A5001185170- Chemical Synthesis and Analysis
- Chemistry and Chemical Engineering
- Microwave-Assisted Synthesis and Applications
- Click Chemistry and Applications
- Chemical Synthesis and Reactions
- Multicomponent Synthesis of Heterocycles
- Protein Hydrolysis and Bioactive Peptides
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Machine Learning in Bioinformatics
- Carbohydrate Chemistry and Synthesis
- Gyrotron and Vacuum Electronics Research
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Biochemical and Structural Characterization
Mi Corporation (United States)
2007-2020
St. Matthew's University
2020
Abstract Microwave energy represents an efficient manner to accelerate both the deprotection and coupling reactions in 9‐fluorenylmethyloxycarbonyl (Fmoc) solid phase peptide synthesis (SPPS). Typical SPPS side including racemization aspartimide formation can occur with microwave but easily be controlled by routine use of optimized methods. Cysteine, histidine, aspartic acid were susceptible during a model 20mer containing all 20 natural amino acids. Lowering temperature from 80 °C 50...
A series of improvements to the standard solid phase peptide synthesis (SPPS) process allowing for significant gains in product purity along with only a 4 min cycle time and 90% reduction total waste produced is reported. For example, syntheses well-known 65–74acyl carrier protein (ACP) 1–42β-amyloid peptides were accomplished 93 72% (UPLC–MS) 44 229 min, respectively.
The increasing demand for synthetic peptides in various research fields raises a challenge, namely, the development of greener methods their preparation. Here we report on an ecofriendly solid-phase peptide synthesis (SPPS) methodology. Substitution hazardous solvent DMF by biomass-derived organic γ-valerolactone (GVL), together with application microwave-assisted automated SPPS, allowed wide range lengths and high purity using polystyrene- polyethylene-glycol-based resins. yields achieved...
We present a process for solid phase peptide synthesis (SPPS) that completely eliminates all solvent intensive washing steps during each amino acid addition cycle. A key breakthrough is the removal of volatile Fmoc deprotection base through bulk evaporation at elevated temperature while preventing condensation on vessel surfaces with directed headspace gas flushing. This was demonstrated both research and production scales without any impact product quality when applied to variety...
In solid-phase peptide synthesis, dichloromethane is the predominant solvent used to incorporate first amino acid on a 2-chlorotrityl chloride resin (via nucleophilic substitution) and Wang activation with carbodiimide in presence of 4-dimethylaminopyridine). However, legal authorities have restricted use this solvent, as it considered hazardous potential occupational carcinogen. Therefore, there need for an alternative that easy handle poses less risk environment more importantly human...
The synthesis of peptides for both research and API production is conveniently carried out using the solid-phase strategy. However, main drawback this approach large amounts hazardous solvents required. In regard, several pharmaceutical industries participating in ACS Green Chemistry Institute Pharmaceutical Roundtable have shown interest identifying green peptide synthesis. a previous study, our group proposed γ-valerolactone (GVL) as replacement DMF. during Fmoc removal less hindered Gly...
Anisole and 1,3-dimethoybenzene are excellent alternatives to DCM for cleaving protected peptides.
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