A5092464477- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Sensors
- Planarian Biology and Electrostimulation
University of Toronto
2023-2025
Ted Rogers Centre for Heart Research
2023-2025
Biological barriers formed by the endothelium and epithelium regulate nutrient exchange, disease development, drug delivery. Organ-on-chip (OOC) systems effectively model these incorporating key biophysical cues like microscale...
Cell culture models of endothelial and epithelial barriers typically use porous membrane inserts (e.g., Transwell inserts) as a permeable substrate on which barrier cells are grown, often in coculture with other cell types the opposite side membrane. Current methods to characterize function can disrupt or provide bulk measurements that cannot isolate resistance alone. Electrical cell–substrate impedance sensing (ECIS) addresses these limitations, but its implementation has been limited by...
Measurement of endothelial and epithelial barrier integrity is important for a variety in vitro models, including Transwell assays, cocultures, organ-on-chip platforms. Barrier resistance typically measured by trans-endothelial electrical (TEER), but TEER invasive cannot accurately measure isolated monolayer coculture or most devices. These limitations are addressed porous membrane cell–substrate impedance sensing (PM-ECIS), which measures cell monolayers grown directly on permeable...
Abstract Cell culture models of endothelial and epithelial barriers typically use porous membrane inserts (e.g., Transwell inserts) as a permeable substrate on which barrier cells are grown, often in co-culture with other cell types the opposite side membrane. Current methods to characterize function can disrupt or provide bulk measurements that cannot isolate resistance alone. Electrical cell-substrate impedance sensing (ECIS) addresses these limitations but its implementation has been...
ABSTRACT Conventional trans-endothelial electrical resistance (TEER) setups are invasive and cannot directly measure monolayer integrity in co-culture. These limitations addressed by porous membrane cell-substrate impedance sensing (PM-ECIS), which measures barrier cell monolayers grown on permeable membranes patterned with electrodes. Here we advance the design utility of PM-ECIS investigating its sensitivity to working electrode size correlation TEER. Gold electrodes were fabricated...
Abstract Traditionally, biological barriers are assessed in vitro by measuring trans-endothelial/epithelial electrical resistance (TEER) across a monolayer using handheld chopstick electrodes. Implementation of TEER into organ-on-chip (OOC) setups is challenge however, due to non-uniform current distribution and interference from biomaterials typically found such systems. In this work, we address the pitfalls standard measurement through application porous membrane cell-substrate impedance...