Mili Ramani
A5071952072- Pancreatic and Hepatic Oncology Research
- Caveolin-1 and cellular processes
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- AI in cancer detection
- Blood Coagulation and Thrombosis Mechanisms
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Hemophilia Treatment and Research
- Immune Response and Inflammation
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Blood properties and coagulation
- Macrophage Migration Inhibitory Factor
- Phagocytosis and Immune Regulation
- Protease and Inhibitor Mechanisms
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
Johns Hopkins University
2024-2025
The University of Texas at Austin
2025
Johns Hopkins Medicine
2024-2025
Bloomberg (United States)
2024
University of Baltimore
2024
Sidney Kimmel Comprehensive Cancer Center
2024
Inserm
1993-1995
Abstract Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterized by immunosuppressive tumor microenvironment enriched with cancer-associated fibroblasts (CAF). This study used a convergence approach to identify cell and CAF interactions through the integration of single-cell data from human tumors organoid coculture experiments. Analysis comprehensive atlas PDAC RNA sequencing indicated that density associated increased inflammation epithelial–mesenchymal transition...
Pro1 is a critical catalytic residue in the characterized activities of tautomerase superfamily (TSF) members. Only handful members (∼346) lack sequence similarity network (SSN) that consists over 11,000 Most (294 members) are malonate semialdehyde decarboxylase (MSAD)-like subgroup, but ones thus far have little or no MSAD activity. Moreover, there to activity with other TSF substrates. Five non-Pro1 were selected randomly for kinetic [using phenylenolpyruvate (PP) and 2-hydroxymuconate...
Abstract Pancreatic ductal adenocarcinoma (PDAC) carries an extremely poor prognosis, in part resulting from cellular heterogeneity that supports overall tumorigenicity. Cancer associated fibroblasts (CAF) are key determinants of PDAC biology and response to systemic therapy. While CAF subtypes have been defined, the effects patient-specific plasticity on tumor cell behavior remain unclear. Here, multi-omics was used characterize microenvironment (TME) tumors patients undergoing...
In Gram‐negative septic shock, human monocytes synthesize and express on their cytoplasmic membrane tissue factor (TF), a potent activator of the coagulation cascades. The role TF in triggering disseminated intravascular (DIC) these patients appears to be clear. We report suppressive effect interleukin‐10 (IL‐10) endotoxin‐induced activity antigen levels, expression mRNA levels monocytes. These results emphasize potential therapeutic value this cytokine condition still associated with high...
Tissue factor (TF) is a transmembrane receptor which, in association with factors VII and VIIa, activates IX X, thereby activating the coagulation protease cascades. In response to bacterial lipopolysaccharide (LPS) monocytes transcribe, synthesize express TF on their surface. We investigated whether LPS-induced human mediated by protein kinase C (PKC) activation. The PKC agonists phorbol 12-myristate 13-acetate (PMA) 12, 13 dibutyrate (PdBu) were both potent inducers of monocytes, whereas 4...
Fibrin deposition is an integral feature of the inflammatory response. In response to C‐reactive protein (CRP), acute‐phase reactant, blood monocytes synthesize and express tissue factor (TF), main initiator coagulation. We report inhibitory effect interleukin 10 (IL‐10) that pentoxifylline, a methyl xanthine derivative, on monocyte expression TF activity, mRNA in CRP. These agents may be use diseases where TF‐induced prothrombotic state detrimental.
Tissue factor (TF) is a transmembrane glycoprotein which assembles with VIIa on cell surfaces to form proteolytically active cofactor-enzyme complex; the TF/VIIa complex initiates coagulation protease cascade. In response bacterial lipopolysaccharide (LPS) and phorbol-12 myristate 13-acetate (PMA), monocytes synthesize express TF their surface. However, mechanisms by LPS PMA activate synthesis human blood are not fully understood. As it has been established that protein tyrosine kinase (PTK)...
Abstract Introduction: Pancreatic ductal adenocarcinoma (PDAC) is a heterogeneous tumor comprised of epithelial tumor, endothelial, immune, and importantly, cancer associated fibroblasts (CAFs) cells. CAFs drive complex microenvironment (TME) through mechanisms intratumoral interactions that are incompletely understood. It’s crucial to account for the role in modern ex-vivo systems as regulators both promoting restraining growth, providing growth factor support, reprogramming immune cells,...
Abstract Introduction: A complex immunosuppressive tumor microenvironment (TME) is a distinctive feature of pancreatic ductal adenocarcinoma (PDAC). The TME consists an admixture cell types including epithelial, endothelial, immune cells both myeloid and lymphoid origin, cancer-associated fibroblasts (CAFs). traditionally defined subtypes PDAC include classical, associated with the retention epithelial markers better prognosis, basal, which characterized by quasi-mesenchymal phenotype poorer...
<p>Pattern 1 Pathways</p>
<div>Abstract<p>Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterized by immunosuppressive tumor microenvironment enriched with cancer-associated fibroblasts (CAF). This study used a convergence approach to identify cell and CAF interactions through the integration of single-cell data from human tumors organoid coculture experiments. Analysis comprehensive atlas PDAC RNA sequencing indicated that density associated increased inflammation...
<p>Pancreas Gene Markers</p>
<p>Pattern_7_ordered_genes</p>
<p>Supplementary methods and figures</p>
<p>Pattern 1 Pathways</p>
<div>Abstract<p>Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterized by immunosuppressive tumor microenvironment enriched with cancer-associated fibroblasts (CAF). This study used a convergence approach to identify cell and CAF interactions through the integration of single-cell data from human tumors organoid coculture experiments. Analysis comprehensive atlas PDAC RNA sequencing indicated that density associated increased inflammation...
<p>Supplementary methods and figures</p>
<p>Pattern_7_ordered_genes</p>
<p>Pancreas Gene Markers</p>