A5069880049- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Biochemical Acid Research Studies
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Click Chemistry and Applications
- Radical Photochemical Reactions
Prozomix (United Kingdom)
2021-2024
Three enzymatic routes toward γ-hydroxy-α-amino acids by tandem aldol addition–transamination one-pot two-step reactions are reported. The approaches feature an enantioselective addition of pyruvate to various nonaromatic aldehydes catalyzed trans-o-hydroxybenzylidene hydratase-aldolase (HBPA) from Pseudomonas putida. This affords chiral 4-hydroxy-2-oxo acids, which were subsequently enantioselectively aminated using S-selective transaminases. transamination processes investigated involving...
Radical hydrofunctionalizations of electronically unbiased dienes are challenging to render regioselective, because the products nearly identical in energy. Here, we report two engineered FMN-dependent "ene"-reductases (EREDs) that catalyze regiodivergent hydroalkylations cyclic and linear dienes. While previous studies focused exclusively on stereoselectivity alkene hydroalkylation, this work highlights EREDs can control regioselectivity hydrogen atom transfer, providing a method for...
Although optical pure amino alcohols are in high demand due to their widespread applicability, they still remain challenging synthesize, since commonly elaborated protection strategies required. Here, a multi-enzymatic methodology is presented that circumvents this obstacle furnishing enantioenriched 1,3-amino out of commodity chemicals. A Type I aldolase forged the carbon backbone with an aldol motif, which was subsequently subjected enzymatic transamination. panel 194 TAs tested on diverse...
Chiral 2-hydroxy acids and 2-hydroxy-4-butyrolactone derivatives are structural motifs often found in fine commodity chemicals. Here, we report a tandem biocatalytic stereodivergent route for the preparation of these compounds using three stereoselective aldolases two stereocomplementary ketoreductases simple achiral starting materials. The strategy comprises (i) aldol addition reaction 2-oxoacids to aldehydes from E. coli, 3-methyl-2-oxobutanoate hydroxymethyltransferase (KPHMT Ecoli ),...
Abstract ATAs engineered for having an enlarged small binding pocket were applied the synthesis of enantiomerically pure ( R )‐benzo[1,3]dioxol‐5‐yl‐butylamine, a chiral component human leukocyte elastase inhibitor DMP 777 (L‐694,458). Kinetic resolution racemic amine was performed by using L59A variant S )‐selective ATA from Chromobacterium violaceum Cv ‐ATA), providing residual )‐enantiomer in excellent yield and >99% ee . At moderate enzyme loading absence co‐solvent, high volumetric...
Abstract We described a strategy for the enzymatic synthesis of 1‐deoxy and 1,2‐deoxyketoses from aliphatic α‐ketoacids, pyruvate 2‐oxobutyrate, as donors natural aldoses variable chain length acceptors, catalyzed by thermostable transketolase variants Geobacillus stearothermophilus (TK gst ). Analytical studies have been carried out on panel TK with appropriate substrates allowing to select best combinations apply it preparative scale obtained good excellent isolated yields (61%–86%). To...
Abstract Chiral 2‐hydroxy‐4‐arylbut‐3‐enoic acid derivatives are important precursors for the synthesis of angiotensin converting enzyme (ACE) inhibitors, such as enalapril, lisinopril, cilapril or benazepril. In this work, we take advantage unexplored promiscuous ketoreductase activity Δ 1 ‐piperidine‐2‐carboxylate/Δ ‐pyrroline‐2‐carboxylate reductase from Pseudomonas syringae pv. tomato DSM 50315 (DpkA Psyrin ) ( S , E )‐2‐hydroxy‐4‐arylbut‐3‐enoic acids. The strategy was designed an...