A5084885924- Botulinum Toxin and Related Neurological Disorders
- Toxin Mechanisms and Immunotoxins
- Diphtheria, Corynebacterium, and Tetanus
- Plant-based Medicinal Research
- Angiogenesis and VEGF in Cancer
- Cardiovascular Health and Disease Prevention
- Lymphatic System and Diseases
- Biomarkers in Disease Mechanisms
- Sperm and Testicular Function
- Blood properties and coagulation
- Monoclonal and Polyclonal Antibodies Research
- Nuclear Receptors and Signaling
- GDF15 and Related Biomarkers
- Cardiovascular Disease and Adiposity
- Adipokines, Inflammation, and Metabolic Diseases
- Clostridium difficile and Clostridium perfringens research
- Lipoproteins and Cardiovascular Health
- Nutrition and Health in Aging
- Cardiac Imaging and Diagnostics
- Ion channel regulation and function
- Galectins and Cancer Biology
- Tuberous Sclerosis Complex Research
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
- Adipose Tissue and Metabolism
Kure Medical Center
2013-2024
National Hospital Organization
2013-2023
Osaka University
1989-2008
Jichi Medical University
1991-2000
Novem (Netherlands)
1993
Jikei University School of Medicine
1991
Osaka University Hospital
1989
Sumitomo Hospital
1988
No AccessJournal of Urology1 Mar 1988Surgical Repair Varicocele at Puberty: Preventive Treatment for Fertility Improvement A. Okuyama, M. Nakamura, Namiki, Takeyama, Utsunomiya, H. Fujioka, Itatani, Matsuda, K. Matsumoto, and T. Sonoda OkuyamaA. Okuyama , NakamuraM. Nakamura NamikiM. Namiki TakeyamaM. Takeyama UtsunomiyaM. Utsunomiya FujiokaH. Fujioka ItataniH. Itatani MatsudaM. Matsuda MatsumotoK. Matsumoto SonodaT. View All Author...
Tetanus neurotoxin (molecular weight approximately 160,000) was purified from bacterial extracts (intracellular toxin) and mildly trypsinized culture filtrates (extracellular toxin). Both preparations could be dissociated reversibly into two polypeptide chains, with molecular weights of 53,000 (fragment alpha) 107,000 beta), by treatment 100 mM dithiothreitol (DTT) 4 M urea concomitant loss toxicity. Upon removal DDT the toxin preparation dialysis, these fragments reassociated, forming whole...
<h3>Background:</h3> Obesity is a risk factor for acute pancreatitis (AP), but the molecular mechanism remains unclear. Adiponectin, an adipose tissue-derived secretory factor, has anti-inflammatory properties in addition to various biological functions, and its plasma concentrations are reduced obese subjects. However, role of adiponectin AP not been investigated. <h3>Aim:</h3> To determine effects on AP. <h3>Methods:</h3> We investigated experimental by using adiponectin-knockout (APN-KO)...
We investigated the effects of Bordetella bronchiseptica dermonecrotic toxin on DNA synthesis in MC3T3-E1 cells. The rate [methyl-3H]thymidine incorporation increased toxin-treated cells more than 24 h after addition under serum-starved conditions. This effect was dependent concentration ranging from 0.3 to 3 ng/ml and eliminated by aphidicolin hydroxyurea, inhibitors for replication. In culture, number did not increase but polynucleated appeared their ca. 50% total 6 days addition. From...
A rapid, simplified method for production and purification of tetanus toxin from bacterial extracts was described. The were prepared by stirring young cells (ca. 45-h culture) Clostridium tetani in 1 M NaCl-0.1 sodium citrate, pH 7.5, overnight at 0 to 4 degrees C. purified a combination (i) ammonium sulfate fractionation (0 40% saturation), (ii) ultracentrifugation removal particulate materials, (iii) gel filtration high-pressure liquid chromatography on TSK G3000 SW-type column. This...
