Rapid, precise, and reproducible deposition of a broad variety functional materials, including analytical assay reagents biomolecules, has made inkjet printing an effective tool for the fabrication microanalytical devices. A ubiquitous office device as simple standard desktop printer with its multiple ink cartridges can be used this purpose. This Review discusses combination technology paper substrate microfluidic paper-based devices (μPADs), which have developed into fast-growing new field...

"Distance-based" detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize cost-effective and calibration-free distance-based assay of lactoferrin human tear fluid μPAD not relying antibodies or enzymes, we investigated the fluidic mobilities target protein Tb(3+) cations used as fluorescent reagent surface-modified cellulosic filter papers....

An inkjet-printed microfluidic paper-based analytical device (μPAD) for the detection of lactoferrin has been developed. The analyte concentration dependent fluorescence emission, caused by sensitization pre-deposited terbium (Tb3+) upon complexation with on paper device, is captured using a digital camera. dynamic response range (0.5–3 mg mL−1) and limit (0.30 μPAD are suitable analysis normal human tears eye disorders. Finally, concentrations in tear samples were analyzed μPADs assay...

This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for analysis sub-microliter sample volumes. The wax-printing approach widely used patterning paper substrates has been adapted to obtain high-resolution structures patterned in filter paper. achieved by replacing hot plate heating method conventionally melt printed wax features into simple lamination. technique, combination with consideration device geometry and influence cellulose fiber...

This paper describes a paper-based analytical device allowing the direct semiquantitative interpretation of result chemical assay in form "text". The combined use classical colorimetric indicator system and an additional inert colorant enables versatile text-displaying detection mechanism on device. For proof-of-concept, urinary protein has been selected as model target. whole developed based printing techniques including wax printing, inkjet 3D printing. results user tests performed with...

An improved paper-based analytical device (PAD) using color screening to enhance performance is described.

In this paper, we propose a human activity recognition method from first-person videos, which provides supplementary to improve the accuracy. Conventional methods detect objects and derive user's behavior based on their taxonomy. One of recent works has achieved accuracy improvement by determining key hand manipulation. However, such manipulation-based approach restriction applicable scenes object types because hands don't always present significant information. contrast, our proposed...

On the example of a colorimetric sodium assay, this work demonstrates implementation classical cation-exchange optode relying on an ionophore-doped plasticized PVC membrane into paper-based analytical device (PAD). An ion-selective (ISO) system has been arranged vertically-assembled PAD (vPAD) integrating pH-buffering function. Capillary force-driven sample liquid transportation through paper matrix enabled pH-adjustment prior to optical detection analyte cation. Functionalized layers with...

The transport efficiency during capillary flow-driven sample on microfluidic paper-based analytical devices (μPADs) made from filter paper has been investigated for a selection of model analytes (Ni2+, Zn2+, Cu2+, PO43-, bovine serum albumin, sulforhodamine B, amaranth) representing metal cations, complex anions, proteins and anionic molecules. For the first time, target compounds rather than liquid, quantitatively evaluated by means colorimetry absorption spectrometry-based methods....

Abstract Background The Direct Fluorescent Antibody Test (DFAT), the standard rabies confirmatory test, is less sensitive when used with decomposed brain samples, a frequent issue in rabies-endemic regions. This study evaluates diagnostic accuracy of ADTEC lateral flow device (LFD) for post-mortem diagnosis using samples. Methods We 34 animal heads submitted cold chain to an laboratory located central Philippines including 26 DFAT-positive and eight DFAT-negative After defrosting heads,...

This work describes the generation of plasticized poly(vinyl chloride)-based ion-selective optode (ISO) nanoparticles by a piezoelectric inkjet dispensing device. The monodisperse inkjet-generated particles (polydispersity index: 0.177) with an average hydrodynamic diameter 202 nm are suitable for highly reproducible deposition onto cellulosic filter paper substrates, resulting in paper-based analytical devices (PADs) colorimetric cation detection, as demonstrated on example...

Insufficient sensitivity is a general issue of colorimetric paper-based analytical devices (PADs) for trace analyte detection, such as metal ions, in environmental water. This paper demonstrates the detection zinc ions (Zn2+) on device with an integrated concentration system. Concentration Zn2+ from enlarged sample volume (1 mL) has been achieved aid indicator (Zincon) electrostatically immobilized onto filter substrate combination highly water-absorbent materials. Analyte well pretreatment,...

In this study, we develop a model that enables mobilities to have more friendly interactions with users. Specifically, focus on the referring navigable regions task in which grounds of road using mobility's camera image and natural language navigation instructions. This is challenging because requirement vision-and-language comprehension situations involve rapidly changing environments other mobilities. The performance existing methods insufficient, partly they do not consider features...

Abstract Die Möglichkeit zur schnellen, präzisen und reproduzierbaren Auftragung einer Vielzahl von funktionellen Materialien, einschließlich analytischer Testreagentien Biomoleküle, hat den Tintenstrahldruck zu einem effektiven Verfahren für die Fabrikation mikroanalytischen Systemen gemacht. Ein allgegenwärtiges Bürogerät, so einfach wie ein Desktop‐Tintenstrahldrucker mit mehreren Tintenpatronen, kann diesem Zweck verwendet werden. Dieser Aufsatz diskutiert Kombination Papier als...

Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited sensitivity, which in classical immunoassay formats overcome enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, hampered the complexity procedure requiring enzyme substrate addition after sample addition. In this work, a simple method for automation without user...