A5100695791- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Bone Metabolism and Diseases
- Folate and B Vitamins Research
- Epigenetics and DNA Methylation
- TGF-β signaling in diseases
- Medical and Biological Sciences
- Temporomandibular Joint Disorders
- Medical Education and Admissions
- NF-κB Signaling Pathways
- Prenatal Screening and Diagnostics
- Immune cells in cancer
- Periodontal Regeneration and Treatments
- Medicinal plant effects and applications
- Genomic variations and chromosomal abnormalities
- Curcumin's Biomedical Applications
- IgG4-Related and Inflammatory Diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Veterinary medicine and infectious diseases
- Prenatal Substance Exposure Effects
- Synthesis of heterocyclic compounds
- Pharmacological Effects and Toxicity Studies
Jiangsu Cancer Hospital
2020-2025
Nanjing Medical University
2020-2025
Harvard University
2012-2021
Albert Einstein College of Medicine
2015-2019
Shandong University
2019
Shandong Provincial Hospital
2019
Hubei University of Medicine
2019
Center for Drug Evaluation and Research
2018
United States Food and Drug Administration
2018
Second Affiliated Hospital of Zhejiang University
2017
Curcumin has been shown to have chondroprotective potential in vitro. However, its effect on disease and symptom modification osteoarthritis (OA) is largely unknown. This study aimed determine whether curcumin could slow progression of OA relieve OA-related pain a mouse model destabilization the medial meniscus (DMM). Expression selected cartilage degradative-associated genes was evaluated human primary chondrocytes treated with nanoparticles assayed by real-time PCR. The mice subjected DMM...
Interleukin-8 (IL-8) plays a vital role in the invasion and metastasis of hepatocellular carcinoma (HCC), is closely associated with poor prognosis HCC patients. Integrin αvβ3, member integrin family, has been reported to be overexpressed cancer tissues mediate cells. However, relationship between IL-8 αvβ3 underlying mechanism remains unclear.The expression IL-8, αv β3 cells was detected by quantitative real-time PCR, Western blot immunohistochemistry. Transwell assay used detect...
Abstract Objective To examine the pathogenetic mechanisms of osteoarthritis (OA)–like changes in Col9a1 −/− mice, which are deficient type IX collagen. Methods Knee joints and temporomandibular (TMJs) from mice their wild‐type (Col9a1 +/+ ) littermates were examined by light microscopy. Immunohistochemical staining was performed to expression matrix metalloproteinase 3 (MMP‐3) MMP‐13, degraded II collagen, discoidin domain receptor 2 (DDR‐2) knee joints. Cartilage mechanics also evaluated...
To investigate whether heterozygosity for a loss-of-function mutation in the gene encoding alpha1 chain of type XI collagen (Col11a1) mice (chondrodysplasia, cho) causes osteoarthritis (OA), and to understand biochemical biomechanical effects this on articular cartilage knee temporomandibular (TM) joints.Articular from TM joints heterozygous cho (cho/+) their wild-type littermates (+/+) was examined. The morphologic properties were evaluated, fibrils examined by transmission electron...
Abstract Objective To investigate the role of collagen receptor discoidin domain 2 (DDR‐2) in pathogenesis osteoarthritis (OA). Methods Histologic and immunohistochemical analyses were performed to characterize femoral head cartilage from 7 patients with OA 4 fracture, as well articular knee joints mice surgically induced OA. Gene constructs encoding human Raf kinase inhibitor protein (RKIP), DDR‐2 lacking (DS) (ΔDS‐DDR‐2) or tyrosine (PTK) core (ΔPTK‐DDR‐2), containing a substitution for...
To investigate whether the reduction of discoidin domain receptor 2 (DDR-2), a cell membrane tyrosine kinase for native type II collagen, attenuates progression articular cartilage degeneration in mouse models osteoarthritis (OA).Double-heterozygous (type XI collagen-deficient [Col11a1(+/-)] and Ddr2-deficient [Ddr2(+/-)]) mutant mice were generated. Knee joints Ddr2(+/-) subjected to microsurgical destabilization medial meniscus. Conditions from knee double-heterozygous surgically treated...
