A5044445276- HIV Research and Treatment
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Reproductive tract infections research
- Mosquito-borne diseases and control
- Urinary Tract Infections Management
- Viral Infections and Vectors
- Cervical Cancer and HPV Research
- Food Allergy and Anaphylaxis Research
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Antimicrobial Peptides and Activities
- Reproductive Health and Contraception
- T-cell and B-cell Immunology
- Pelvic floor disorders treatments
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Immune Response and Inflammation
- Mast cells and histamine
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- interferon and immune responses
- Asthma and respiratory diseases
Regeneron (United States)
2023-2024
Rutgers, The State University of New Jersey
2013-2023
Rutgers New Jersey Medical School
2015-2023
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021
Rutgers Health
2013-2014
Understanding the phenotypic and transcriptional signature of immunoglobulin E (IgE)–producing cells is fundamental to plasma cell (PC) biology development therapeutic interventions for allergy. Here, using a mouse model intranasal house dust mite (HDM) exposure, we showed that short-lived IgE PCs emerge in lung draining lymph nodes (dLNs) during early exposure (<3 weeks) long-lived accumulate bone marrow (BM) with prolonged (>7 weeks). had distinct surface gene expression profiles...
Immunoglobulin E (IgE) is a key driver of type 1 hypersensitivity reactions and allergic disorders, which are globally increasing in number severity. Although eliminating pathogenic IgE may be powerful way to treat allergy, no therapeutic strategy reported date can fully ablate production. Interleukin-4 receptor α (IL-4Rα) signaling required for class switching, IL-4Rα blockade gradually reduces, but does not eliminate, IgE. The persistence after due long-lived + plasma cells that maintain...
The use of depot medroxyprogesterone acetate (DMPA), a 3-monthly injectable hormonal contraceptive, is associated with an increased risk HIV acquisition possibly through alteration the vaginal microbiome. In this longitudinal interventional study, we investigated impact DMPA administration on microbiome in Hispanic White and Black women at baseline (visit 1), 1 month 2), 3 months 3) following treatment by using 16S rRNA gene sequencing. No significant changes were observed after when...
Estrogen has been shown to increase resistance HIV/SIV transmission by increasing the thickness of genital epithelium. The immunological role estrogen in HIV infection primary target cells is less well characterized. We have found that macrophages are a for anti-HIV activity 17β-estradiol (E2). E2 did not affect surface expression CD4 and co-receptors nor attachment monocyte-derived (MDMs). In addition, treatment blocked co-receptor-independent HIV-1VSV-G pseudotyped virus. Quantitative...
IFN-ε is a unique type I IFN that not induced by pattern recognition response elements. constitutively expressed in mucosal tissues, including the female genital mucosa. Although direct antiviral activity of was thought to be weak compared with IFN-α, controls Chlamydia muridarum and herpes simplex virus 2 mice, possibly through modulation immune response. We show here induces an state human macrophages blocks HIV-1 replication. had little or no protective effect activated CD4+ T cells...
CD4 T cells are crucial for the establishment and dissemination of HIV in mucosal tissues during acute infection. Studies indicate that integrin α4β7 preferentially infected by vitro SIV The is thought to promote capture target cells; however, role transmission remains controversial. In this study, we characterized immune phenotypes human cervical examined preference cells. all-trans retinoic acid-differentiated peripheral (atRA-differentiated cells) were included as a comparison.In both...
Background We have previously shown that human defensin 5 (HD5) promotes HIV infectivity in both primary CD4+ T cells and HeLa expressing CD4 CCR5. HD5 is induced response to sexually transmitted infections (STIs) such as Chlamydia trachomatis Neisseria gonorrhoeae, suggesting it plays a role STI-mediated enhancement of transmission. In contrast our findings, recent study reports has an anti-HIV effect under serum-deprived conditions. To resolve these apparently contradictory observations,...
Abstract Interferon ɛ (IFNɛ) is a unique type I IFN that has been implicated in host defense against sexually transmitted infections. Zika virus (ZIKV), an emerging pathogen, can infect the female reproductive tract (FRT) and cause devastating diseases, particularly pregnant women. How IFNɛ contributes to protection ZIKV infection vivo unknown. In this study, we show plays critical role vaginal mice. We found was expressed not only by epithelial cells FRT but also immune stromal at baseline...
