A5007759747- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Urinary Tract Infections Management
- Nail Diseases and Treatments
- Genital Health and Disease
- Pelvic floor disorders treatments
- Cervical Cancer and HPV Research
- Cutaneous lymphoproliferative disorders research
- Viral Infections and Outbreaks Research
- Bacterial Infections and Vaccines
- Virology and Viral Diseases
- Dermatology and Skin Diseases
- Viral Infections and Vectors
- Urologic and reproductive health conditions
- Autoimmune Bullous Skin Diseases
- Animal Disease Management and Epidemiology
- Urological Disorders and Treatments
- Adolescent Sexual and Reproductive Health
- Infectious Diseases and Mycology
- Virus-based gene therapy research
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
- Sex work and related issues
- Viral Infections and Immunology Research
- Urinary and Genital Oncology Studies
University of Bologna
2012-2022
Policlinico S.Orsola-Malpighi
2011-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2006-2020
National Food Safety and Quality Service
2019
Hospital César Milstein
2008-2017
University of Washington
2017
Università Cattolica del Sacro Cuore
2016
Istituto Superiore di Sanità
2008
University of Buenos Aires
2001
Institute of Dermatology
1997
Abstract We characterized the vaginal ecosystem during common infections of female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, 20), recruiting healthy (HC, 21) bacterial vaginosis-affected (BV, 20) women references eubiosis dysbiosis. The profiles microbiome metabolome were studied in 79 reproductive-aged women, by means next generation sequencing proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly...
The vaginal microbiota plays a crucial role in maintaining the health and functioning of female genital tract, preventing colonization urogenital pathogens sexually transmitted infections. In this study, we characterized bacterial communities metabolome associated to Chlamydia trachomatis infection (CT: 20 women), compared healthy condition (H: 22 women) vaginosis (BV: 19 women). A microarray-based tool (VaginArray), implemented with real-time PCR for Gardnerella vaginalis, was used...
Abstract Background Limited data are available on risk factors associated with lichen sclerosus and no gender differences in genital ( GLS ). Objective This multicentre study aimed at identifying potential for , through collection from a large, mixed‐sex sample of patients comparing gender‐related relation to the general population. Methods was cross‐sectional 729 subjects (53.8% females, 46.2% males) affected consecutively observed within network 15 Italian dermatology units. The following...
Syphilitic alopecia (SA) is mainly described in single case reports, and there are only a few epidemiological studies.To investigate the clinical dermoscopic features of SA patients with secondary syphilis to record its actual prevalence.All diagnosis were clinically trichoscopically evaluated search hair scalp alterations.Five 12 (41.6%) diagnosed had SA. The 'moth-eaten' pattern was detected 4 (80% cases): 1 'essential' SA, whereas 3 'symptomatic' 'diffuse' found patient. Trichoscopy areas...
We report six HIV patients who developed painful periungual inflammation of several nails during treatment with the antiretroviral drugs indinavir and lamivudine. The lesions appeared 2–12 months after starting treatment. occurrence paronychia in has recently been reported two groups receiving either or Dermatologists should be aware this probably not uncommon side‐effect order to avoid an invasive approach nail lesions.
ABSTRACT The purpose of this study was to evaluate the diagnostic performance LIAISON Treponema Screen (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with that rapid plasma reagin (RPR) and following currently used treponemal tests: hemagglutination test (TPHA), immunoenzymatic assay (EIA), Western blot (WB). First, retrospective performed panel 2,494 blood donor sera, 131 clinical serologically characterized syphilitic 96 samples obtained...
Tacaribe virus (TCRV) belongs to the Arenaviridae family. Its bisegmented negative-stranded RNA genome encodes nucleoprotein (N), precursor of envelope glycoproteins, polymerase (L), and a RING finger matrix (Z) protein. The 570-amino-acid N protein binds viral RNA, forming nucleocapsids, which are template for transcription replication by polymerase. We have previously shown that interaction between Z is required assembly infectious virus-like particles (VLPs) (J. C. Casabona et al., J....
ABSTRACT Despite recent technological advances, the diagnosis of syphilis remains a challenging enterprise. Actually, most high-volume laboratories have adopted “reverse algorithm” due several factors, including potential to automate testing. Recently, immunoassays processed on random-access systems been proposed as screening tests. The purpose this study was evaluate diagnostic performances BioPlex 2200 Syphilis IgG and IgM, tests based Multiplex Flow technology, in comparison with...
Background Although syphilis rates have been relatively high in Italy for more than 15 years, no data on the molecular types of Treponema pallidum subspecies circulating this country are yet available. Likewise, how widespread is resistance to macrolide or tetracycline antibiotics these strains exist. Such would, however, promote comprehensive studies epidemiology infections and inform future interventions aiming at control other European countries. Goals Study Design Swabs from oral,...
