A5100417821- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Ovarian function and disorders
- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- Sperm and Testicular Function
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Gynecological conditions and treatments
- Cellular Mechanics and Interactions
- Mechanical and Optical Resonators
- Epigenetics and DNA Methylation
- Freezing and Crystallization Processes
- Cystic Fibrosis Research Advances
- Nerve injury and regeneration
- SARS-CoV-2 detection and testing
- Genetic and Kidney Cyst Diseases
- Venomous Animal Envenomation and Studies
- Urological Disorders and Treatments
- Spine and Intervertebral Disc Pathology
- Seed Germination and Physiology
- RNA Interference and Gene Delivery
First Affiliated Hospital of Anhui Medical University
2017-2024
Anhui Medical University
2017-2024
Ministry of Education of the People's Republic of China
2022-2024
Chinese PLA General Hospital
2023
Binzhou Medical University
2022
Anhui Provincial Hospital
2017-2021
Fifth Affiliated Hospital of Zhengzhou University
2021
University of Science and Technology of China
2013-2016
The Ohio State University
2013
The human endometrial stromal cells (hEndoSCs) could maintain homeostasis and play a critical role in repairing injury. Mesenchymal stem (MSCs) significantly increase the proliferation of damaged hEndoSCs protect them from apoptosis. Recent studies indicated that exosomes derived be recruited to tissues for regeneration, which exhibit potential cell therapy as therapeutic vectors. In this study, we isolated umbilical cord mesenchymal cell-derived (hUCMSC-Exos) investigated effects...
Cell transplantation is an emerging and effective therapeutic approach for enhancing uterine adhesions caused by endometrial damage. Currently, human umbilical cord blood mononuclear cells (HUCBMCs) have been extensively tissue organ regeneration. However, their application in repair remains unexplored. Our investigation focuses on the utilization of HUCBMCs treating injury.
Abstract Exosomes derived from mesenchymal stem cells (MSCs) have been confirmed to enhance cell proliferation and improve tissue repair. release their contents into the cytoplasmic solution of recipient mediate expression, which is main pathway through exosomes exert therapeutic effects. The corresponding process exosome internalization mainly occurs in early stage treatment. However, effect remains be further studied. We report that three-dimensional traction force can intuitively reflect...
Abstract Background Ovarian tissue cryopreservation and transplantation are novel therapeutic approaches for fertility preservation. However, follicle loss caused by ischemic hypoxic damage is one of the issues after frozen-thawed ovarian transplantation. Promoting angiogenesis in grafts key to restore cryopreserved function. Mesenchymal stem cells (MSCs) have been reported facilitate risk embolization, immunogenic effect tumorigenesis hinders clinical application MSCs human organ In this...
There are many studies on the advantages of using mesenchymal stem cells (MSCs) that secrete various paracrine factors for repairing endometrial injury. However, stability and effectiveness MSCs require improvement to become a viable therapy. Hepatocyte growth factor (HGF), one cytokines secreted by MSCs, promotes vascular repair epithelial transformation (MET). Therefore, HGF likely process endometrium. We prepared transfected with gene explore its effects mechanism damaged endometrium...
Spinal cord injury is pathologically characterized by the loss of motor function caused neurons apoptosis.Store-operated calcium entry (SOCE) widely known to dictate apoptosis various cell types.To examine SOCE in spinal and explore role apoptosis, patients with (SCI) SCI mouse models were included.Expression components apoptosis-related proteins examined Western blotting.Calcium imaging was used assess activity.As a result, we confirmed enhanced levels ORAI1 STIM1 models.In vitro study,...
To evaluate the embryonic developments and clinical outcomes of different sperm sources with cycles intracytoplasmic injection (ICSI) in vitro maturation (IVM). This retrospective study was approved by hospital ethics committee conducted fertilization (IVF) clinic. From January 2005 to December 2018, 239 infertile couples underwent IVM-ICSI were divided into three groups according sources. Group 1 comprised patients percutaneous epididymal aspiration (PESA; n = 62, 62 cycles), group 2...
Recently, many studies have found that late embryogenesis abundant (LEA) proteins could protect cells from drought, high salinity, and other stress conditions. Because LEA maintain the integrity stability of cell membranes, increase cell's tolerance to dehydration stress, reduce osmotic freezing damage during freezing. Whether cryopreservation improve viability remains be confirmed. In this study, we purified recombinant AavLEA1 proteins, examined their thermal solubility effect on cells,...
Primary ciliary dyskinesia (PCD) is an autosomal recessive hereditary disorder affecting motile cilia structure and function, which leads to respiratory diseases infertility. Here, induced pluripotent stem cell (iPSC) line of PCD was generated from peripheral blood mononuclear cells a female patient carrying biallelic mutations in Cyclin O (CCNO) gene. Reprogramming performed with the non-integrated episomal vectors. The obtained transgene-free iPSCs had normal karyotypes, expressed...
The currently used commercial cooling-rate control device is the liquid nitrogen controlled rate freezer (LNF), which has some shortcomings such as high cost, consumption, and potential operational risks in quality control. Based on thermophysical properties of new materials, we improved, manufactured, optimized a reliable yet simple named "passive cooling rate-controlled (PCD)" with real-time temperature tracing. In this study, using improved PCD cryopreserved human umbilical vein...
Background: Endometrial injury contributes to the reduction of endometrial receptivity and is widely recognized as a critical factor in implantation failure. Increasing evidence suggests that therapeutic effects mesenchymal stem cells (MSCs) mainly depend on their capacity secrete paracrine factors specifically MSC‐derived exosomes (MSC‐Exos), which are regulated by exosomal miRNA. In this study, we investigated human umbilical cord MSC‐Exos (hUCMSC‐Exos) injured endometrium potential...
Oocyte maturation defect-4 (OOMD4) is an autosomal recessive disease characterized by oocyte arrest. On chromosome 15q21, the PATL2 gene mutated, resulting in OOMD4 either a homozygous or compound heterozygous form. Herein, peripheral blood mononuclear cells (PBMCs) were obtained from female patient who was for due to PALT2 mutation. Then we induced pluripotent stem cell (iPSC) using episomal vectors with transcription factors reprogramming. The teratoma assay revealed that iPSC line...
Abstract BackgroundEndometrial injury contributes to impaired endometrial receptivity, and is well recognized as a critical factor in implantation failure. Increasing evidence suggests that the therapeutic effects of mesenchymal stem cells (MSCs) mainly depend on their capacity secrete paracrine factors are mediated by MSC-derived exosomes (MSC-Exos). In this study, we aimed explore human umbilical cord cell-derived (hUCMSC-Exos) injured endometrium mouse damage model potential mechanisms...
Abstract Background: Endometrial injury contributes to impaired endometrial receptivity, and is well recognized as a critical factor in implantation failure. Increasing evidence suggests that the therapeutic effects of mesenchymal stem cells (MSCs) mainly depend on their capacity secrete paracrine factors are mediated by MSC-derived exosomes (MSC-Exos). In this study, we investigated human umbilical cord cell-derived (hUCMSC-Exos) injured endometrium mouse damage model potential mechanisms....
Conventional ovarian tissue cryopreservation often destroys the structural, functional, and DNA integrity of tissue. How to effectively retain ultrastructure subsequent function during has long been an issue concern. Late embryogenesis abundant (LEA) proteins are a class highly hydrophilic have reported protect various cells from water stress. However, whether LEA exert protective effects on remains unknown. To investigate benefit in cryopreservation, we purified recombinant AavLEA1 protein,...