A5093895757- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
Dana-Farber Cancer Institute
2024
Harvard University
2024
Deciphering the context-specific relationship between sequence and function is a major challenge in genomics. Existing tools for inducing locus-specific hypermutation evolution native genome context are limited. Here we present programmable platform long-range, called helicase-assisted continuous editing (HACE). HACE leverages CRISPR-Cas9 to target processive helicase-deaminase fusion that incurs mutations across large (>1000–base pair) genomic intervals. We applied identify...
Abstract A major challenge in human genomics is to decipher the context specific relationship of sequence function. However, existing tools for locus hypermutation and evolution native genome are limited. Here we present a novel programmable platform long-range, locus-specific called helicase-assisted continuous editing (HACE). HACE leverages CRISPR-Cas9 target processive helicase-deaminase fusion that incurs mutations across large (>1000 bp) genomic intervals. We applied identify MEK1...