A5044095482- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- T-cell and Retrovirus Studies
- Immunotherapy and Immune Responses
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Mycobacterium research and diagnosis
- Nitric Oxide and Endothelin Effects
- Parasitic Infections and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- S100 Proteins and Annexins
- Vector-borne infectious diseases
- Hepatitis B Virus Studies
- Invertebrate Immune Response Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Ginseng Biological Effects and Applications
- Receptor Mechanisms and Signaling
- Wnt/β-catenin signaling in development and cancer
- Flame retardant materials and properties
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2025
Hospital Muñiz
2025
National Institute of Industrial Technology
2012-2021
National Agricultural Technology Institute
2005-2021
Universidad del Salvador
2010-2021
Instituto Nacional de Tecnologia
2020
Austral University of Chile
2012-2019
Centro Científico Tecnológico - San Juan
2014
Pontificia Universidad Católica de Chile
2012
Texas A&M Health Science Center
2012
Endothelial adherens junction proteins constitute an important element in the control of microvascular permeability. Platelet-activating factor (PAF) increases permeability to macromolecules via translocation endothelial nitric oxide synthase (eNOS) cytosol and stimulation eNOS-derived signaling cascade. The mechanisms by which regulates at junctions are still incompletely understood.We explored hypothesis that PAF stimulates hyperpermeability S-nitrosation (SNO) proteins.We measured...
Bovine herpesvirus type 1 (BoHV-1) is the causative agent of respiratory and genital tract infections; causing a high economic loss in all continents. Use marker vaccines IBR eradication programs widely accepted since it allows for protection animals against disease while adding possibility differentiating vaccinated from infected animals. The aim present study was development evaluation safety efficacy glycoprotein E-deleted (gE-) BoHV-1 vaccine strain (BoHV-1ΔgEβgal) generated by...
Summary Paratuberculosis (Ptbc) has a high prevalence in Argentina, that affects dairy and beef cattle. The culture is the gold standard to diagnosis of disease. Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis) , aetiological agent, difficult isolate grow culture. In this study, 24 randomly selected cows Fresian breed from herd with history Ptbc were used evaluate performance different diagnostic techniques. These animals did not show clinical signs However, another animal...
Galectin-8 (Gal-8) is a mammalian β-galactoside-binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen-specific immune responses in vivo, we investigated whether Gal-8 was also able promote APC activation sustain T cell after priming. Both endogenous [dendritic cells (DCs)] and bone marrow-derived DCs (BMDCs) treated exogenous exhibited mature phenotype characterized by increased MHC class II (MHCII), CD80, CD86 surface expression. Moreover,...
The adherens junction complex, composed mainly of vascular endothelial (VE)-cadherin, β-catenin, p120, and γ-catenin, is the main element barrier in postcapillary venules. S-nitrosylation β-catenin p120 an important step proinflammatory agents-induced hyperpermeability. We investigated vitro vivo whether or not VE-cadherin S-nitrosylated using platelet-activating factor (PAF) as agonist. report that PAF-stimulates VE-cadherin, which disrupts its association with β-catenin. In addition, based...
Foot-and-mouth disease virus (FMDV) causes a highly contagious in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies B-epitope [VP1(136–154)] linked to T-cell epitope [3A(21–35)] FMDV confers protection type O challenge pigs. Herein we show cattle that novel bearing [VP1(21–40] and or four B-cell [VP1(135–160)] from O1 Campos (termed B2T B4T, respectively) elicited specific immune responses similar levels commercial vaccine. Animals...
The permeability of endothelial cells is regulated by the stability adherens junctions, which highly sensitive to kinase-mediated phosphorylation and nitric oxide synthase (eNOS)-mediated S-nitrosylation its protein components. Solid tumors can produce a variety factors that stimulate these signaling pathways leading cell hyperpermeability. This generates stromal conditions facilitate tumoral growth dissemination. Galectin-8 (Gal-8) overexpressed in several carcinomas has cellular effects...
The study assessed the effect of soil slope on Mycobacterium avium subsp. paratuberculosis transport into rainwater runoff from agricultural after application M. paratuberculosis-contaminated slurry. Under field conditions, 24 plots undisturbed loamy 1 by 2 m(2) were placed platforms. Twelve used for water runoff: 6 at a 3% and 15% slope. Half each treated with slurry, half not treated. Using same experimental design, 12 established sampling monthly basis using spiked slurry slopes. Runoff...
Bovine herpesvirus-1 (BoHV-1) is the causative agent of bovine infectious rhinotracheitis, an important disease worldwide. Although conventional BoHV-1 vaccines, including those based on use modified live virus and also inactivated are currently used in many countries, they have several disadvantages. DNA vaccines emerged as attractive approach since potential to induce both humoral cellular immune response; nevertheless, it largely known that potency naked limited. We demonstrated...
This study investigated the induction of humoral and cellular immune response by a DNA vaccine based on bovine herpesvirus-1 (BoHV-1) glycoprotein D with commercial adjuvants (SEPPIC), in murine model preliminary assay cattle, order to select vaccines candidates that can improve response. A most used this was able elicit gD viral-specific vaccinated mice. Nevertheless, only Montanide GEL 01 PR Essai 903110 induced proliferation highest levels IFN-γ secretion. Since is important deal BoHV-1...
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, simple, efficient, and low-cost method harvesting DNA from Map cultured liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) evaluated by comparing its performance to that two traditional techniques (a QIAamp Stool Mini Kit cethyltrimethylammonium bromide [CTAB] method). results were statistically assessed...
We tested the hypothesis that platelet-activating factor (PAF) induces S-nitrosylation of vasodilator-stimulated phosphoprotein (VASP) as a mechanism to reduce microvascular endothelial barrier integrity and stimulate hyperpermeability. PAF elevated VASP above baseline levels in different cells caused To ascertain importance nitric oxide synthase (eNOS) subcellular location this process, we used ECV-304 transfected with cytosolic eNOS (GFPeNOSG2A) plasma membrane (GFPeNOSCAAX). induced but...
Glioblastoma is a highly aggressive brain tumor, characterized by the formation of dysfunctional blood vessels and permeable endothelial barrier. S-nitrosylation, posttranslational modification, has been identified as regulator function. In this work we explored whether S-nitrosylation induced glioblastoma tumors regulates As proof concept, observed that present in tumoral microenvironment two different animal models. Subsequently, measured S nitrosylation microvascular permeability EAhy296...