A5016581702- Plant Gene Expression Analysis
- Ion Channels and Receptors
- Genetic and Environmental Crop Studies
- Antioxidant Activity and Oxidative Stress
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant Molecular Biology Research
- Light effects on plants
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Agricultural pest management studies
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
Seoul National University
2020-2024
ABSTRACT Genome editing (GE) using CRISPR/Cas systems has revolutionized plant mutagenesis. However, conventional transgene-mediated GE methods have limitations due to the time-consuming generation of stable transgenic lines expressing Cas9/single guide RNA (sgRNA) module through tissue cultures. Virus-induced genome (VIGE) been successfully employed in model plants, such as Arabidopsis thaliana and Nicotiana spp. In this study, we developed two VIGE for Solanaceous plants. First, used...
Plant breeding explores genetic diversity in useful traits to develop new, high-yielding, and improved cultivars. Ethyl methane sulfonate (EMS) is a chemical widely used induce mutations at loci that regulate economically essential traits. Additionally, it can knock out genes, facilitating efforts elucidate gene functions through the analysis of mutant phenotypes. Here, we developed population using small pungent ornamental Capsicum annuum pepper “Micro-Pep”. This accession particularly...
Pepper (Capsicum spp.) holds significant value as both a vegetable and spice crop cultivated across the world, belonging to genus Capsicum of Solanaceae family. In addition its culinary applications, pepper finds extensive utility in pharmaceutical cosmetics sectors. Breeding plants with improved nutritional qualities will require comprehensive understanding biosynthetic pathway genes their regulatory functions. Advances molecular biology biotechnology have facilitated identification...
Abstract Key message The pepper mutants (‘221-2-1a’ and ‘1559-1-2h’) with very low pungency were genetically characterized. Pun4 locus, responsible for the reduced of mutant fruits, was localized to a 208 Mb region on chromosome 6. DEMF06G16460, encoding 3-ketoacyl-CoA synthase, proposed as strong candidate gene based genetic analyses bulked segregants, DEG, expression analyses. Capsaicinoids are unique alkaloids present in ( Capsicum spp.), synthesized through condensation by-products from...
Pepper (Capsicum spp.) is a vegetable and spice crop in the Solanaceae family with many nutritional benefits for human health. During several decades, horticultural traits, including disease resistance, yield, fruit quality, have been improved through conventional breeding methods. Nevertheless, cultivar development time-consuming process because of long generation time pepper. Recently, speed has introduced as solution shorting cycle long-day or day-neutral field crops, but there only few...
Several genes regulating capsaicinoid biosynthesis including Pun1 (also known as CS ), Pun3 , pAMT and CaKR1 have been studied. However, the gene encoded by Pun2 in non-pungent Capsicum chacoense is unknown. This study aimed to identify genetic mapping using interspecific ( C. × annuum ) intraspecific populations. QTL F 2 population revealed two major QTLs on chromosomes 3 9. Two bin markers within regions were highly correlated with content population. The QTL, Pun2_PJ_Gibbs_3.11 chromosome...
Fruit color is one of the most important traits in peppers due to its esthetic value and nutritional benefits determined by carotenoid composition, resulting from diverse mutations biosynthetic genes. The EMS204 line, derived an EMS mutant population, presents bright-red color, compared with wild type Yuwolcho cultivar. HPLC analysis indicates that fruit contains more zeaxanthin less capsanthin capsorubin than Yuwolcho. MutMap was used reveal variation using F