrogenase, which occurred 3-4 only 5-6 The implication temporal relationship...
We investigated the temporal relationship between hepatic glycogen depletion and cardiac PDH (pyruvate dehydrogenase complex) activities during acute phase of starvation. There was a striking correlation decline in inactivation first 10 h Re-feeding after 6 starvation associated with complete re-activation liver to approx. 75% fed value heart, whereas rats previously starved for 24-48 delayed diminished heart. The results are discussed reference fate dietary carbohydrate re-feeding.
The progressive effects of starvation on muscle glucose utilization were studied in the conscious resting rat. High rates uptake and phosphorylation constantly working cardiothoracic (heart, diaphragm) postural skeletal muscles (soleus, adductor longus) maintained for at least 9 h starvation. A rapid decline cardiac was observed during period 9-24 starvation, but other more gradual. Consequently, even after 24 h, these remained quantitatively significant. In both (postural) muscle, activity...
The mitochondrial pyruvate dehydrogenase complex (PDC) catalyses the oxidative decarboxylation of pyruvate, and links glycolysis to tricarboxylic acid cycle ATP production. Adequate flux through PDC is important in tissues with a high requirement, lipogenic (since it provides cytosolic acetyl-CoA for fatty (FA) synthesis), generating malonyl-CoA, potent inhibitor carnitine palmitoyltransferase (CPT I). Conversely, suppression activity crucial glucose conservation when scarce. This review...
In the fed state, percentages of pyruvate dehydrogenase complex (PDH) in active form (PDHa) diaphragm and a selection skeletal muscles (adductor longus, soleus, extensor digitorum tibialis anterior, gastrocnemius) ranged from 8% (soleus) to 38% (gastrocnemius). Major decreases PDHa activities all these were observed after 15 h starvation, by which time less than 40% values. general, response starvation was more rapidly high oxidative capacity. The patterns changes skeletal-muscle PDH during...
1. Protein kinase activities in homogenates of rat islets Langerhans were studied. 2. On incubation with [gamma-32P]ATP, incorporation 32P into protein occurred: this phosphorylation was neither increased by cyclic AMP nor decreased the AMP-dependent inhibitor described Ashby & Walsh [(1972) J. Biol. Chem. 247, 6637--6642]. 3. [gamma-32P]ATP and histone as exogenous substrate for phosphorylation, stimulated (approx. 2.5-fold) inhibited inhibitor. In contrast, when casein used substrate, not...
In small rats deprived of food for 19h (or 43h), 36% 39%) the glycogen that disappeared was lost from carcass and 64% 61%) liver. Carcass is potentially a substantial source glucose during short-term starvation via Cori cycle.
The concentrations of malonyl-CoA, citrate, ketone bodies and long-chain acylcarnitine were measured in freeze-clamped liver samples from fed or starved normal, partially hepatectomized sham-operated rats. These parameters used conjunction with measurements the concentration plasma non-esterified fatty acids rates hepatic lipogenesis to obtain correlations between acid delivery liver, oxidation CO2. indicated that development after partial hepatectomy is due an increased partitioning...
Glucose administration to 48 h-starved rats increased hepatic glucose, lactate, pyruvate and glycogen concentrations re-activated PDH (pyruvate dehydrogenase complex) in kidney, but not heart or liver. Dichloroacetate together with glucose all three tissues, decreased lactate impaired resynthesis. Thus on re-feeding, delayed re-activation is important for provision of precursors glyconeogenesis.
Lipoprotein lipase (LPL) activities in parametrial and interscapular adipose tissue, soleus adductor longus muscles hearts of female rats were measured during progressive starvation, chow re-feeding after 24 h starvation throughout dark light phases permitted unrestricted access to chow. Adipose-tissue LPL declined by 50% 6 continued fall as the period was extended h. Skeletal-muscle dramatically increased between 9 12 starvation. Cardiac 2.5-fold within reaching a maximum rapidly 2 ad...
The mitochondrial pyruvate dehydrogenase complex (PDC) catalyses the oxidative decarboxylation of pyruvate, and links glycolysis to tricarboxylic acid cycle ATP production. Adequate flux through PDC is important in tissues with a high requirement, lipogenic (since it provides cytosolic acetyl-CoA for fatty (FA) synthesis), generating malonyl-CoA, potent inhibitor carnitine palmitoyltransferase (CPT I). Conversely, suppression activity crucial glucose conservation when scarce. This review...