A5076030250- Enzyme Structure and Function
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Protein Structure and Dynamics
- Microbial bioremediation and biosurfactants
- Pharmacogenetics and Drug Metabolism
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Biotin and Related Studies
- Glycosylation and Glycoproteins Research
- Metal-Catalyzed Oxygenation Mechanisms
- Amino Acid Enzymes and Metabolism
- Reproductive System and Pregnancy
- 14-3-3 protein interactions
- Steroid Chemistry and Biochemistry
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Immunotherapy and Immune Responses
- Urologic and reproductive health conditions
- PARP inhibition in cancer therapy
- Biochemical and Molecular Research
- Mesenchymal stem cell research
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Insect-Plant Interactions and Control
Hubei University
2021-2025
First Affiliated Hospital of Anhui Medical University
2021-2024
Anhui Medical University
2021-2024
Shanghai Institutes for Biological Sciences
2006-2019
St. Jude Children's Research Hospital
2017
Wuhan Institute of Physics and Mathematics
2011-2014
Chinese Academy of Sciences
2006-2014
Shanghai Jiao Tong University
2006
The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation PET industry. In present study, we demonstrate that post-translational glycosylation Pichia pastoris makes it a remarkable host heterologous expression PETase from Ideonella sakaiensis 201-F6 (IsPETase). Taking advantage abundant N- and O-linked sites IsPETase efficient modification endoplasmic reticulum, heavily glycosylated during secretory with P. pastoris,...
Inorganic polyphosphate (polyP) is an ancient energy metabolite and phosphate store that occurs ubiquitously in all organisms. The vacuolar transporter chaperone (VTC) complex integrates cytosolic polyP synthesis from ATP membrane translocation into the lumen. In yeast other eukaryotes, regulated by inositol pyrophosphate (PP-InsP) nutrient messengers, directly sensed VTC complex. Here, we report cryo-electron microscopy structure of signal-activated at 3.0 Å resolution. Baker's subunits...
Many steroids are important pharmaceutically active compounds, while cytochrome P450 monooxygenases (CYPs) attractive enzymes for applications in steroidal drug synthesis. However, the catalytic efficiency of existing P450s is not routinely high enough, as well molecular basis selectivity control unclear, which severely restrict their real applications. Here, a 16β steroid-hydroxylase CYP109B4 from Bacillus sonorensis identified with excellent and activity. The crystallization structural...
Focal adhesions (FAs) are transmembrane protein assemblies mediating cell–matrix connection. Although liquid–liquid phase separation (LLPS) has been tied to the organization and dynamics of FAs, underlying mechanisms remain unclear. Here, we experimentally tune LLPS PXN/Paxillin, an essential scaffold by utilizing a light-inducible Cry2 system in different cell types. In addition nucleating FA components, light-triggered PXN potently activates integrin signaling subsequently accelerates...
Another kind of dynamics: Ubiquitin noncovalently dimerizes with a dissociation constant approximately 5 mM. The two subunits adopt an array relative orientations, utilizing interface also for binding to other proteins (see picture). Quaternary fluctuation among members the dimer ensemble constitutes different dynamics that complements tertiary each ubiquitin subunit. is small signaling protein in cells and highly conserved throughout eukaryotes. interacts myriad partner contain one or more...
Intracellular nicotinamide phosphoribosyltransferase (iNAMPT) in neuron has been known as a protective factor against cerebral ischemia through its enzymatic activity, but the role of central extracellular NAMPT (eNAMPT) is not clear. Here we show that eNAMPT protein level was elevated ischemic rat brain after middle-cerebral-artery occlusion (MCAO) and reperfusion, which can be traced to at least part from blood circulation. Administration recombinant exacerbated MCAO-induced neuronal...
