A5004977926- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Nuclear Structure and Function
- Erythrocyte Function and Pathophysiology
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Heat shock proteins research
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
Xiamen University
2022-2024
Focal adhesions (FAs) are transmembrane protein assemblies mediating cell–matrix connection. Although liquid–liquid phase separation (LLPS) has been tied to the organization and dynamics of FAs, underlying mechanisms remain unclear. Here, we experimentally tune LLPS PXN/Paxillin, an essential scaffold by utilizing a light-inducible Cry2 system in different cell types. In addition nucleating FA components, light-triggered PXN potently activates integrin signaling subsequently accelerates...
The progression of breast cancer is often accompanied by changes in extracellular matrix stiffness and cell adhesion ability, which are closely related to cellular mechanotransduction. However, the underlying regulatory mechanisms remain mysterious. Our study reveals that macroautophagy/autophagy-inducing kinases, ULK1 ULK2, inhibit assembly focal adhesions F-actin phosphorylating protein PXN, prevent migration an autophagy-independent fashion. Interestingly, ULK1/ULK2-mediated serine...
Abstract Focal adhesions (FAs) are transmembrane protein assemblies mediating cell-matrix connection. Tools to manipulate the compositionally intricate and dynamic FAs currently limited, rendering many fundamental hypotheses untestable. Although liquid-liquid phase separation (LLPS) has been tied organization dynamics of FAs, underlying mechanisms remain unclear. Here, we experimentally tune LLPS PXN/Paxillin, an essential scaffold by utilizing light-inducible Cry2 system. In addition...
Abstract The remodeling and stiffening of the extracellular matrix (ECM) associated with breast cancers is a well-recognized modulator disease progression. However, how changes in mechanical properties ECM are converted into biochemical signals that direct tumor cell migration metastasis remains poorly characterized. Here, we describe new role for autophagy-inducing serine/threonine kinases ULK1 ULK2 mechanotransduction. We demonstrate ULK1/2 activity inhibits assembly actin stress fibers...