Abstract Depot medroxyprogesterone acetate (Depo-Provera) has been associated with an increased risk of HIV acquisition. In a longitudinal study, we investigated the impact Depo-Provera use by healthy women on expression immune markers for preference and infection ex vivo at baseline (visit 1), 1 mo 2), 3 3) after treatment. We found significant increase in frequency integrin α4β7 CD4+ T cells visit 2. Interestingly, Hispanic but not black exhibited cell numbers levels 2, whereas change CCR5...
Depot medroxyprogesterone acetate (Depo-Provera) is the most commonly used injectable hormone contraceptive in Sub-Saharan Africa where HIV incidence high. We determined impact of Depo-Provera on cervical immune cells and mediators healthy women. In this longitudinal study, vaginal, endocervical, rectal swabs were collected at baseline (visit 1), 1 month 2), 3 months 3) after injection. Cervical by cytobrush markers CD4 T analyzed multicolor flow cytometry three different visits. The levels...
Interferonε (IFNε) is a unique type I IFN that has distinct functions from IFNα/β. IFNε constitutively expressed at mucosal tissues, including the female genital mucosa, and reported to be modulated by estrogen seminal plasma. However, its regulation cytokines, TNFα, IL-1β, IL-6, IL-8, IL-17, IL-22 IFNα, which are commonly present in not well documented freshly isolated primary cervical cells tissues. We determined effect of these cytokines on gene expression IFNs an immortalized...
Background: CD4+ T cells, the principal target in acute SIV and HIV infection, are crucial for establishment dissemination of infection mucosal tissues. Studies indicate that α4β7 cells preferentially infected by vitro during infection. The integrin is thought to promote capture cells; however, role transmission remains controversial. In this study, we characterized immune phenotypes human cervical examined preference α4β7+ cells. all-trans retinoic acid differentiated peripheral (at-RA...
Abstract Depo-Provera (Depot medroxyprogesterone acetate, MPA) is associated with an increased risk of HIV acquisition; however, its impact on the cervicovaginal immune milieu and infectivity ex vivo not well characterized. In a longitudinal study, we investigated use by healthy women levels cervical mediators, expression markers for preference (CCR5, integrin a4b7, CD38) CD4+ T cells, at baseline (visit 1), one month 2) three months 3) after treatment. Forty-seven who met eligibility...
Abstract CD4+ T cells, the principal target in acute SIV and HIV infection, are crucial for establishment dissemination of infection mucosal tissues. Studies indicate that α4β7 cells preferentially infected by vitro during infection. Integrin is thought to promote capture cells; however, role integrin transmission remains controversial. In this study, we characterized immunological profiles human cervical examined preference α4β7+ cells. all-trans retinoic acid differentiated peripheral were...
Abstract Interferon epsilon (IFNε), a type-I IFN, is hormonally regulated and plays protective role against several viral bacterial pathogens. In this study, we examined the effect of IFNε on HIV infection its underlying mechanism. at 10ng/mL inhibited human monocyte derived macrophages (MDMs) but not activated CD4+ T cells. Pretreatment MDMs with protected by replication competent X4, R5, X4R5 dual-tropic viruses transmitted/founder viruses. We further delineated mechanism anti-HIV activity...
Interferon ε (IFNε) is a unique type I IFN that has been implicated in host defense against sexually transmitted infections (STIs). Zika virus (ZIKV), an emerging pathogen, can infect the female reproductive tract (FRT) and cause devastating diseases, particularly pregnant women. How IFNε contributes to protection ZIKV infection vivo unknown. Here, we show plays critical role vaginal mice. We found was expressed not only by epithelial cells FRT, but also certain immune other at baseline or...
Abstract Interferon epsilon (IFNe) is a unique type I IFN that not induced by pattern-recognition response elements. IFNɛ constitutively expressed in mucosal tissues including the female genital mucosa. Although direct anti-viral activity of IFNe was thought to be weak compared IFNa, controls Chlamydia muridarum and herpes simplex virus 2 mice, possibly through modulation immune response. We show here induces an antiviral state human macrophages blocks HIV-1 replication. had little or no...
Abstract Interferon e (IFNe) is a critical innate immune mediator that protects the host against sexually transmitted infections. Although current paradigm IFNɛ type I IFN and signals through IFNa receptors (IFNARs), our other published results show has unique gene regulatory functions are distinct from IFNa. constitutively expressed in mucosa, highly abundant female reproductive tract. It exhibits superior mucosal activity compared to IFNa/b. We have previously shown human IFNe induces an...