A5065898364- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Diagnosis and treatment of tuberculosis
- Ginseng Biological Effects and Applications
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Veterinary medicine and infectious diseases
- Animal Disease Management and Epidemiology
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- Yersinia bacterium, plague, ectoparasites research
- RNA and protein synthesis mechanisms
- Fungal Biology and Applications
- Immunodeficiency and Autoimmune Disorders
- Evolution and Genetic Dynamics
- Clostridium difficile and Clostridium perfringens research
- T-cell and B-cell Immunology
- Diphtheria, Corynebacterium, and Tetanus
- Medical and Biological Ozone Research
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2025
National Agricultural Technology Institute
2014-2024
Instituto de Biotecnología y Biología Molecular
2005-2024
Instituto de Agrobiotecnología del Litoral
2024
Centro Científico Tecnológico - Tucumán
2024
Agrobiotechnology Institute
2022
Centro Científico Tecnológico - San Juan
2018-2021
National Institute of Industrial Technology
2013-2018
Universidad Nacional del Sur
2018
University of Buenos Aires
2017
A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay, Great Britain and New Zealand was undertaken to determine their relationships each other taxonomic position within the complex. Isolates 30 cases six species pinniped seven related were compared representative standard strains M. The seal could be distinguished members complex, including recently defined 'Mycobacterium canettii' caprae', on basis host preference phenotypic...
ABSTRACT Two hundred twenty-four Mycobacterium bovis isolates, mainly from South American countries, were typed by spoligotyping, and 41 different spoligotypes identified. A total of 202 M. isolates (90%) grouped into 19 clusters. The largest cluster contained 96 (42.8%) on the basis most frequently observed spoligotype, spoligotype 34. Nineteen humans in Argentina had polymorphic GC-rich repetitive sequence (PGRS) types that represented common found among cattle. All five Uruguay three six...
Summary Paratuberculosis (Ptbc) has a high prevalence in Argentina, that affects dairy and beef cattle. The culture is the gold standard to diagnosis of disease. Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis) , aetiological agent, difficult isolate grow culture. In this study, 24 randomly selected cows Fresian breed from herd with history Ptbc were used evaluate performance different diagnostic techniques. These animals did not show clinical signs However, another animal...
The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture Stonebrink media. However, very slow because the extremely long duplication time bacillus and difficult scarcity bacilli diagnostic samples. This study describes development a single-tube touch-down polymerase chain reaction (PCR) protocol for detection using primers that target IS 6110 element. Spiked water milk as well routine (milk nasal swabs) from bovis–positive cattle were tested....
Summary With the hypothesis that genetic variability of Mycobacterium bovis could influence virulence and immunopathology, five M. strains were selected from an epidemiological study in Argentina on basis their prevalence cattle occurrence other species. We then determined immunopathology evoked by these a well-characterized mouse model progressive pulmonary tuberculosis. The reference strain AN5 was used as control. BALB/c mice infected with this showed 50% survival after 4 months...
Bovine tuberculosis is caused by Mycobacterium bovis, a mycobacterium highly similar to M. that belongs the complex. The main host of bovis cattle but it also affects many other mammalians including humans. Tuberculosis in humans either or clinically hard distinguish. During 2004-2005, samples from 448 patients with diagnosis TB were collected different regions Argentina. PRA technique identified 400 isolates representative patterns mycobacterium. predominant ones complex, avium-M....
In the present study, a nested-PCR system, targeting TbD1 region, involving performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity reactions were assessed with DNA samples extracted from tuberculous non-tuberculous mycobacteria, as well other actinomycetales species directly bovine bubaline terms analytical sensitivity, M. AN5 detected up 1.56 ng 97.6 pg 1.53 reaction...
Diagnosis of the Mycobacterium tuberculosis complex by direct PCR mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious bearing tuberculous-like lesions detected during slaughter. The procedure applied on samples (n=54) obtained adding alpha -casein into thiocyanate extraction mix was positive 70% samples. confirmed identification 23 (100%) that grew culture, 9 (60%) failed to grow plus 6 (37.5%) resulted growth bacterial contaminants. Genotyping IS6110-RFLP...
Summary: Southern blotting, sequence analysis and PCR experiments showed that Mycobacterium bovis BCG lack a 12·7 kb fragment present in the genome of tuberculosis. This region is 337 bp downstream RD2 region, which was previously described as being absent from some M. strains. The should be useful target for test to differentiate tuberculosis bovis. An suggests it represents deletion rather than an insertion removes most mce-3 operon, one four highly related operons may involved cell entry,...
Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The eradication campaign carried out in Argentina has considerably improved the health situation of herds. Here we evaluated strategy detect M. bovis-infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) bulk tank raw milk from dairy farms. We 177 samples with official free certificate (TFC) 80 without certificate, non-tuberculosis-free (NTFC), 10 departments Santa Fe province, Argentina. To...
A novel Mycobacterium bovis antigen was identified from an expression library using sera naturally infected cattle. The Escherichia coli recombinant clone expressed a 27 kDa protein, named P27. rabbit serum against the recognized protein of in cellular extracts M. and tuberculosis. No culture supernatant. Sequence analysis indicated that P27 has molecular mass 24 kDa, showing characteristic signal sequence for lipoprotein modification (a peptidase type II site). gene is identical to...
mce3 is one of the four mce operons in Mycobacterium tuberculosis that encode exported proteins with a probable role virulence this bacterium. Upstream there putative regulatory gene (Rv1963) harbours double tetR-family signature. To study transcriptional regulation operon, smegmatis mc(2)155 and M. H37Rv strains harboured fusions between promoter region Escherichia coli lacZ gene, either containing or not Rv1963 were used. The presence greatly reduced beta-galactosidase activity, suggesting...
Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized mycobacteria detected through cytology 12 7 with generalized from province Buenos Aires Argentina. In dogs, molecular testing confirmed presence Mycobacterium avium subsp. hominissuis (MAH) 8 cases M. fortuitum 1 case. All were Miniature Schnauzers, suggesting this breed may...
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge strain diversity in Argentina. Although MAP has been previously investigated Argentina using IS900-RFLP, a small number were employed, and low discriminative power was reached. The aim present study test genetic among an MLVA approach based on 8 repetitive loci. We studied 97 from cattle, goat sheep could describe 7 different patterns:...
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for IS900 MAP. The limits detection using the loop-mediated amplification (LAMP) protocols described herein were similar those conventional PCR targeting same sequences. Hydroxynaphtol blue SYBR GreenTM allowed direct naked-eye with identical sensitivity as agarose gel...
Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern multi-organ lesions attributable to Mycobacterium bovis (the causative agent bTB) bacteraemia. A case–control study was conducted investigate blood PCR as an alternative tool for improving ante-mortem detection TST false-negative...