A5091071806- Trypanosoma species research and implications
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Cardiomyopathy and Myosin Studies
- Toxoplasma gondii Research Studies
- Photoreceptor and optogenetics research
- Biochemical and Molecular Research
University of Dundee
2016-2024
Wellcome Centre for Anti-Infectives Research
2021
University of Cambridge
2013-2016
University of South Australia
2012-2016
Pennsylvania State University
2016
The nuclear envelope (NE) separates translation and transcription is the location of multiple functions, including chromatin organization nucleocytoplasmic transport. molecular basis for many these functions have diverged between eukaryotic lineages. Trypanosoma brucei, a member early branching lineage Discoba, highlights these, distinct lamina kinetochore composition. Here, we describe cohort proteins interacting with both NPC, which term lamina-associated (LAPs). LAPs represent diverse...
Dinoflagellates are algae of tremendous importance to ecosystems and public health. The cell biology genome organization dinoflagellate species is highly unusual. For example, the plastid genomes peridinin-containing dinoflagellates encode only a minimal number genes arranged on small elements termed "minicircles". Previous studies peridinin have found evidence for divergent sequence evolution, including extensive substitutions, novel insertions deletions, use alternative translation...
The eukaryotic flagellum/cilium is a prominent organelle with conserved structure and diverse functions. Euglena gracilis, photosynthetic highly adaptable protist, employs its flagella for both locomotion environmental sensing. Using proteomics of isolated E. gracilis we identify nearly 1700 protein groups, which challenges previous estimates the complexity motile flagella. We not only identified several unexpected similarities shared mammalian flagella, including an entire glycolytic...
In trypanosomatids, transcription is polycistronic and all mRNAs are processed by trans-splicing, with export mediated noncanonical mechanisms. Although mRNA central to gene regulation expression, few orthologs of proteins involved in higher eukaryotes detectable trypanosome genomes, necessitating direct identification protein components. We previously described conserved pathway components Trypanosoma cruzi, including Sub2, a component the TREX complex, eIF4AIII (previously Hel45), core...
Eukaryotic cells arose ~1.5 billion years ago, with the endomembrane system a central feature, facilitating evolution of intracellular compartments. Endomembranes include nuclear envelope (NE) dividing cytoplasm and nucleoplasm. The NE possesses universal features: double lipid bilayer membrane, pore complexes (NPCs), continuity endoplasmic reticulum, indicating common evolutionary origin. However, levels specialization between lineages remains unclear, despite distinct mechanisms...
The dinoflagellates are an extremely diverse group of algae closely related to the Apicomplexa and ciliates. Much work has previously been undertaken determine presence various biochemical pathways within dinoflagellate mitochondria. However, these studies were unable identify several key transcripts including those encoding proteins involved in pyruvate dehydrogenase complex, iron-sulfur cluster biosynthesis, protein import. Here, we analyze draft nuclear genome Symbiodinium minutum, as...
Abstract Post-translational modifications (PTMs) modulate protein function, with ubiquitylation a pre-eminent example major roles in turnover. Ubiquitylation utilises ligase enzyme cascade for conjugation of ubiquitin to client proteins and cullin-RING ligases are amongst the most complex known. We reconstructed evolution E3 across eukaryotes experimentally characterised two cullin complexes trypanosomatids, taxon highly divergent from animals fungi. find considerable diversity within...