A5054473457- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Advanced NMR Techniques and Applications
- RNA Interference and Gene Delivery
- Electron Spin Resonance Studies
- Lipid Membrane Structure and Behavior
- Molecular Junctions and Nanostructures
- Protein Structure and Dynamics
- Atomic and Subatomic Physics Research
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2023
Universität Greifswald
2019-2021
Abstract The oligomer d(GCGTG 3 TCAG TG ACGC) with short complementary flanking sequences at the 5′‐ and 3′‐ends was shown to fold into three different DNA G‐quadruplex species. In contrast, a corresponding that lacks base complementarity between two overhang folds single parallel G‐quadruplex. coexisting quadruplex structures were unambiguously identified structurally characterized through detailed spectral comparisons well‐defined G‐quadruplexes formed upon deliberate incorporation of syn...
Abstract A parallel quadruplex derived from the Myc promoter sequence was extended by a stem‐loop duplex at either its 5′‐ or 3′‐terminus to mimic quadruplex–duplex (Q–D) junction as potential genomic target. High‐resolution structures of hybrids demonstrate continuous stacking on core without significant perturbations. An indoloquinoline ligand carrying an aminoalkyl side chain shown bind Q–D with very high affinity in order K ≈10 7 m −1 irrespective location 3′‐ 5′‐end. NMR chemical shift...
Abstract Canonical G‐quadruplexes can adopt a variety of different topologies depending on the arrangement propeller, lateral, or diagonal loops connecting four G‐columns. A novel intramolecular G‐quadruplex structure is derived through inversion last G‐tract three‐layered parallel fold, associated with transition single propeller into lateral loop. The resulting (3+1) hybrid fold features three syn⋅anti⋅anti⋅anti G‐tetrads 3’‐terminal all‐ syn G‐column. Although ability forming duplex...
Viroporins are small ion channels in membranes of enveloped viruses that play key roles during viral life cycles. To use viroporins as drug targets against infection requires in-depth mechanistic understanding and, with that, methods enable investigations under situ conditions. Here, we apply surface-enhanced infrared absorption (SEIRA) spectroscopy to Influenza A M2 reconstituted within a solid-supported membrane, shed light on the mechanics its viroporin function. is paradigm pH-activated...
Abstract Es konnte gezeigt werden, dass sich ein DNA‐Oligonukleotid der Sequenz d(GCGTG 3 TCAG TG ACGC) mit kurzen, komplementären Überhangsequenzen am 5′‐ und 3′‐Ende in drei unterschiedliche Quadruplexspezies faltet. Dagegen bildet das entsprechende Oligomer ohne Basenkomplementarität beiden nur eine einzige parallele G‐Quadruplexstruktur. Über einen Vergleich von NMR‐Spektren polymorphen Probe genau definierten G‐Quadruplexen, die über gezielten Einbau syn‐präferierenden...
Abstract Viroporins are small ion channels in membranes of enveloped viruses that play key roles during viral life cycles. To use viroporins as drug targets against infection requires in-depth mechanistic understanding and, with that, methods enable investigations under in-situ conditions. Here, we apply surface-enhanced infrared absorption (SEIRA) spectroscopy to Influenza A M2 reconstituted within a solid-supported membrane, shed light on the mechanics its viroporin function. is paradigm...
Abstract Viroporine sind kleine Ionenkanäle in Membranen umhüllter Viren, die Schlüsselrollen viralen Lebenszyklen spielen. Um als Wirkstoffziele zur Bekämpfung von Virusinfektionen nutzen zu können, bedarf es eines umfassenden Verständnisses ihrer Funktionsweise. Hierfür wiederum Methoden notwendig, ihre Untersuchung unter situ‐Bedingungen ermöglichen. In dieser Arbeit wir oberflächenverstärkte Infrarotabsorptionsspektroskopie (SEIRA, engl. surface‐enhanced infrared absorption ), um den...