A5067779758- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Diet and metabolism studies
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- BRCA gene mutations in cancer
- Phagocytosis and Immune Regulation
- Fatty Acid Research and Health
- Menopause: Health Impacts and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism, Diabetes, and Cancer
- Estrogen and related hormone effects
- Biotin and Related Studies
- Alzheimer's disease research and treatments
- Insect Resistance and Genetics
- Mechanisms of cancer metastasis
- Lipid metabolism and disorders
- Studies on Chitinases and Chitosanases
- Cardiovascular Disease and Adiposity
- Effects and risks of endocrine disrupting chemicals
- Biochemical effects in animals
- Phytoestrogen effects and research
- Nutrition, Genetics, and Disease
Karolinska Institutet
2023
University of Maryland, College Park
2010-2019
North Dakota State University
2013
University of Missouri
2010
Queen's Medical Centre
1996
Measurement of mitochondrial function in skeletal muscle is a vital tool for understanding regulation cellular bioenergetics. Currently, number different experimental approaches are employed to quantify function, with each involving either mechanically or chemically induced disruption membranes. Here, we describe novel approach that allows the quantification substrate-induced mitochondria-driven oxygen consumption intact single fibers isolated from adult mice. Specifically, flexor digitorum...
Disruptions of ovarian function in women are associated with increased risk metabolic disease due to dysregulation peripheral glucose homeostasis skeletal muscle. Our previous evidence suggests that alterations muscle lipid metabolism coupled altered mitochondrial may also develop. The objective this study was use an integrative approach identify potential areas dysfunction develop from ovariectomized (OVX) female mice compared age-matched ovary-intact adult (sham). OVX exhibited significant...
Age is considered a primary risk factor for neurodegenerative diseases including Alzheimer's disease (AD). It also now well understood that mitochondrial function declines with age. Mitochondrial deficits have been previously assessed in brain from both human autopsy tissue and disease-relevant transgenic mice. Recently it has recognized abnormalities of muscle may be an intrinsic aspect AD might contribute to the pathophysiology. However, yet clearly tissues outside central nervous system...
Breast cancer type 1 (BRCA1) susceptibility protein is expressed across multiple tissues including skeletal muscle. The overall objective of this investigation was to define a functional role for BRCA1 in muscle using translational approach. For the first time both mice and humans, we identified presence isoforms In response an acute bout exercise, found increases interaction between native forms phosphorylated form acetyl-CoA carboxylase. Decreasing <i>BRCA1</i> content shRNA approach...
Pancreatic carcinoma lacks effective therapeutic strategies resulting in poor prognosis. Transcriptional dysregulation due to alterations KRAS and MYC affects initiation, development, survival of this tumor type. Using patient-derived xenografts KRAS- MYC-driven pancreatic carcinoma, we show that coinhibition topoisomerase 1 (TOP1) bromodomain-containing protein 4 (BRD4) synergistically induces regression by targeting promoter pause release. Comparing the nascent transcriptome with...
Estrogen receptor-α (ERα) is a nuclear receptor family member thought to substantially contribute the metabolic regulation of skeletal muscle. However, previous mouse models utilized assess necessity ERα signaling in muscle were confounded by altered developmental programming and/or influenced secondary effects, making it difficult assign causal role for ERα. The objective this study was determine regulating metabolism absence confounding factors development. A novel model developed allowing...
Decreases in female sex steroids enhance the accumulation of visceral fat mass, leading to a predisposition developing metabolic diseases. The purpose this study was determine whether loss ovarian function alters amount and (or) fatty acid (FA) composition triacylglycerol (TAG) levels liver ovary-intact (SHAM) or ovariectomized (OVX) mice. We also sought voluntary wheel running could attenuate associated changes liver. Twenty-two C57/BL6 mice were divided into 2 groups (SHAM, OVX) then...
The breast cancer type 1 susceptibility protein (Brca1) is a regulator of DNA repair in mammary gland cells; however, recent cell culture evidence suggests that Brca1 influences other processes, including those nonmammary cells. In this study, we sought to determine whether necessary for metabolic regulation skeletal muscle using novel vivo mouse model. We developed an inducible muscle-specific Brca1-knockout (BRCA1KOsmi) model test expression maintenance function when exposed high-fat diet...
Abstract Matrix metalloproteinases (MMPs) are a class of zinc‐dependent endopeptidases that highly conserved across numerous taxa, from bacteria to humans. Recently, MMPs have been identified in several insect species and hypothesized function immunity development. In this study, we identify putative MMP correlate its proteolytic activity gene protein expression the tracheae with developmental stage. Ms‐MMP increases 10‐fold during molting, which is accompanied by an increase both...
Impaired ovarian function alters lipid metabolism, ultimately resulting in increased visceral fat mass. Currently, we have a poor understanding of alterations signaling events regulating lipolysis after declines. The purpose this study was to determine if cellular mechanisms are altered mice ovariectomy (OVX) and OVX exhibit impaired lipolytic when stimulated by acute exercise. SHAM were divided into two groups: control (SHAM cont; cont) or treadmill exercise ex; ex). omental/mesenteric...
Key points Breast cancer 1 early onset gene codes for the DNA repair enzyme, breast type susceptibility protein (BRCA1). The is prone to mutations that cause a loss of function. BRCA1/Brca1 has recently been found regulate several cellular pathways beyond and expressed in skeletal muscle. Skeletal muscle specific knockout Brca1 mice caused quality, identifiable by reductions force production mitochondrial respiratory capacity. Loss quality was associated with shift phenotype an accumulation...
Large amounts of visceral fat mass are correlated with the risk developing numerous chronic diseases. We have previously shown that loss ovarian function results in substantial increases mass. The purpose this study was to determine if altered amount and/or composition triacylglycerol (TAG) liver from SHAM, ovariectomized (OVX), or estradiol supplemented OVX mice. In addition, we sought increasing physical activity levels mice an effective intervention for attenuating any lipid based changes...
Breast cancer early onset type 1 (BRCA1) is an estrogen sensitive gene known to play a critical role in the prevention breast tumorigenesis and also thought contribute DNA damage repair. Recently tissue, BRCA1 was identified as binding partner regulator of acetyl CoA carboxylase (ACC) activity suggesting may act lipid metabolism. Currently, has yet be characterized skeletal muscle. The purpose this study determine if expressed muscle it could immunoprecipated with phosphorylated form ACC...