Galectin-8 (Gal-8) is a mammalian β-galactoside-binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen-specific immune responses in vivo, we investigated whether Gal-8 was also able promote APC activation sustain T cell after priming. Both endogenous [dendritic cells (DCs)] and bone marrow-derived DCs (BMDCs) treated exogenous exhibited mature phenotype characterized by increased MHC class II (MHCII), CD80, CD86 surface expression. Moreover,...
We assessed the prevalence and predictors of Chlamydia trachomatis, Neisseriagonorrhoeae Mycoplasmagenitalium rectal infections in a population 'men having sex with men' (MSM).From January to November 2017, 165 MSM attending STI outpatients clinic Bologna (Italy) reporting unsafe anal intercourses were enrolled. An ano-rectal swab was collected from each patient: chlamydial gonococcal diagnosed by commercial NAAT, whereas an in-house quantitative PCR used for M. genitalium detection. In...
The aim of this study was to characterize the urine metabolome women with Chlamydia trachomatis (CT) uro-genital infection (n = 21), comparing it a group CT-negative subjects 98). By means proton-based nuclear magnetic resonance (1H-NMR) spectroscopy, we detected and quantified metabolites cohort 119 pre-menopausal Caucasian women, attending STI Outpatients Clinic in Italy. In case CT positive result, molecular genotyping performed by omp1 gene semi-nested PCR followed RFLP analysis. We were...
Liquid chromatography with electrochemical detector (LC−ED), using a chemically modified electrode coated metalloporphyrin film, is reported for determination of bisphenol A (BPA) migration from polycarbonate baby bottles. The extraction process the samples was performed according to regulations Southern Common Market (MERCOSUR), where certain food-simulating liquids [(A) distilled water, (B) acetic acid 3% V/V in and (C) ethanol 15% water] are defined along controlled time temperature...
Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, 450 sera (200 from blood donors, 200 syphilis patients, 50 potentially cross-reactive) tested to evaluate the diagnostic performance of Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) T. hemagglutination (MHA-TP) for laboratory diagnosis syphilis. None serum specimens donors or potential...
The arenavirus Z is a zinc-binding RING protein that has been implicated in multiple functions during the viral life cycle. These roles of involve interactions with and cellular proteins remain incompletely understood. In this regard, inhibits RNA transcription replication through direct interaction L polymerase. Here, we defined L-binding domain Tacaribe virus (TCRV) structural requirements mediating homo-oligomerization. By using site-directed mutagenesis, coimmunoprecipitation, functional...
Although HIV infection is often associated with several rheumatic diseases, the coexistence of this retroviral and systemic lupus erythematosus (SLE) extremely uncommon. Generally, HIV-related immunosuppression improves SLE symptoms, antiretroviral therapy may lead to an autoimmune disease flare subsequent increase circulating CD4+ cell number. Two HIV-infected female patients discoid (DLE) diagnosed a few months after highly active initiation, are described. To our knowledge, second case...
to evaluate the prevalence of maternal syphilis at delivery and neonatal infection in an Italian urban area, connection with increased flow immigration.A prospective surveillance study was carried out Bologna, Italy, from November 2000 March 2006. All pregnant women were screened for delivery. Infants born seropositive mothers enrolled a follow-up.During period 19,205 gave birth 19,548 infants. A total 85 The overall seroprevalence 0.44%, but it 4.3% eastern Europe 5.8% Central-South...
A systematic evaluation of the susceptibility all Chlamydia trachomatis urogenital serovars (D through K) to levofloxacin, erythromycin, doxycycline, clarithromycin, and azithromycin was performed. All C. had comparable susceptibilities with respect various antimicrobials tested, thus confirming homogeneous data so far obtained regarding antimicrobial agents.
Virus-like particles (VLPs) are promising vaccine candidates because they represent viral antigens in the authentic conformation of virion and therefore readily recognized by immune system. As VLPs do not contain genetic material safer than attenuated virus vaccines. In this study, herpes simplex type 1 (HSV-1) amplicon vectors were constructed to coexpress rotavirus (RV) structural genes VP2, VP6, VP7 used as platforms launch production RV-like (RVLPs) vector-infected mammalian cells....
Objective: The purpose of our study was to evaluate whether briefs made Dermasilk fabric could be an adjuvant tool in the management vulvar lichen sclerosus (LS). Materials and Methods: A controlled, randomized, double-blind versus placebo conducted, comparing standard cotton patients affected by LS during treatment with clobetasol propionate 0.05% ointment vitamin E moisturizer. For each patient, evaluation objective genital signs subjective symptoms typical recorded before start treatment,...