A5018573477- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced NMR Techniques and Applications
- Atomic and Subatomic Physics Research
- Nuclear Physics and Applications
- Ionic liquids properties and applications
- Electrochemical Analysis and Applications
- NMR spectroscopy and applications
- Metabolomics and Mass Spectrometry Studies
- Molecular Junctions and Nanostructures
- Mesoporous Materials and Catalysis
- Nuclear physics research studies
- Advanced X-ray Imaging Techniques
- Analytical Chemistry and Chromatography
- Particle physics theoretical and experimental studies
- Metal complexes synthesis and properties
- Material Dynamics and Properties
- Laser-induced spectroscopy and plasma
- Quantum, superfluid, helium dynamics
- Molecular spectroscopy and chirality
- Bacteriophages and microbial interactions
- Thermodynamic properties of mixtures
- Polyoxometalates: Synthesis and Applications
University of Geneva
2020-2022
European Organization for Nuclear Research
2021-2022
Universität Greifswald
2015-2019
Universitätsmedizin Greifswald
2018
Abstract The oligomer d(GCGTG 3 TCAG TG ACGC) with short complementary flanking sequences at the 5′‐ and 3′‐ends was shown to fold into three different DNA G‐quadruplex species. In contrast, a corresponding that lacks base complementarity between two overhang folds single parallel G‐quadruplex. coexisting quadruplex structures were unambiguously identified structurally characterized through detailed spectral comparisons well‐defined G‐quadruplexes formed upon deliberate incorporation of syn...
A MYC sequence forming an intramolecular G-quadruplex with a parallel topology was modified by the incorporation of 8-bromoguanosine (BrG) analogues in one its outer G-tetrads. The propensity BrG to adopt syn glycosidic torsion angle results exceptional monomolecular quadruplex conformation featuring complete flip tetrad while keeping orientation all G-tracts as shown circular dichroism and nuclear magnetic resonance spectroscopic studies. When substituting three four G-tetrad residues...
Abstract Binding of an indoloquinoline derivative with aminoalkyl side chain to a truncated sequence from the MYC promoter region was studied through isothermal titration calorimetry (ITC). The targeted MYC3 lacks 3′‐flanking nucleotides and forms monomeric parallel quadruplex (G4) blunt‐ended 3′‐outer tetrad under solution conditions employed. Analysis ITC isotherms reveals multiple binding equilibria initial formation 1:2 ligand/quadruplex complex. Evaluation electrophoretic mobilities as...
The interactions of a dicarbocyanine dye 3,3'-diethylthiadicarbocyanine, DiSC2(5), with DNA G-quadruplexes were studied by means combination various spectroscopic techniques. Aggregation excess as result its positive charge is promoted the presence polyanionic quadruplex structure. Specific high-affinity binding to parallel MYC promoter sequence involves stacking DiSC2(5) on external G-tetrads; 5'-terminal tetrad favored site. Significant energy transfer between and in UV spectral region...
Over the last few years, Deep Eutectic Solvents have gained popularity as a novel class of green solvents, due to their feasible synthesis and overall low production costs. The properties glycerol (Gly)-based are frequently associated with formation an extended hydrogen bond network. In this study, two-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy is employed analyse effect oversaturation acceptor, choline chloride (ChCl) on structural arrangement glyceline (molar ratio 1 : 2...
A benzothiazole derivative was identified as potent ligand for DNA G-quadruplex structures. Fluorescence titrations revealed selective binding to quadruplexes of different topologies including parallel, antiparallel, and (3+1) hybrid The parallel c-MYC sequence found constitute the preferred target with dissociation constants in micromolar range. Binding benzothiazole-based structurally thermodynamically characterized detail by employing a comprehensive set spectroscopic calorimetric...
Imidazolium-based ionic liquids (ILs) are frequently used solvents, due to their peculiar structure resulting in low melting points. Recent studies revealed the direct impact of water molecules on self-structuring IL nano-domains. Therefore, this work influence different impurities network and its vacuum stability has been studied using Nuclear Magnetic Resonance (NMR) Electrochemical Impedance Spectroscopy (EIS). As a result, three distinguishable interdependent regions could be identified....
