A5002295159- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Bacterial Genetics and Biotechnology
- Pregnancy and preeclampsia studies
- Single-cell and spatial transcriptomics
- Gestational Diabetes Research and Management
- Bariatric Surgery and Outcomes
- Animal Genetics and Reproduction
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- DNA Repair Mechanisms
University of Toronto
2011-2022
Toronto Metropolitan University
2013
Donnelly College
2012
The eukaryotic nucleosome is the fundamental unit of chromatin, comprising a protein octamer that wraps ∼147 bp DNA and has essential roles in compaction, replication gene expression. Nucleosomes chromatin have historically been considered to be unique eukaryotes, yet studies select archaea identified homologs histone proteins assemble into tetrameric nucleosomes. Here we report first archaeal genome-wide occupancy map, as observed halophile Haloferax volcanii. Nucleosome was compared with...
Abstract Systematic analysis of gene overexpression phenotypes provides an insight into function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables highly parallel systematic assessment in pooled cultures. First, constructed genome-level collection ~5100 yeast barcoder strains, each which carries unique barcode, enabling fitness assays with barcode microarray or sequencing readout. Second, open reading frame (ORF) galactose-induced...
ABSTRACT Bacteria of the genus Shigella are a major cause death worldwide (L. von Seidlein et al., PLoS Med. 3:e353, 2006). We sequenced genome flexneri strain M90T Sm (serotype 5a) and compared it to published sequence S. 8401 5b).
By virtue of advances in next generation sequencing technologies, we have access to new genome sequences almost daily. The tempo these is accelerating, promising greater depth and breadth. In light extraordinary advances, the need for fast, parallel methods define gene function becomes ever more important. Collections genome-wide deletion mutants yeasts E. coli served as workhorses functional characterization function, but this approach not scalable, current gene-deletion approaches require...
Genome-wide screening in human and mouse cells using RNA interference open reading frame over-expression libraries is rapidly becoming a viable experimental approach for many research labs. There are variety of gene expression modulation commercially available, however, detailed validated protocols as well the reagents necessary deconvolving genome-scale screens these lacking. As solution, we designed comprehensive platform highly multiplexed functional genetic human, yeast popular,...
By virtue of advances in next generation sequencing technologies, we have access to new genome sequences almost daily. The tempo these is accelerating, promising greater depth and breadth. In light extraordinary advances, the need for fast, parallel methods define gene function becomes ever more important. Collections genome-wide deletion mutants yeasts E. coli served as workhorses functional characterization function, but this approach not scalable, current gene-deletion approaches require...