A5111793017- Birth, Development, and Health
- Carcinogens and Genotoxicity Assessment
- Nerve injury and regeneration
- Effects and risks of endocrine disrupting chemicals
- Assisted Reproductive Technology and Twin Pregnancy
- Neonatal Respiratory Health Research
- Pharmaceutical studies and practices
- Pluripotent Stem Cells Research
- Pregnancy and preeclampsia studies
- Animal testing and alternatives
- Chemical Reactions and Isotopes
- Neurogenesis and neuroplasticity mechanisms
- Animal Genetics and Reproduction
- Connective tissue disorders research
- Marine Invertebrate Physiology and Ecology
- Pregnancy and Medication Impact
- Neuropeptides and Animal Physiology
- Science, Research, and Medicine
- Reproductive Biology and Fertility
- Folate and B Vitamins Research
- Ion channel regulation and function
- Pesticide Exposure and Toxicity
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Insect and Arachnid Ecology and Behavior
University of Alabama
1978-2024
Public Health England
2020
University of Exeter
2020
Medical Research Council
2020
Abt Global (United States)
2009
Wake Forest University
2008
University of Washington Medical Center
2005
Washington University in St. Louis
1986-2003
University of Washington
2001-2002
Jefferson College
1981-1997
We have discovered that axotomy of sciatic nerve induces Schwann cells distal to the lesion express de novo, or at greatly increased levels, receptors for growth factor (NGF). Surgical transection was performed on adult Sprague-Dawley rats, and, various times after operation, following tissues were dissected quantitation NGF receptor: L4 and L5 dorsal root ganglia, proximal transection, tibialis anterior muscle, skin dorsum foot. The receptor content these samples determined by labeling...
Programmed cell death (apoptosis) is a normal process in the developing nervous system. Recent data suggest that certain features seen of programmed may be favored versus adult brain response to different injuries. In well characterized model neonatal hypoxia-ischemia, we demonstrate marked but delayed which there prominent DNA laddering, TUNEL-labeling, and nuclei with condensed chromatin. Caspase activation, required many cases apoptotic death, also followed time course after...
We have developed a method to immunoprecipitate rat nerve growth factor (NGF) receptor proteins and applied the detect NGF molecules in brain. Crosslinking 125I-labeled either PC12 cells or cultured sympathetic neurons yielded two radiolabeled (90 kDa 220 kDa) that were immunoprecipitated by monoclonal antibody 192-IgG. Further, 192-IgG precipitated proteins, with expected sizes (80 210 of noncrosslinked components, from among numerous surface-iodinated cell proteins. These results...
We have demonstrated in vitro and vivo the specific binding of a monoclonal antibody to rat nerve growth factor (NGF) receptor. Previous work had shown that this antibody, designated 192-IgG, does not compete with NGF for receptor PC12 cells, but instead interacts increase cells (Chandler, C. E., L. M. Parsons, Hosang, E. Shooter, 1984, J. Biol. Chem., 259:6882-6889). In present study, solid-phase separation assay verified formation ternary complex receptor, NGF: 125I-labeled 192-IgG...
Injury to the peripheral processes produces a profound cell loss (40-50%) in dorsal root ganglion of newborn rats. Although division central little or no cellular change sensory adult animals, information has been available on effect section developing ganglion. We show that 6 days after rhizotomy rats, there is 50% decrease neuronal number L5 A combined and lesion process results greater (70%). Both these effects can be prevented by concomitant treatment with nerve growth factor. also...
By a uniformly applied protocol, adult hydra are exposed to test substance over broad range of concentrations, and the minimal toxic concentration is determined within one-tenth log. In second experiment, dissociated cells manipulated into configuration wherein, if undisturbed, they will achieve developmental events characteristic any embryo undergo total whole-body regeneration. During its 4-day ontogenesis this artificial "embryo" by same protocol as developmentally The ratio (A) (D)...
ABSTRACT Oral administration of 60,000 international units vitamin A acetate to pregnant rats for 3 consecutive days beginning on either day 9 or 10 pregnancy resulted in more than 80 per cent, their embryos developing cleft palate. The initiation palatine shelf movement normal control was associated with an aggregation preosteoblastic tissue the medial aspect vertically oriented shelves at junction anterior two-thirds and posterior one-third. Delay from vertical horizontal position not...
Methods are described for the thiophosphorylation and recovery of histones non-histone proteins modified by kinases in uitro isolated cell nuclei.The ATP analogue adenosine 5'-0-(3-thiotriphosphate) is shown to be an effective donor thiophosphoryl groups reaction catalyzed cyclic AMPdependent protein kinase calf thymus lymphocytes.Substrates include HI, H2B, H3 high mobility group HMG-1.The modification these permits separation newly molecules affinity chromatography on...
The detection of small concentrations N-nitrosamines in biological materials requires their prior selective extraction and concentration. A procedure is described for the separation both volatile non-volatile as a class, subsequent determination at low levels. N-Nitrosamines are adsorbed from aqueous solution on to activated carbon which many can be desorbed varying extents by refluxing with methanol. Two sensitive techniques used separated N-nitrosamines, namely differential polarography...
Abstract Female Long‐Evans rats, were given a purified PGA‐deficient diet containing 10 mg% of the antimetabolite, 9‐methyl‐PGA, from days 8 to gestation, followed by PGA‐supplemented day autopsy. Thisregimen resulted in 18% embryonic death 11, 65% 12, and 100% 13 pregnancy. No morphological differences between control embryos observed at nine or one‐half but ten‐day showed retardation growth development decreased mitosis, especially neural epithelium. In 11‐day cranial portion tube was...
A large and diverse group of chemicals was evaluated in the Hydra assay, outcomes were compared with those standard developmental toxicity evaluations using data from pregnant mammals. correctly identified each substances previously found by vivo tests to be uniquely hazardous utero development. Its overall accuracy over 90%, all its errors false positives. The fact that these positives indeed could have been established subsequently routine testing In no instance did assay indicate a...
Adult hydra and artificial “embryos” composed of randomly reaggregated cells dissociated were exposed to test chemicals. The lowest concentration killing adult became the numerator, disrupting development denominator a ratio. This toxic (A) developmentally (D) or A/D ratio from was compared calculated published reports employing standard laboratory animals. Data rodents considered if both developmental toxicities consistent for: species, route, duration treatment had direct dose-response...