Tumor suppressor p53 prevents tumorigenesis by promoting cell cycle arrest and apoptosis through transcriptional regulation. Dysfunction of occurs frequently in human cancers. Thus, becomes one the most promising targets for anticancer treatment. A bacterial effector protein azurin triggers tumor suppression stabilizing elevating its basal level. However, structural mechanistic basis azurin-mediated remains elusive. Here we report atomic details stabilization combining X-ray crystallography...
Cytochrome P450 enzyme CYP109B1 is a versatile biocatalyst exhibiting hydroxylation activities toward various substrates. However, the regio- and stereoselective steroid by far less explored. In this study, oxidizing activity of reconstituted coupling redox pairs from different sources, or fusing it to reductase domain two self-sufficient enzymes P450RhF P450BM3 generate fused enzyme. The recombinant Escherichia coli expressing necessary proteins are individually constructed compared in...
Abstract Focal adhesions (FAs) are transmembrane protein assemblies mediating cell-matrix connection. Tools to manipulate the compositionally intricate and dynamic FAs currently limited, rendering many fundamental hypotheses untestable. Although liquid-liquid phase separation (LLPS) has been tied organization dynamics of FAs, underlying mechanisms remain unclear. Here, we experimentally tune LLPS PXN/Paxillin, an essential scaffold by utilizing light-inducible Cry2 system. In addition...
Nature has evolved many supramolecular proteins assembled in certain, sometimes even seemingly oversophisticated, morphological manners. The rationale behind such evolutionary efforts is often poorly understood. Here, we provide atomic-resolution insights into how the dynamic building of a structurally complex enzyme with higher order symmetry offers amenability to intricate regulation. We have established functional coupling between enzymatic activity and protein states glutamine synthetase...
The binding affinities between E-group colicin (CE) and cognate immunity proteins (Im) are among the strongest interactions in nature. Aiming at mining protein pairs with ultrahigh affinity for artificial multienzyme complexes, DNase domains of CE2, 8, 9 were engineered to generate CL2, variants without DNA catalytic activity but retaining Im activity, respectively. Further study discovered that all these variants, addition a CE7 variant (CL7) from previous report, their highly thermostable....
Abstract The remodeling and stiffening of the extracellular matrix (ECM) associated with breast cancers is a well-recognized modulator disease progression. However, how changes in mechanical properties ECM are converted into biochemical signals that direct tumor cell migration metastasis remains poorly characterized. Here, we describe new role for autophagy-inducing serine/threonine kinases ULK1 ULK2 mechanotransduction. We demonstrate ULK1/2 activity inhibits assembly actin stress fibers...
Abstract BackgroundEndometrial injury contributes to impaired endometrial receptivity, and is well recognized as a critical factor in implantation failure. Increasing evidence suggests that the therapeutic effects of mesenchymal stem cells (MSCs) mainly depend on their capacity secrete paracrine factors are mediated by MSC-derived exosomes (MSC-Exos). In this study, we aimed explore human umbilical cord cell-derived (hUCMSC-Exos) injured endometrium mouse damage model potential mechanisms...
Abstract BackgroundThere are many studies on the advantages of mesenchymal stem cells (MSCs) that could secret various paracrine factors in repairing endometrial injury. It is necessary to improve stability and effectiveness MSCs. Hepatocyte growth factor (HGF), as one cytokines secreted by MSCs, plays a significant role vascular repair epithelial transformation (MET). can be deduced HGF closely related process endometrium.Therefore, we aim investigate effect mechanism MSCs from umbilical...
Im Gedränge der zellulären Umgebung …︁…︁ geben Proteine Signale weiter, indem sie kurzzeitig, aber spezifisch miteinander wechselwirken. C. Tang, W.-P. Zhang et al. zeigen in Zuschrift auf S. 11685 ff., dass eine Phosphorylierung zwischen zwei Proteinen mit einer Bindungsaffinität von nur 25 mM und Lebensdauer ihres Komplexes im μs- bis ms-Bereich stattfinden kann; die Werte für alle bekannten Protein-Protein-Wechselwirkungen sind um Größenordnung höher. Solche schwachen, flüchtigen...