A5075484360- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Antimicrobial Resistance in Staphylococcus
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- Bacterial Identification and Susceptibility Testing
- Chromatin Remodeling and Cancer
- Genetics and Neurodevelopmental Disorders
- DNA and Nucleic Acid Chemistry
- Bacterial biofilms and quorum sensing
- Genetic factors in colorectal cancer
- Polyomavirus and related diseases
- Mitochondrial Function and Pathology
Dhaka Shishu Hospital
2023
Kyoto University
2015-2022
Significance BRCA1 plays a key role in homology-directed repair (HDR) S/G 2 -phase cells. It remains unclear why mutation carriers develop cancer predominantly breast and ovarian tissues. We revealed that physiological concentration (10 nM) of estrogens efficiently induce TOP2β-dependent DSBs the absence cells arrested G 1 phase. This genotoxicity was confirmed also 0 /G epithelial mouse mammary glands. These findings indicated contributes to DSB independent HDR. Our data suggested promotes...
Smarcal1 is a SWI/SNF-family protein with an ATPase domain involved in DNA-annealing activities and binding site for the RPA single-strand-DNA-binding protein. Although role played by maintenance of replication forks has been established, it remains unknown whether contributes to genomic DNA outside S phase. We disrupted SMARCAL1 gene both chicken DT40 human TK6 B cell lines. The resulting SMARCAL1−/− clones exhibited sensitivity chemotherapeutic topoisomerase 2 inhibitors, just as...
Significance A large number of nucleotides is continuously damaged in every cell. Damaged stall replicative DNA polymerases on template strands. Resulting replication blockage released by two alternative pathways, error-free switching (TS) and error-prone translesion synthesis (TLS). TLS plays a major role converting damage to mutations, thus, the relative usage over TS determines frequency mutagenesis. The controlled post-translational modification proliferating cell nuclear antigen (PCNA),...
Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become a major concern for public health. Compared to infections caused by methicillin-susceptible (MSSA), MRSA causes severe with increased morbidity and mortality also places large economic pressure on our health care sector. Objective: The purpose of the present study was determine prevalence in different clinical samples isolated from admitted patients tertiary hospital, Bangladesh. Methodology: This cross-sectional...
Homologous recombination (HR) repairs DNA double-strand breaks using intact homologous sequences as template DNA. Broken and form joint molecules (JMs), including Holliday junctions (HJs), HR intermediates. HJs are resolved to crossover noncrossover products. A mismatch repair factor, MLH3 endonuclease, produces the majority of crossovers during meiotic HR, but it remains elusive whether factors promote in nonmeiotic cells. We disrupted genes encoding PMS2 endonucleases human B cell line,...
Chain-terminating nucleoside analogs (CTNAs), which cannot be extended by DNA polymerases, are widely used as antivirals or anti-cancer agents, and can induce cell death. Processing of blocked ends, like camptothecin-induced trapped-topoisomerase I, mediated TDP1, BRCA1, CtIP MRE11. Here, we investigated whether the CtIP-BRCA1 complex MRE11 also contribute to cellular tolerance CTNAs, including 2',3'-dideoxycytidine (ddC), cytarabine (ara-C) zidovudine (Azidothymidine, AZT). We show that...
ATM gene mutation carriers are predisposed to estrogen-receptor-positive breast cancer (BC). prevents BC oncogenesis by activating p53 in every cell; however, much remains unknown about tissue-specific after loss. Here, we report that controls the early transcriptional response estrogens. This depends on topoisomerase II (TOP2), which generates TOP2-DNA double-strand break (DSB) complexes and rejoins breaks. When TOP2-mediated ligation fails, facilitates DSB repair. After estrogen exposure,...
The human genome contains hundreds of large, structurally diverse blocks that are insufficiently represented in the reference and thus not amenable to genomic analyses. Structural diversity population suggests these unstable germline; however, whether or also cancer remains elusive. Here we report 500 kb block called KRTAP_region_1 (KRTAP-1) on 17q12-21 recurrently demarcates amplicon ERBB2 (HER2) oncogene breast tumors. KRTAP-1 carries numerous tandemly-duplicated segments exhibit within...
Background: Staphylococcus aureus, mainly a hospital acquired infection is responsible for many suppurative lesions and has demonstrated the ability of developing resistance to antimicrobial agents leading life threatening infections long stay. Objective: This study was aimed determine prevalence antibiotic susceptibility profiles aureus in different clinical specimens isolated from admitted patients. Methodology: cross-sectional conducted 1 January 2019 31 December Microbiology Department...