A5103262568- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- COVID-19 diagnosis using AI
- Artificial Intelligence in Healthcare and Education
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Artificial Intelligence in Healthcare
- Pneumonia and Respiratory Infections
Cornell University
2025
Weill Cornell Medicine
2025
Institute of Microbial Technology
2017-2023
The ability to sense acid stress and mount an appropriate adaptive response by Mycobacterium tuberculosis, which adapts a long-term residence in the macrophage phagosome, remains one of critical features that defines mycobacterial physiology its intracellular location. To understand mechanistic basis adaptation pathogen, we studied global regulation M. tuberculosis gene expression stress. Although recent studies indicate role for virulence-associated phoP locus pH-driven adaptation, this...
Abstract Mycobacterium tuberculosis (Mtb), a leading cause of death from infection, completes its life cycle entirely in humans except for transmission through the air. To begin to understand how Mtb survives aerosolization, we mimicked liquid and atmospheric conditions experienced by before after exhalation using model aerosol fluid (MAF) based on water-soluble, lipidic cellular constituents necrotic lesions. MAF induced drug tolerance Mtb, remodeled transcriptome protected dying...
Mycobacterium tuberculosis (Mtb), a leading cause of death from infection, completes its life cycle entirely in humans except for transmission through the air. To begin to understand how Mtb survives aerosolization, we mimicked liquid and atmospheric conditions experienced by before after exhalation using model aerosol fluid (MAF) based on water-soluble, lipidic, cellular constituents necrotic lesions. MAF induced drug tolerance Mtb, remodeled transcriptome, protected dying microdroplets...
PhoP-PhoR, one of the 12 two-component systems (TCSs) that empower M . tuberculosis to sense and adapt diverse environmental conditions, remains essential for virulence, therefore, represents a major target develop novel anti-TB therapies. Although both PhoP PhoR have been structurally characterized, signal(s) this TCS responds unknown. Here, we show is sensor acidic pH/high salt which subsequently activate via phosphorylation. In keeping with this, transcriptomic data uncover pH- inducible...
Mycobacterium tuberculosis retains the ability to establish an asymptomatic latent infection. A fundamental question in mycobacterial physiology is understand mechanisms involved hypoxic stress, a critical player persistence. Here, we show that virulence regulator PhoP responds hypoxia, dormancy signal, and effectively integrates hypoxia with nitrogen metabolism. We also provide evidence demonstrate both under limiting conditions during phoP locus controls key genes Consistently, ΔphoP...
A hallmark feature of Mycobacterium tuberculosis pathogenesis lies in the ability pathogen to survive within macrophages under a stressful environment. Thus, coordinated regulation stress proteins is critically important for an effective adaptive response M. tuberculosis, failure which results elevated immune recognition tubercle bacilli with reduced survival during chronic infections. Here, we show that virulence regulator PhoP impacts global heat shock proteins, protect against generated...
Abstract PhoP-PhoR empowers M. tuberculosis to adapt diverse environmental conditions, and remains essential for virulence. Although PhoP PhoR have been structurally characterized, the signal(s) that this TCS responds unknown. In study, we show is a sensor of acidic pH/high salt which activate via phosphorylation. Transcriptomic studies uncover pH-inducible expression regulon significantly inhibited in PhoR-deleted . Using genome-wide screening further identify non-canonical mechanism...
This study focuses on the potential application of Artificial Intelligence (AI) in healthcare and hospitals to improve quality services for patients. The research objectives include investigation existing AI use cases healthcare, exploration areas which can best be applied, identification challenges successful application. utilizes both primary secondary data sources investigate hospitals. is collected through published papers, technical reports, industry news gain an understanding current...
ABSTRACT Mycobacterium tuberculosis (Mtb) retains the unique ability to establish an asymptomatic latent infection. A fundamental question in mycobacterial physiology is understand mechanisms involved hypoxic stress, a critical player persistence. Here, we show that virulence regulator PhoP responds hypoxia, dormancy signal and effectively integrates hypoxia with nitrogen metabolism. We also provide evidence demonstrate both under limiting conditions during phoP locus controls key genes...