A5113325453- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Reproductive tract infections research
- Pneumonia and Respiratory Infections
Sriram Chandra Bhanja Medical College Hospital
2024-2025
Ceres Nanosciences
2020-2024
Abstract Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION platform. Our approach utilizes novel affinity-based particle, circumventing low input sample volumes allowing both rapid manual automated high throughput workflows are compatible with sequencing. This enhances standard RNA extraction protocols,...
Abstract Here we present a rapid and versatile method for capturing concentrating SARS-CoV-2 from contrived transport medium saliva samples using affinity-capture magnetic hydrogel particles. We demonstrate that the concentrates virus 1 mL prior to RNA extraction, substantially improving detection of real-time RT-PCR across range viral titers (100–1,000,000 copies/mL) enabling 2019 nCoV CDC EUA Kit down 100 copies/mL. This is compatible with commercially available nucleic acid extraction...
Abstract Here we present a rapid and versatile method for capturing concentrating SARS-CoV-2 from transport medium saliva using affinity-capture magnetic hydrogel particles. We demonstrate that the concentrates virus prior to RNA extraction, thus significantly improving detection of real-time RT-PCR assay across range viral titers, 100 1,000,000 copies/mL; in particular, low load samples is enhanced when coupled with IDT 2019-nCoV CDC EUA Kit. This compatible commercially available nucleic...
Abstract Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION platform. Our approach utilizes novel affinity-based particle, circumventing low input sample volumes allowing both rapid manual automated high throughput workflows are compatible with nanopore sequencing. This enhances standard RNA extraction...
Abstract Wastewater testing has emerged as an effective tool for monitoring levels of SARS-CoV-2 infection in sewered communities. As July 2024, PCR-based methods continue to be the most widely used wastewater surveillance (1–3). Data from is usually available public health authorities near real time, typically within 5 7 days after waste enters sewer (4,5). Unfortunately, while these can accurately detect and quantify SARS-CoV-2, they are not differentiate between multitude variants,...
: Respiratory Syncytial Virus (RSV) is one of the most common causes acute lower respiratory infection (ALRI) in paediatric age group. Various demographic and risk factors are associated with severity disease. This study was conducted to detect RSV from ALRI cases using two different methods correlate findings various factors. The carried out over a period 2 years on 242 Paediatrics Department SCB Medical College Hospital, Cuttack. Nasopharyngeal aspirates were collected standard procedures...