A5045272584- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Organoselenium and organotellurium chemistry
- Sulfur-Based Synthesis Techniques
- RNA Research and Splicing
- Click Chemistry and Applications
University of Toronto
2023-2024
University of Alberta
2023
Tellurophene-bearing small molecules have emerged as valuable tools for localizing cellular activities
CRISPR-Cas9 is currently the most versatile technique to perform gene editing in living organisms. In this approach, Cas9 endonuclease guided toward its DNA target sequence by guide RNA (gRNA). Chemical synthesis of a functional single gRNA (sgRNA) nontrivial because length strand. Recently we demonstrated that sgRNA can be stitched together from three smaller fragments through copper-catalyzed azide-alkyne cycloaddition, making process highly modular. Here further advance approach...
CRISPR-Cas9 is currently the most versatile technique to perform gene editing in living organisms. In this approach, Cas9 endonuclease guided towards its DNA target sequence by guide RNA (gRNA). Chemical synthesis of a functional 97-nucleotide single gRNA (sgRNA) non-trivial because length strand. Recently, we demonstrated that sgRNA can be stitched together from three smaller fragments through copper-catalyzed azide-alkyne cycloaddition, making process highly modular. Here, further advance...