Abstract Organelle‐targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria lysosomes of living cells, respectively. Both exhibited long‐lived phosphorescence, which is sensitive oxygen quenching. The photocytotoxicity was evaluated under normoxic hypoxic conditions. results showed HeLa cells treated with mitochondria‐targeted complex maintained...

A type I Ru(<sc>ii</sc>) photosensitizer retained an excellent PDT effect under hypoxia through the formation of highly-oxidative hydroxyl radicals light irradiation.

Phosphorescent iridium(III) complexes with three bidentate carborane-functionalized ligands have been synthesized. The luminescent efficiency of the o-carborane-based facial complex fac-o shows solvent-molecule dependence both in solution and solid state that is related to dielectric constant. water-soluble high phosphorescence-efficient nido nido-fac-o can be used fluorescence lifetime bioimaging.

Molecular oxygen (O 2 ) plays a key role in many physiological processes, and becomes toxicant to kill cells when excited 1 O . Intracellular levels, or the degree of hypoxia, are always viewed as an indicator cancers. Due highly efficient cancer therapy ability low side effect, photodynamic (PDT) one most promising treatments for Herein, early‐stage diagnosis system is reported based on phosphorescent conjugated polymer dots (Pdots) containing Pt(II) porphyrin oxygen‐responsive group...

Oxygen plays a crucial role in many biological processes. Accurate monitoring of oxygen level is important for diagnosis and treatment diseases. Autofluorescence an unavoidable interference luminescent bioimaging, so that amount research work has been devoted to reducing background autofluorescence. Herein, phosphorescent iridium(III) complex‐modified nanoprobe developed, which can monitor concentration also reduce autofluorescence under both downconversion upconversion channels. The...

Monitoring tumor biomarkers is crucial for cancer diagnosis, progression monitoring, and treatment. However, identifying single or multiple with the same spatial locations can cause false-positive feedback. Herein, we integrated DNA tetrahedron (DT) structures logic-responsive signal amplifying capability to construct transmembrane logic nanodevices (TDLNs) in situ sequential imaging of glycoprotein mucin 1 (MUC1) cytoplasmic microRNA-21 (miR-21) cell identifications. The TDLNs were...

Accurate detection and imaging of tumor-related microRNA (miRNA) in living cells hold great promise for early cancer diagnosis prognosis. One the challenges is to develop methods that enable identification multiple miRNAs simultaneously further improve accuracy. Herein, a simultaneous method two was established by using programmable designed DNA tetrahedron nanostructure (DTN) probe includes nucleolin aptamer (AS1411), miRNA capture strands, pairs metastable catalytic hairpins at different...

Abstract Prodrug photolysis enables spatiotemporal control of drug release at the desired lesions. For photoactivated therapy, near-infrared (NIR) light is preferable due to its deep tissue penetration and low phototoxicity. However, most photocleavable groups cannot be directly activated by NIR light. Here, we report a upconversion-like process via only one step energy transfer for light-triggered prodrug photolysis. We utilize photosensitizer (PS) that can singlet-triplet (S-T) absorption...

Abstract Organelle‐targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria lysosomes of living cells, respectively. Both exhibited long‐lived phosphorescence, which is sensitive oxygen quenching. The photocytotoxicity was evaluated under normoxic hypoxic conditions. results showed HeLa cells treated with mitochondria‐targeted complex maintained...

Photochemical reactions at lower energy than the absorption window are currently achieved by multi-photon processes, including two-photon and photon upconversion, which have limited utilization efficiency. Here, we report a one-photon strategy based on triplet-triplet transfer (TTET) between photosensitizer photocleavable molecule to achieve photolysis low energy. To verify this concept, chose platinum(II) tetraphenyltetrabenzoporphyrin (PtTPBP) as synthesized boron-dipyrromethene...

Merocyanines adsorbed into the mesopores of mSiO2 shell NaYF4: 20% Yb, 2% Er, 0.2% Tm nanocrystals are demonstrated as ratiometric upconversion luminescence (UCL) probe for highly selective detection HS(-) in living cells through inhibition energy transfer from UCL to absorbance merocyanines. The has been used sensing H2S with high sensitivity and selectivity.

In this work, we propose a three-layer hierarchical hybridization chain reaction (3L hHCR) composed of 1stHCR, 2ndHCR, and 3rdHCR to achieve robust signal amplification efficiency broaden the applied range HCR-based systems. principle, execution superior HCR generates formation initiator (named as 2ndI or 3rdI) subordinate that relies on introduction target sequence (1stI). To avoid high background 3L hHCR system, strategy "splitting reconstruction" was adopted. The designed two separate...