This study is to investigate the possible role of high temperature requirement A 1 (HtrA1) in articular cartilage degeneration. Paraffin sections were prepared from knee and temporomandibular (TM) joints four mouse OA models; two models had a genetic mutation (type IX collagen-deficient type XI collagen-haploinsufficient) surgically induced (destabilization medial meniscus joint discectomy TM joint). The HtrA1 protein expression profiles examined by immunohistostaining. level mRNA taken one...
Joints that have degenerated as a result of aging or injury contain dead chondrocytes and damaged cartilage. Some studies suggested chondrocyte death precedes cartilage damage, but how the loss affects integrity is not clear. In this study, we examined whether undermines in using rapid 3D confocal imaging technique coupled with standard histology. We induced autonomous expression diphtheria toxin to kill articular surface mice determined did lead damage. Moreover, damage after surgical...
Collagens are major components of the ECM in various organs, including lungs. Ectopic expression collagens can regulate tumor progression and disease outcome through remodeling extracellular matrix (ECM). However, it remains largely unexplored whether involved lung adenocarcinoma (LUAD). Analysis three LUAD transcriptional profiles showed that COL10A1 mRNA was up-regulated associated with poor prognosis. Gain- loss-of-function studies were performed to observe promotes cell proliferation...
The goal of this study is to determine whether transforming growth factor beta 1 (Tgf-β1) induces the high temperature requirement A1 (HtrA1) in articular chondrocytes two mouse models osteoarthritis (OA). Early onset cartilage degeneration was characterized by histology. Expression profiles Tgf-β1, p-Smad1, p-Smad2/3 and HtrA1 knee joints were examined immunofluorescene. By vitro ex vivo experiments, human primary cartilages from femoral condyles mice treated with recombinant TGF-β1 an ALK5...
Abstract Current understanding of micropapillary (MP)‐subtype lung adenocarcinoma (LUAD) remains confined to biological activities and genomic landscapes. Unraveling the major regulatory programs underlying MP patterned malignancy offers opportunities identify more feasible therapeutic targets for patients with LUAD. This study shows that subtype LUAD have aberrant activation MYC pathway compared other subtypes. In vitro xenograft mouse model studies reveal pattern in cannot be solely due...
ABSTRACT Aberrantly expressed LINC00942 is participated in the progression of several cancers. However, function esophageal cancer (ESCA) unclear. The objective this study was to explore effect on ESCA and its possible molecular mechanisms. First, differentially lncRNAs were analyzed using GSE192662 microarray. catRAPID omics v2.1 applied predict proteins that might interact with LINC00942. SDS‐PAGE silver staining assay, RNA pull down, RIP assay utilized validate interacting Then, seq...
Abstract Objective To investigate the relationship between increased discoidin domain receptor 2 (DDR‐2) expression and cartilage damage in osteoarthritis (OA). Methods Full‐thickness tissue samples from 16 human knee joints were obtained grade of was evaluated according to Mankin scale. Expression DDR‐2, matrix metalloproteinase 13 (MMP‐13), MMP‐derived type II collagen fragments visualized immunohistochemically. Moreover, upon stimulation with either or gelatin, levels DDR‐2 MMP‐13...
Objective The objective was to characterize the contralateral non‐surgical temporomandibular joint ( TMJ ) in mice that had an opposing osteoarthrosis( OA )‐like induced by unilateral partial discectomy. Methods s on one side were subjected Both surgical and collected at 4, 8, 12 16 weeks post‐surgery for histological examination. morphology of articular cartilage condyle evaluated using a scoring system. Results A progression degeneration seen following discectomy, including increased...
Abstract Osteoarthritis (OA) pathogenesis is mediated largely through the actions of proteolytic enzymes such as matrix metalloproteinase (MMP) 13. The transcriptional regulator CITED2, which suppresses expression MMP13 in chondrocytes, induced by interleukin (IL)‐4 T cells and macrophages, moderate mechanical loading chondrocytes. We tested hypothesis that CITED2 mediates cross‐talk between IL‐4 signaling loading‐induced pathways result chondroprotection, at least part, downregulating...
Activated mast cells are involved in the pathogenesis of allergic rhinitis (AR). As a member matrix metalloproteinase (MMP) family, MMP9 has been previously demonstrated act pro‑inflammatory manner. Mast regulate activity MMP9, and themselves have reported to produce MMP9. However, best our knowledge, involvement cell activation remains be elucidated. The present study an upregulation protein mRNA expression levels activated by phorbol ester ionomycin. Phosphorylated ERK AKT also markedly...