A5026321149- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Origins and Evolution of Life
- Health, Environment, Cognitive Aging
Universität Greifswald
2014-2024
Universitätsmedizin Greifswald
2016-2023
<ns4:p>Since the discovery of first catalytic RNA in 1981, field ribozyme research has developed from motifs nature and elucidation their structures mechanisms, into a engineering design towards application diagnostics, molecular biology medicine. Owing to development powerful protocols for selection nucleic acid catalysts with desired functionality random libraries, spectrum supported reactions greatly enlarged, importantly, ribozymes have been accompanied by DNAzymes. Current areas are...
Abstract Binding of an indoloquinoline derivative with aminoalkyl side chain to a truncated sequence from the MYC promoter region was studied through isothermal titration calorimetry (ITC). The targeted MYC3 lacks 3′‐flanking nucleotides and forms monomeric parallel quadruplex (G4) blunt‐ended 3′‐outer tetrad under solution conditions employed. Analysis ITC isotherms reveals multiple binding equilibria initial formation 1:2 ligand/quadruplex complex. Evaluation electrophoretic mobilities as...
We have engineered a hairpin ribozyme that in two-step reaction supports RNA recombination by catalysing the cleavage of two non-functional precursors (first step) followed fragments into functional (second step). The product (yield: 76%) is fully hammerhead ribozyme.
In the transition from RNA world to modern DNA/protein world, RNA-catalyzed aminoacylation might have been a key step towards early translation. A number of ribozymes capable aminoacylating their own 3' termini developed by in vitro selection. However, all those catalysts require previously activated amino acid-typically an aminoacyl-AMP-as substrate. Here we present two connected intermolecular base pairing and carrying out steps aminoacylation: ribozyme 1 loads nonactivated phenylalanine...
Abstract RNA repair is an emerging strategy for gene therapy. Conventional therapy typically relies on the addition of corrected DNA sequence a defective to restore function. As additional option, allows alteration endogenous messenger RNAs (mRNAs). mRNA either facilitated by intracellular spliceosome machinery or intrinsic catalytic activity trans ‐acting ribozymes. Previously we developed twin ribozymes, derived from hairpin ribozyme, tandem duplication and demonstrated their potential...
Twin ribozymes mediate the exchange of a short patch RNA against an exogenous oligonucleotide within suitable substrate. Thus, twin are promising tools for repair, i.e. treatment genetic disorders at mRNA level. A number ribozyme-mediated fragment reactions have been successfully demonstrated using model substrates. Herein we show first time in vitro repair full-length transcript and translation into functional protein. The system is based on designed mutant EGFP containing four-base...
Conducting large-scale epidemiologic studies requires powerful software for electronic data capture, management, quality assessments, and participant management. There is also an increasing need to make the collected findable, accessible, interoperable, reusable (FAIR). However, tools from major studies, underlying such needs, are not necessarily known other researchers. Therefore, this work gives overview on main used conduct internationally highly networked population-based project Study...
Abstract Wir berichten über das Design einer RNA‐Sequenz, die zwei verschiedene Ribozym‐Faltungen annehmen und Spaltung und/oder Ligation der jeweiligen Substrate katalysieren kann. Diese RNA ist in Lage, ihre Konformation als Reaktion auf Umgebung zu ändern, weshalb wir sie Chamäleon‐Ribozym (CHR) bezeichnen. Die effiziente RNA‐Spaltung von verschiedenen Substraten sowie RNA‐Ligation durch CHR wurde separaten Experimenten Ein‐Topf‐Reaktion nachgewiesen. Unsere Studie zeigt, dass sich...
We report the design of a single RNA sequence capable adopting one two ribozyme folds and catalyzing cleavage and/or ligation respective substrates. The is able to change its conformation in response environment, hence it called chameleon (CHR). Efficient different substrates as well by CHR demonstrated separate experiments pot reaction. Our study shows that variants hairpin intersect with hammerhead rather short molecules can have comprehensive conformational flexibility, which an important...