A hemolysin, botulinolysin, produced by Clostridium botulinum was purified to homogeneity and characterized. First, a strain of C. type C, C-203 Tox, which large amount selected, optimal culture medium conditions for its production hemolysin were determined. The in the supernatant this under combination ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, Sephadex G-75 gel permeation SP-Toyopearl 650 M cation-exchange with recovery 12%. gave single protein band...
To determine the optimal temperature for DNA, RNA, and protein syntheses in human testis, levels of incorporation 3H-thymidine, l4C-uridine, 14C-leucine into testicular tissue were studied at 28 °C, 31 34°C, 37 °C vitro. The results suggest that DNA synthesis is a maximum 31°C shows delicate sensitivity, whereas RNA are 34 or temperature-dependent. It concluded sensitivity may be one primary causes thermal inhibition spermatogenesis.
Objective: Cardioplegic solutions often cause high blood concentrations of potassium. The conventional hemoconcentration circuit was improved to correct electrolyte imbalances through a method involving dilutional ultrafiltration (DUF) and an alternative hemodialysis (ALTHD) method. This study aimed determine the effectiveness this ALTHD Methods: Bovine used, in conjunction with hemoconcentrator, experimental (HD) evaluate determined by hematocrit measurements following procedure. Results:...
This study clarified the features of a hemoconcentrator-based, alternative hemodialysis (ALTHD) method that improves speed serum potassium (K(+)) concentration adjustments, compared with dilutional ultrafiltration (DUF), during cardiopulmonary bypasses.Standardized bovine blood was recirculated (300 ml/min) through an in vitro hemoconcentrator circuit; hematocrit, K(+) and glucose levels were measured at 5-20 min after DUF or ALTHD. We evaluated dialysis speeds 50-250 ml/min ALTHD 50-1000...
Elimination of cryoprecipitable plasma components (cryogels) by cryofiltration from the circulating blood patients with drug-resistant rheumatoid arthritis (RA) alleviates clinical symptoms including morning stiffness and arthralgia. The cryogels thus isolated were found to consist mainly fibrinogen (Fbg) fibronectin (FN). Analysis immunoblotting an anti-cellular FN monoclonal antibody revealed that cellular (cFN) co-existed (pFN) in derived patients. Using ELISA, we assessed cFN together...
Fragment B, the N-terminal half of heavy chain, an important domain tetanus neurotoxin molecule, was isolated for first time. Tetanus toxin (composed three domains, A, and C) prepared from culture filtrates. A-B, derived treated mildly with papain, used isolation fragment B. A-B obtained dissociated into fragments A B by reduction 100 mM dithiothreitol treatment 2 M urea. separated ion-exchange column chromatography on a Mono Q equilibrated 20 Tris hydrochloride buffer (pH 7.6), containing 1...
We have evaluated the effect of lysine binding sites in krigle structures on activation plasminogen with plasmin and staphylokinase (SAK) complex to SAK. Activation native (Glu-plasminogen) by a catalytic amount plasmin-SAK increased presence ε-amino-n-caproic acid (EACA) then decreased higher concentrations EACA. By contrast, modified (Lys-plasminogen) an EACA-concentration-dependent manner. This decrease was explained more than 10-fold Km for Lys-plasminogen EACA competitive inhibitor K1...
Objective: To analyze the benefits of treatment with Recombinant Human Leptin in areas presenting Lipodystrophy and its impacts on serum levels Glycemia Glycated Hemoglobin. Methods: This is an integrative review, which data collection was conducted PubMed database. Twelve articles were selected from period 2002 to 2020. Results: Treatment through has been studied over years prevent delay onset this alteration. replacement performed individuals Lipodystrophy. Administration hormone...
Abstract Background Galectin-3 is a multifunctional carbohydrate-binding lectin that involved in inflammation, tissue remodeling, and fibrosis. High circulating levels of galectin-3 are associated with all-cause mortality, cardiovascular (CV) and/or major adverse CV events patients diseases such as heart failure coronary artery disease (CAD). However, the associations non-CV mortality cancer suspected or known CAD unknown. Methods Serum were measured 2,418 undergoing elective angiography....