A5067128912- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Salmonella and Campylobacter epidemiology
- Diet, Metabolism, and Disease
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
University of Central Florida
2023-2024
James Madison University
2021
Metagenomic sequencing analysis is central to investigating microbial communities in clinical and environmental studies. Short-read remains the primary approach for metagenomic research; however, long-read may offer advantages of improved assembly resolved taxonomic identification. To compare relative performance studies, we simulated short- datasets using increasingly complex metagenomes comprising 10, 20, 50 taxa. Additionally, used an empirical dataset paired data generated from mouse...
We have developed and implemented an undergraduate microbiology course in which students isolate, characterize, perform whole genome assembly analysis of Salmonella enterica from stream sediments poultry litter. In the development over three semesters, successive teams collected field samples performed enrichment isolation techniques specific for detection S. enterica. Eighty-eight strains were confirmed using standard microbiological methods PCR invA gene. The isolates' genomes...
Propionic acid (PPA), an anti-fungal agent and common food additive, has been shown to induce atypical neurodevelopment in mice, accompanied by gastrointestinal dysfunction potentially resulting from gut dysbiosis. A putative association between dietary PPA exposure dysbiosis is suggested but not explored directly. Here, we investigated PPA-associated alteration microbial composition that may result Using long-read metagenomic sequencing, microbiomes of mice fed untreated (n=9) or PPA-rich...
Abstract Metagenomic sequencing analysis is central to investigating microbial communities in clinical and environmental studies. Short read remains the primary data type for metagenomic research, however, long promises advantages of improved assembly resolved taxonomic identification. To assess comparative performance short analysis, we simulated datasets using increasingly complex metagenomes comprised 10, 20, 50 taxa. In addition, an empirical dataset paired from mouse fecal pellets was...