A5022884001- Mosquito-borne diseases and control
- Malaria Research and Control
- Viral Infections and Vectors
- Virus-based gene therapy research
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Genetics and Neurodevelopmental Disorders
- Lysosomal Storage Disorders Research
- Zoonotic diseases and public health
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- RNA Interference and Gene Delivery
- Iron Metabolism and Disorders
Genethon (France)
2023
Inserm
2023
Université Paris-Saclay
2023
bioMérieux (France)
2012-2015
Fondation Mérieux
2014
A key step for broad viral detection using high-throughput sequencing (HTS) is optimizing the sample preparation strategy extracting viral-specific nucleic acids since genomes are diverse: They can be single-stranded or double-stranded RNA DNA, and vary from a few thousand bases to over millions of bases, which might introduce biases during acid extraction. In addition, particles enveloped non-enveloped with variable resistance pre-treatment, may influence their susceptibility extraction...
Glycogen storage disease type III (GSDIII) is a rare inborn error of metabolism affecting liver, skeletal muscle, and heart due to mutations the AGL gene encoding for glycogen debranching enzyme (GDE). No curative treatment exists GSDIII. The 4.6 kb GDE cDNA represents major technical challenge toward development single recombinant adeno-associated virus (rAAV)-derived vector therapy strategy. Using information on structure molecular modeling, we generated multiple truncated GDEs retaining...
Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic dengue fever or shock syndrome. Prognostication severity urgently required improve patient management. The pathogenesis severe has not been fully elucidated, role host proteins associated with particles received little exploration. proteomes virion-enriched fractions purified from plasma pools patients were...
Abstract Dengue virus (DV) infection is the most common mosquito‐born viral disease of public health significance. Though patients only suffer from flu‐like symptoms, a small group experiences more severe forms disease. The nonstructural protein 1 (NS1), secreted correlating with viremia, key element used for dengue diagnosis potential implications in prognosis. Capture‐ELISAs early detection NS1 sera during acute febrile stage are commonly routine by diagnostic laboratories. In this study,...
Dengue fever is the most important arthropod born viral disease of public health significance. Although patients suffer only from flu-like symptoms, a small group patient experiences more severe forms disease. To contribute to better understanding its pathogenesis this study aims identify proteins differentially expressed in pool five viremic plasma dengue relative non-severe patients.The use Isotope Coded Protein Labeling (ICPLTM) analyze depleted twenty high-abundance allowed for...
Limb-Girdle Muscular Dystrophy R9 (LGMDR9) is a dystroglycanopathy caused by Fukutin-related protein (FKRP) defects leading to the deficiency of α-DG glycosylation, essential membrane integrity. Recombinant adeno-associated viral vector (rAAV) gene therapy offers great therapeutic promise for such neuromuscular disorders. Pre-clinical studies have paved way phase 1/2 clinical trial aiming evaluate safety and efficacy FKRP in LGMDR9 patients. To demonstrate product activity, quality,...
Dengue is an endemic viral disease present in inter-tropical countries. If dengue usually benign, more severe forms (severe [SD]) may lead to serious complications. The prognosis of SD currently unreliable. To improve the prognosis, it could be necessary know key elements pathogenicity SD. Many hypotheses have been developed explain a higher patients. Numerous studies highlighted role host immune response and infecting virus strain. development these hypothesis allows better understanding...