Charge transfer across double stranded DNA was observed for the first time about 20 years ago, and ever since it has been subject of a large number studies. RNA hardly investigated in this regard, which not least is due to lack suitably functionalized ribonucleotide building blocks serve as electron sources upon incorporation into oligoribonucleotides. We have synthesized two uridine derivatives carrying pyrene or dimethylaminopyrene linked C5 nucleobase. The key successful synthesis...
The T1 relaxation time measured in nuclear magnetic resonance experiments contains information about electric field gradient (EFG) fluctuations around a nucleus, but computer simulations are typically required to interpret the underlying dynamics. This study uses classical molecular dynamics (MD) and quantum chemical calculations, investigate EFG Na+ ion dissolved ionic liquid 1-ethyl 3-methylimidazolium tetrafluoroborate, [Im21][BF4], provide framework for future interpretation of NMR...
A G-quadruplex forming sequence from the MYC promoter region was modified with syn-favoring 8-bromo-2'-deoxyguanosine residues. Depending on number and position of modifications in intramolecular parallel G-quadruplex, substitutions bromoguanosine analogue at 5'-tetrad induce conformational rearrangements concerted all-anti to all-syn transitions for all residues G-quartet. No unfavorable steric interactions C8-substituents medium grooves are apparent high-resolution structure as determined...
Abstract Es konnte gezeigt werden, dass sich ein DNA‐Oligonukleotid der Sequenz d(GCGTG 3 TCAG TG ACGC) mit kurzen, komplementären Überhangsequenzen am 5′‐ und 3′‐Ende in drei unterschiedliche Quadruplexspezies faltet. Dagegen bildet das entsprechende Oligomer ohne Basenkomplementarität beiden nur eine einzige parallele G‐Quadruplexstruktur. Über einen Vergleich von NMR‐Spektren polymorphen Probe genau definierten G‐Quadruplexen, die über gezielten Einbau syn‐präferierenden...
Recently there has been an increased interest to apply the sensitive β-decay asymmetry detected nuclear magnetic resonance (β-NMR) technique biological studies. A liquid-sample β-NMR setup was build at ISOLDE allow such investigations and use resolution gain of liquid-state NMR in physics. As part this a field locking system, set printed circuit board shimming coils, sample exchange compact β-detectors custom experimental vacuum chamber were developed. The main stabilized down ppm level by...
The front cover artwork is provided by Patryk Palenque Marcinkowski. image shows a glycerol-choline network accommodating water molecules over time. glycerol are represented as pandas adapting to the change. Read full text of Research Article at 10.1002/cphc.202100806.
We determine for the first time magnetic dipole moment of a short-lived nucleus with part-per-million (ppm) accuracy. To achieve this two orders magnitude improvement over previous studies, we implement number innovations into our $\beta$-detected Nuclear Magnetic Resonance ($\beta$-NMR) setup at ISOLDE/CERN. Using liquid samples as hosts obtain narrow, sub-kHz linewidth, resonances, while simultaneous in-situ $^1$H NMR measurement allows us to calibrate and stabilize field ppm precision,...
$\beta$-NMR is an advancing technique that enables measurements relevant to various fields of research, ranging from physics chemistry and biology. Among the recent achievements setup located at ISOLDE facility CERN determination magnetic moment a shortlived nucleus with part-per-million accuracy. Presented here are major upgrades extensions setup. The most important advancement installation 4.7 T superconducting solenoidal magnet, leading sub-ppm spatial homogeneity temporal stability...
The global need to expand the design of energy-storage devices led investigation alkali metal - Ionic Liquid (IL) mixtures as a possible class electrolytes. In this study, 1D and 2D Nuclear Magnetic Resonance (NMR) Electrochemical Impedance Spectroscopy (EIS) well Molecular Dynamics (MD) simulations were used study intermolecular interactions in imidazolium-based IL water halide ternary mixtures. 1 H 23 Na DOSY NMR spectra revealed that presence small quantities NaCl does not influence...