A5041638471- HIV Research and Treatment
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Diabetes and associated disorders
- IL-33, ST2, and ILC Pathways
Karolinska Institutet
2020-2025
University Medical Center Utrecht
2024
Lomonosov Moscow State University
2020
HIV broadly neutralizing antibodies (bnAbs) can suppress viremia and protect against infection. However, their elicitation is made difficult by low frequencies of appropriate precursor B cell receptors the complex maturation pathways required to generate bnAbs from these precursors. Antibody genes be engineered into cells for expression as both a functional antigen receptor on surfaces secreted antibody. Here, we show that bnAb-engineered primary mouse adoptively transferred vaccinated in...
HIV broadly neutralizing antibodies (bnAbs) can suppress viremia and protect against infection 1 . However, their elicitation is made difficult by low frequencies of appropriate precursor B cell receptors the complex maturation pathways required to generate bnAbs from these precursors 2 Antibody genes be engineered into cells for expression as both a functional receptor on surfaces secreted antibody 3–5 Here, we show that bnAb-engineered primary mouse adoptively transferred vaccinated in...
<title>Abstract</title> The continued evolution of SARS-CoV-2 underscores the need to understand qualitative aspects humoral immune response elicited by spike immunization. Here, we combined monoclonal antibody (mAb) isolation with deep B cell receptor (BCR) repertoire sequencing rhesus macaques immunized prefusion-stabilized glycoprotein. Longitudinal tracing spike-sorted lineages in multiple compartments demonstrated increasing somatic hypermutation and broad dissemination vaccine-elicited...
Abstract Objectives The caecum bridges the small and large intestine plays a front‐line role in discriminating gastrointestinal antigens. Although dysregulated acute chronic conditions, tissue is often overlooked immunologically. Methods To address this issue, we applied single‐cell transcriptomic‐V(D)J sequencing to FACS‐isolated CD45 + caecal patch/lamina propria leukocytes from healthy (5‐year‐old) female rhesus macaque ex vivo coupled these data VDJ deep reads haematopoietic tissues....
Abstract The continued evolution of SARS-CoV-2 underscores the need to understand qualitative aspects humoral immune response elicited by spike immunization. Here, we combine monoclonal antibody (mAb) isolation with deep B cell receptor (BCR) repertoire sequencing rhesus macaques immunized prefusion-stabilized glycoprotein. Longitudinal tracing spike-sorted lineages in multiple compartments demonstrates increasing somatic hypermutation and broad dissemination vaccine-elicited cells draining...
Summary Protective antibodies against HIV-1 require unusually high levels of somatic hypermutations (SHMs) introduced in germinal centers (GCs). To achieve this, a sequential vaccination approach was proposed. Using antibody knock-in mice with fate-mapping genes, we examined if antigen affinity affects the outcome B cell recall response. Compared to boost, low boost resulted decreased numbers memory-derived cells secondary GCs, but higher average SHM, indicating an threshold for memory enter...