A5069034050- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Wheat and Barley Genetics and Pathology
- Viral gastroenteritis research and epidemiology
- 3D Printing in Biomedical Research
- Erythrocyte Function and Pathophysiology
- Transgenic Plants and Applications
- Enzyme Structure and Function
- Alkaline Phosphatase Research Studies
- Animal Genetics and Reproduction
- Animal Nutrition and Physiology
- Bacterial Genetics and Biotechnology
- Blood properties and coagulation
- Biotin and Related Studies
- Biochemical and Molecular Research
- Phytase and its Applications
- Plant Molecular Biology Research
- Amino Acid Enzymes and Metabolism
- Hemoglobin structure and function
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Plant Reproductive Biology
University of California, Berkeley
2023-2024
Elucid Bioimaging
2023
Fox Chase Cancer Center
1976-1991
We screened a chicken liver cDNA expression library with probe spanning the distal region of vitellogenin II (VTGII) gene promoter and isolated clones for transcription factor that we have named VBP (for gene-binding protein). binds to one most important positive elements in VTGII appears play pivotal role estrogen-dependent regulation this gene. The protein sequence was deduced from nearly full length copy found contain basic/zipper (bZIP) motif. As expected bZIP factor, its target DNA site...
The endogenous chicken vitellogenin II (VTGII) gene is transcribed exclusively in hepatocytes response to estrogen. We previously identified two estrogen elements (EREs) upstream of this gene. now present an analysis the VTGII promoter activated by these EREs Chimeric VTGII-CAT genes were cotransfected into LMH hepatoma cells along with receptor expression vector, and transient CAT was assayed after culturing absence or presence An constructs bearing deletions downstream more proximal ERE...
This report characterizes three components of the fractionated wheat germ protein synthesizing system, factors D1, D2c, and D2d. FActor D1 has a molecular weight about 420,000 is comprised 7-10 varying in mass between 28,000 105,000 daltons. The factor absolutely required for formation both 40-S ribosome.Met-tRNAiMet 80 S complexes except at low Mg2+ concentration, where significant 40 ribosome complex can be obtained without provided reaction products are fixed with glutaraldehyde. Factor...
A factor that binds Met-tRNAiMet in a ternary complex (eucaryotic initiation (eIF2) ) and an auxiliary component strongly stimulates the activity of binding (CoeIF2) have been purified from extracts wheat germ. The molecular weights two factors, as determined by glycerol gradient centrifugation sodium dodecyl sulfate gel electrophoresis, are 88,000 20,000, respectively. Incubation germ eIF2 with gamma-[32P]ATP alpha-subunit kinase results phosphorylation Mr = 40,500 subunit, while no...
Protein synthesis in a resolved homogenate of wheat germ requires ATP and eight factors functioning at the level protein chain initiation. To identify component(s) interacting with ATP, different were treated affinity analogue 5'-p-fluorosulfonylbenzoyladenosine (FSBA) tested for their function synthesis. The activity eukaryotic initiation factor 4A (eIF4A) was strongly curtailed, whereas all other unaffected. At concentration 250 microM, AMP, ADP, protected eIF4A against FSBA inactivation,...
Abstract Wheat germ S100 supernatant was resolved into 10 components, all of which are required for tobacco mosaic virus RNA-directed incorporation amino acid protein. Two the C2b and C2a, are, respectively, elongation factors 1 2, having molecular weights 51,000 72,000. A third factor (C2e) binds Met-tRNA in a GTP-requiring reaction is absolutely formation both 40 S ribosome.Met-tRNAiMet 80 complexes. This considered to be eucaryotic initiation 2. Formation ribosome Met-tRNAiMet complexes...
Phosphoglycolate phosphatases from spinach and human red blood cells show a number of common features not often found in enzymes. 1) Both enzymes are activated more than 50-fold by millimolar concentrations C1-.Other inorganic anions carboxylic acids also activate.2) Each enzyme has limited substrate specificity yet each hydrolyzes P-glycolate ethyl-P with the same maximal velocity.L-P-lactate is only good for cell enzyme. 3)With both initial rate data obtained varying C1give parallel line...
When 32P-glycolate and phosphoglycolate phosphatase from spinach are mixed, 32P is incorporated into acid precipitated protein. Properties that relate the phosphorylation of enzyme to are: Km value for P-glycolate similar protein substrate hydrolysis; in phosphoenzyme diluted by unlabeled or specific alternative substrate, ethyl-P; activator Cl- enhances effectiveness ethyl-P as a an inhibitor formation 32P-enzyme; lost when consumed. The phosphorylated has molecular weight 34,000, which...
Wheat germ supernatant is resolved into four fractions, C3cu, C3p, D, and A, all of which are required in addition to elongation factors EFl EF2 for tobacco mosaic virus-RNA (TMV-RNA)-catalyzed amino acid polymerization.Fractions C3/3 D function the binding Met-tRNA, Met 40 S ribosomal subunit forming an unstable S.Met-tRNA,"'"' complex that detected sucrose gradients only after fixation with glutaraldehyde.Fraction C38 binds Met-tRNAi 'let suggesting a C3p .Met-tRNAiMe"
A protein synthesis initiation factor of molecular weight 21,500 has been purified to homogeneity from extracts wheat germ. The factor, referred as D2d, is stable heating for 5 min at 75 degrees C and loses less than 15% its activity upon a similar treatment 90 C. In contrast, all other germ factors are inactivated by 65 Formation 80 S ribosome . Met-tRNAiMet complexes 40 complexes, measured the binding [3H]Met-tRNAiMet, almost completely dependent presence component D2d. shift in...
Abstract Incubating ribosomes and eukaryotic initiation factor eIF3 with an inosine-substituted mRNA (where the secondary structure is strongly reduced) in absence of ATP other protein synthesis factors produces a 40 S ribosome.mRNA complex. When Met-tRNAMeti eIF2 are added, 60 ribosome subunit attaches forming 80 three factors, eIF4B, cap-site factor, eIF4A, stimulate attachment subunit. In Met-tRNAMeti, 60-S does not attach, adding inhibits accumulation ribosome.inosine complexes. These...
Abstract PIEZO1 is critical to numerous physiological processes, transducing diverse mechanical stimuli into electrical and chemical signals. Recent studies underscore the importance of visualizing endogenous activity localization understand its functional roles. To enable physiologically clinically relevant on human PIEZO1, we genetically engineered induced pluripotent stem cells (hiPSCs) express a HaloTag fused PIEZO1. Combined with advanced imaging, our chemogenetic platform allows...