A5028366180- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Toxoplasma gondii Research Studies
- T-cell and Retrovirus Studies
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Brucella: diagnosis, epidemiology, treatment
- Amoebic Infections and Treatments
- Macrophage Migration Inhibitory Factor
- Herpesvirus Infections and Treatments
National University of General San Martín
2018-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2018-2025
Universidad Nacional de San Martín
2024
Centro Científico Tecnológico - San Juan
2019
Summary Galectin‐8 (Gal‐8) is a mammalian lectin endowed with the ability to co‐stimulate antigen‐specific immune responses. We have previously demonstrated that bone‐marrow‐derived dendritic cells produce high levels of interleukin‐6 ( IL ‐6) in response Gal‐8 stimulation. As ‐6 pleiotropic cytokine has broad effect on system, we aimed elucidate whether was involved Gal‐8‐dependent co‐stimulatory signals during antigen recognition by specific CD 4 T cells. With this aim, splenocytes from DO...
Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from nature. Both Gal-8 were together internalized localized in early endosomes. Interestingly, processing by APCs was also accelerated presence as separate mechanism, distinct increased internalization. Moreover, pulsed able to activate...
Galectins constitute a family of soluble lectins with unique capacity to induce macroscale rearrangements upon interacting cell membrane glycoconjugates. Galectin-8 (Gal-8) is acknowledged for its role in facilitating antigen uptake and processing engaging surface glycoconjugates on antigen-presenting cells (APCs). Gal-8 consists two covalently fused N- C-terminal carbohydrate recognition domains (N- C-CRD), each exhibiting distinct glycan specificity. In this study, we utilized single C-CRD...
Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of recombinant protein is a recurrent strategy for studying their biological Here, we provide general protocol production isolated or chimeric domains. We take advantage lectin activity 6xHis-tag addition purification, thus obtaining highly pure active Galectin to both vitro vivo assays. For complete details on execution this protocol, please refer...
Although replication-defective human adenovirus type 5 (Ad5) vectors that express in situ the capsid-encoding region of foot-and-mouth disease virus (FMDV) have been proven to be effective as vaccines relevant species for several viral strains, same result was not consistently achieved O1/Campos/Brazil/58 strain. In present study, an optimization Ad5 system explored and enhance expression FMDV capsid proteins their association into virus-like particles (VLPs). Particularly, we engineered a...