Carborane-incorporated (at both carbon and boron sites) tribranched fluorophores were efficiently prepared. oB significantly improves the fluorescence quantum yield. mC exhibits largest two-photon absorption among known carborane-based derivatives has been successfully applied in 2D & 3D bioimaging.

An xylenol orange-functionalized upconversion nanoprobe has been developed, which can be used for ratiometric luminescence bioimaging of intracellular pH changes.

Phosphoreszierende IrIII-Komplexe mit drei carboranfunktionalisierten zweizähnigen Liganden wurden synthetisiert. Die Lumineszenz des facial koordinierten Komplexes fac-o o-Carboran-Substituenten hängt in Lösung und im Festkörper vom Vorhandensein von Lösungsmittelmolekülen ab. Der wasserlösliche, stark phosphoreszierende nido-Komplex nido-fac-o kann biologischen Fluoreszenzverfahren genutzt werden.

A selective phosphorescent biothiols probe is synthesized based on Ir(III) complex 1, which has 2,2'-biquinoline as the N^N ligand for realizing satisfied two-photon absorption cross-section and two-functionalized 2-phenylpyridine ligands with an α,β-unsaturated ketone moiety thiol reaction site. The one- optical properties of 1 are investigated through UV-vis spectrum photoluminescence spectrum. This can act excellent excited "OFF-ON" 1,4-addition biothiol to ketones. Moreover,...

An endoperoxide-containing polymeric carrier was developed to realize the photothermal-triggered release of singlet oxygen for killing cancer cells.

MicroRNAs (miRNAs) are found in extremely low concentrations cells, so highly sensitive quantitation is a great challenge. Herein, simple dual-amplification strategy involving target-activated catalytic hairpin assembly (CHA) coupled with multiple fluorophores concentrated on one X-shaped DNA reported. In this strategy, four probes (H1, H2, H3, and H4) modified FAM BHQ1 at both sticky ends, while circulating probe (H0) used to activate CHA circuits once it binds complementary sequences the...

Rational design the energy bridge of organic ligands in scintillating lanthanide coordination polymers improved conversion efficiency to enhance characteristic emission cations, achieving high efficient X-ray imaging.

Challenges remained in precisely real-time monitoring of apoptotic molecular events at the subcellular level. Herein, we developed a new type intelligent DNA biocomputing nanodevices (iDBNs) that responded to mitochondrial microRNA-21 (miR-21) and microRNA-10b (miR-10b) simultaneously which were produced during cell apoptosis. By hybridizing two hairpins (H1 H2) onto nanospheres (DNSs) had been previously modified with mitochondria-targeted triphenylphosphine (TPP) motifs, iDBNs assembled...

Abstract 2‐Thienylpyridyl iridium(III) complexes containing an o ‐, m or p ‐carboranylvinyl‐2,2′‐bipyridine ligand and various counteranions (denoted ‐ PF 6 , BF 4 SbF ClO OTf NO 3 BPh F Cl ) were synthesized by using C ‐formyl carboranes as starting materials. The solid‐state structures of showed that the cations form twisted cavities in which anions are fixed multiple hydrogen bonds. Anion–hydrogen interactions investigated for nine ‐carborane‐based with different counteranions. All...

Nonalcoholic fatty liver disease (NAFLD) can progress gradually to failure, early warning of which is critical for improving the cure rate NAFLD. In situ imaging and monitoring overexpressed miR-21 an advanced strategy NAFLD diagnosis. However, this usually suffers from high background in living cells owing complexity biological system. To overcome problem, herein, we have developed a one-donor-two-acceptor nanoprobe by assembling gold nanoparticles (AuNPs) coupled with BHQ2 (AuBHQ) quantum...

Photoresponsive drug release systems can enhance accumulation at the sites where light is applied. Nowadays, photocleavable groups used in usually require ultraviolet or blue irradiation, which limits tissue penetration depth and harmful to normal cells living bodies. A one-photon upconversion-like photolysis strategy, cleave green light-activatable prodrugs with red presence of a light-excitable photosensitizer organic solvents, developed. However, both prodrug are hydrophobic their energy...

Sensitive sensing is critical when developing new calculation systems with weak input signals (ISs). In this work, a "weak-inputs-strong-outputs" strategy was proposed to guide the construction of sensitive logic nanodevices by coupling an input-induced reversible DNA computing platform hybridization chain reaction-based signal amplifier. By rational design sequence elements (CEs) so as avoid cross-talking between ISs and amplifier, newly formed have good sensitivity even at low...