A5002404417- Cellular transport and secretion
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- Salivary Gland Disorders and Functions
- Cancer-related molecular mechanisms research
- Healthcare and Venom Research
- Mesenchymal stem cell research
- Bee Products Chemical Analysis
- Lipid Membrane Structure and Behavior
- Biochemical effects in animals
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Sulfur Compounds in Biology
- Redox biology and oxidative stress
- Lipid metabolism and biosynthesis
- Immunotherapy and Immune Responses
- Alcohol Consumption and Health Effects
- Antimicrobial Peptides and Activities
- Pancreatic function and diabetes
- Skin and Cellular Biology Research
- CAR-T cell therapy research
- Caveolin-1 and cellular processes
Asahi University
2014-2024
Mizuho (Japan)
2014-2021
The mechanisms of immunomodulation by mesenchymal stromal cells remain poorly understood. In this study, the effects mouse adipose tissue-derived (ASCs) on spleen alloreactively stimulated anti-CD3 and anti-CD28 antibody-coated (anti-CD3/CD28) beads were observed. Production interferon-γ anti-CD3/CD28 bead-stimulated was significantly suppressed in co-culture with ASCs. However, an augmented intensity CD69 not presence immunosuppressive ASCs partially mediated one or more soluble factors...
To elucidate the genetic events that occur during development of OSCC, present study established a model oral malignancy using mouse squamous cell carcinoma (OSCC) Sq‑1979 line. cells were implanted into syngeneic C3H mice. Subsequently, 233 and metastatic sub‑clones (L cells) from primary as well metastasized lymph node tissues Sq‑1979‑implanted mice established. Compared with parental cells, majority L exhibited higher proliferation rate transplantability, conferred lower survival on...
Numerous studies have shown that the sustained release of ions from dental restorative materials acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, behavior bioresponsiveness released surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types media based on human pulp-derived stem cell (hDPSC) responses. The hDPSCs cultured for 1-7 days S-PRG eluates diluted with varying amounts culture media. several ions,...
We investigated the significance of plasma membrane lipid composition in exocytosis an vitro interaction system using intact secretory granular fraction (SG) isolated from rat parotid gland. When various liposomes (as a model membranes) were added to this assay system, rapid and transient amylase release SG was evoked increased by Ca2+ concentration-dependent manner. The extent depended upon not only amount but also their composition. addition 1,2-diacylglycerol phosphatidic acid egg yolk...
We characterized the Ca2+-independent, membrane-associated phospholipase A2 (PLA2) from rat parotid secretory granules. Among four phosphatidylcholine species with different fatty acyl (palmitoyl, oleoyl, linoleoyl, and arachidonoyl) groups at sn-2 position, 2-arachidonoyl-phosphatidylcholine was preferred substrate. Such specificity also apparent even when coexisted another species. The various well-documented inhibitors of PLA2s, bromoenol lactone, arachidonyl trifluoromethyl ketone,...
Background: We have already reported that mouse Oral Squamous Carcinoma Cells (OSCCs) Sq-1979 specifically enhance the immunosuppressive activity of mesenchymal 10T1/2 cells via functional soluble factor (s). Objective: In this report, we attempted to identify factor(s) mediating immunosuppression cells. Methods: L5-11 are a variant established from metastatic lymph nodes Sq-1979-implanted mice. Unlike parental cells, however, lack promotion in order cytokine mRNAs expressed but not cDNA...
Aim: The aim of this study is to compare the immunomodulatory effects exerted by primary versus metastasized oral squamous cell carcinoma (OSCC) cells. Methods: Mouse OSCC line Sq-1979, 233 cells established from implanted Sq-1979 cells, and L lymph node tissues Sq-1979-implanted mice were subcutaneously inoculated into later abdominal area syngeneic C3H mice. producing capabilities interferon (IFN)-γ, a Th1 cytokine, interleukin (IL)-10, Th2 anti-CD3 antibody-stimulated spleen investigated...
Lipopolysaccharides (LPS) promote allergic responses to nickel (Ni) both in the sensitization and elicitation steps. In this study, we examine effect of pre-sensitization LPS on occurrence Ni allergy using a mouse model.A 100 mg was injected into C57BL/6J mice intraperitoneally (ip). Three weeks later, were subsequently with 0.3 μ moles dichloride (NiCl2) μg CpG-DNA, which acted as an adjuvant. The repeatedly immunized sulfate (NiSO4), along 300 μl adjuvant, Inject Alum (Pierce, USA). Then...
Aim: The aim of this study was to compare the immunomodulatory effects in tumor milieu mouse oral squamous cell carcinoma (OSCC) cells harboring primary and advanced phenotypes. We established an vitro co-culture system using OSCC cells, spleen mesenchymal stromal cells. Methods: Sq-1979 is line derived from C3H mice; 233-11 were a tumor; L3–5, L5–11, L6–8 lymph node-metastasized 10T1/2 fibroblast mice. co-cultured with anti-CD3 antibody-stimulated presence or absence producing capability...
Administration of bone marrow-derived mesenchymal stem cells (MSCs) is a possible treatment for graft-versus-host disease (GVHD) following allogeneic hematopoietic cell transplantation and other inflammatory conditions. To address the mechanism immunosuppression by MSCs, in particular those derived from adipose tissue (AMSCs), AMSCs were isolated three different mouse strains, suppressive capacity thus obtained to suppress interferon (IFN)-γ generation mixed lymphocyte reaction cultures...
Salivary peroxidase and myeloperoxidase are known to display antibacterial activity against oral microbes, previous indications have pointed the possibility that horseradish (HRP) adsorbs onto membrane of major streptococci, Streptococcus mutans sanguinis (S. sanguinis). However, mechanism interaction between HRP bacterial cell wall component is unclear. Dental plaques containing salivary glycoproteins extracellular microbial products visualized with 'dental plaque disclosing agent',...