A5068288385- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Hepatitis C virus research
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Liver Disease Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Hepatitis B Virus Studies
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2023
National Agricultural Technology Institute
2012-2022
National Institute of Industrial Technology
2017-2019
Instituto de Genética Veterinaria
2010
University of Milan
1999
ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in respiratory tract for up to 72 h postinfection, followed hematogenous dissemination vesicular lesions at oral foot epithelia. The role of early local adaptive immunity host outcome infection not well understood. Here we report kinetics appearance FMDV-specific...
Foot-and-mouth disease virus (FMDV) causes a highly contagious in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies B-epitope [VP1(136–154)] linked to T-cell epitope [3A(21–35)] FMDV confers protection type O challenge pigs. Herein we show cattle that novel bearing [VP1(21–40] and or four B-cell [VP1(135–160)] from O1 Campos (termed B2T B4T, respectively) elicited specific immune responses similar levels commercial vaccine. Animals...
ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied generation of antibody responses in local lymphoid tissues along respiratory system vaccinated and further aerosol-infected cattle. Animals immunized high-payload monovalent vaccine developed high titers neutralizing antibodies at 7 days postvaccination (dpv),...
This study investigated the induction of humoral and cellular immune response by a DNA vaccine based on bovine herpesvirus-1 (BoHV-1) glycoprotein D with commercial adjuvants (SEPPIC), in murine model preliminary assay cattle, order to select vaccines candidates that can improve response. A most used this was able elicit gD viral-specific vaccinated mice. Nevertheless, only Montanide GEL 01 PR Essai 903110 induced proliferation highest levels IFN-γ secretion. Since is important deal BoHV-1...
Foot-and-mouth disease (FMD) remains one of the major threats to animal health worldwide. Its causative agent, FMD virus (FMDV), affects cloven-hoofed animals, including farm animals and wildlife species, inflicting severe damage international trade livestock industry. FMDV antigenic variability biggest challenges for vaccine-based control strategies. The current study analyzed host's adaptive immune responses in cattle immunized with different vaccine protocols investigated its associations...
Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear showed that they failed to induce solid protection in cattle. Dendrimeric displaying two or four copies a peptide corresponding the B-cell epitope VP1 [136–154] type O FMDV (O/UKG/11/2001) linked through thioether bonds single copy 3A [21–35] (termed B 2 T 4 T, resp.) afforded vaccinated pigs. In this work, we...
Dendritic cells (DC) are antigen-presenting uniquely capable of priming naïve T and cross-presenting antigens, they determine the type immune response elicited against an antigen. TAT peptide (TATp), is amphipathic, arginine-rich, cationic that promotes penetration translocation various molecules nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer anchoring in liposomal membrane. Here, we show...
Immunosuppressive diseases cause great losses in the poultry industry, increasing susceptibility to infections by other pathogens and promoting a suboptimal response vaccination. Among them, infectious bursal disease virus (IBDV) arises as one of most important around world. IBDV infects immature B lymphocytes, affecting immune status birds facilitating such avian bronchitis (IBV). Although it has been reported that interaction between these viruses increases IBV clinical signs, there are no...
Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection cattle has been largely correlated with the FMD virus (FMDV)-specific antibodies. Genetic of immune adaptive responses demonstrated only for peptide antigens derived from FMDV structural proteins. Here, we quantify heterogeneity antibody response primo-vaccinated against study its association genetic background...
Twelve children born to hepatitis C virus antibody GBV-C/HGV RNA positive mothers who acquired infection by the vertical or perinatal route were studied. Most (91%) persistently up 12 months of age. Four out six cases alone had normal alanine amino transferase activities. Long lasting evidence hepatocellular injury was detected only in with and HIV coinfection.
Little is known about the influence of maternal antibodies and immune cells transferred through colostrum on responses calves to currently used foot-and-mouth disease (FMD) vaccines. Here we evaluated humoral cellular induced by vaccination colostrum-deprived that received equivalent amounts preparations differed in presence or absence but contained same quantity quality anti-foot-and-mouth virus (FMDV) antibodies. Three groups 32-d-old (n = 3 per group) were deprived fed either whole a...