A5060158609- Cancer, Hypoxia, and Metabolism
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Advanced Fluorescence Microscopy Techniques
- Thermal Regulation in Medicine
- ATP Synthase and ATPases Research
- Histone Deacetylase Inhibitors Research
- bioluminescence and chemiluminescence research
- Pediatric Urology and Nephrology Studies
- Multiple Myeloma Research and Treatments
- Urinary Tract Infections Management
- Lung Cancer Research Studies
- Epigenetics and DNA Methylation
- Cardiac Ischemia and Reperfusion
- Glioma Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- Signaling Pathways in Disease
- Cardiovascular Function and Risk Factors
- Cancer Treatment and Pharmacology
- Bladder and Urothelial Cancer Treatments
- Organ Transplantation Techniques and Outcomes
University of Tartu
2021-2024
Since 1991, the NAD(P)H-aided conversion of resazurin to fluorescent resorufin has been widely used measure viability based on metabolic activity in mammalian cell culture and primary cells. However, different research groups have divergent assay protocols, scarcely reporting systematic optimization assay. Here, we perform extensive studies fine-tune experimental protocols utilizing resazurin-based sensing. Specifically, focus (A) dynamic range individual lines for correct measurement...
The choice of targeted therapies for treatment glioblastoma patients is currently limited, and most die from the disease recurrence. Thus, systematic studies in simplified model systems are required to pinpoint targets further exploration clinical settings. Here, we report screening 5 compounds targeting epigenetic writers or erasers 6 cell cycle-regulating protein kinases against 3 lines following incubation under normoxic hypoxic conditions. viability/proliferation assay indicated that...
Ischemia reperfusion injury is common in transplantation. Previous studies have shown that cooling can protect against hypoxic injury. To date, the protective effects of hypothermia been largely associated with metabolic suppression. Since kidney transplantation one most organ transplant surgeries, we used human-derived renal proximal tubular cells (HKC8 cell line) as a model normal cells. We performed temperature titration curve from 37 °C to 22 and evaluated cellular respiration molecular...
Abstract Multiple myeloma is an incurable cancer of plasma cells that depends on the bone marrow for its survival. Despite prevalence, molecular mechanisms underlying this malignancy remain poorly understood. In study, we aim to bridge knowledge gap by elucidating metabolic interplay between and mesenchymal stem (BMMSCs). BMMSCs are crucial in supporting cell metabolism, contributing their proliferation, survival, resistance chemotherapy. Through a combination mathematical modelling...
Heritable renal cancer syndromes (RCS) are associated with numerous chromosomal alterations including inactivating mutations in von Hippel-Lindau (VHL) gene. Here we identify a novel aspect of the phenotype VHL-deficient human cells. We call it reductive stress as is characterised by increased NADH/NAD+ ratio that impaired cellular respiration, CAC activity, upregulation carboxylation glutamine and accumulation lipid droplets Reductive was mitigated glucose depletion supplementation pyruvate...
The demands of cancer cell proliferation alongside an inadequate angiogenic response leads to insufficient oxygen availability in the tumour microenvironment. Within mitochondria, is major electron acceptor for NADH, with result that reducing activity tricarboxylic acid (TCA) cycle and mitochondrial respiration are functionally linked. As oxidising TCA required efficient synthesis anabolic precursors, tumoral hypoxia could lead a cessation without another means correcting redox imbalance. We...
Abstract The choice of targeted therapies for treatment glioblastoma patients is currently limited, and most die from the disease recurrence. Thus, systematic studies in simplified model systems are required to pinpoint targets further exploration clinical settings. Here, we report screening 5 compounds targeting epigenetic writers or erasers 6 cell cycle-regulating protein kinases against 3 lines following incubation under normoxic hypoxic conditions. viability assay indicated that PRMT5...